Sodium peroxide

Flaxseed oil cake (FOC) obtained via cold press technique was kindly donated by ACS Sp. z o.o. (Bydgoszcz, Poland) . The proximate composition of FOC was as follows: solids content 80.50%, protein content 41.97%, carbohydrates content 27.99%, fiber content 6.29%, fat content 6.11%, ash content 4.50%. Lactobacillus rhamnosus GG (ATCC53103), Enterococcus faecalis ATCC29212, Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC9027, Salmonella enterica subsp. enterica serovar Typhimurium ATCC14028, Staphylococcus aureus ATCC 43300 (MRSA -Methicillin-resistant Staphylococcus aureus) were purchased from ATCC (Manassas, Virginia, USA). Buffered peptone water, MRS agar and broth, XLD agar, Slanetz and Bartley agar, McConkey agar, Pseudomonas agar base with CN supplement, Mannitol Salt agar were obtained from Merck (Darmstadt, Germany). Phenolphthalein, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Folin-Ciocalteu's reagent, sodium carbonate, gallic acid, sodium nitrite, aluminium chloride, quercetin, and potassium persulphate were purchased from Sigma Aldrich (Darmstadt, Germany). Sodium chloride, sodium peroxide, ethanol, methanol, glucose, and fructose were supplied from Chempur (Piekary Śląskie, Poland). All reagents were of analytical grade.