Foxp3 pe

Manufactured by R&D Systems

FoxP3-PE is a flow cytometry reagent that detects the transcription factor Forkhead box P3 (FoxP3). FoxP3 is a key regulator of regulatory T cell (Treg) development and function. The FoxP3-PE reagent can be used to identify and quantify Treg cells in a variety of samples.

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Lab products found in correlation

Cd4 pe cy7, by Beckman Coulter (1 mentions) Cd25 pc5, by Beckman Coulter (1 mentions) Il 13, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Il 12, by Thermo Fisher Scientific (1 mentions) Mouse il 4, by Thermo Fisher Scientific (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions) Tgf β, by Thermo Fisher Scientific (1 mentions) Viability live dead ef650, by Thermo Fisher Scientific (1 mentions) Cd4 percp, by R&D Systems (1 mentions) Ly6g pe, by R&D Systems (1 mentions)

2 protocols using foxp3 pe

Profiling Regulatory T Cell Phenotypes

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For Treg and related cytokine analyses, stimulated PBMCs were stained to evaluate the expression of markers with CD4-PE-CY7 (Clone SK3) and CD25-PC5 (Beckman Coulter) antibodies (15 min at 4 °C in the dark). After incubation, the cells were fixed, permeabilized, and stained using the eBioscience™ Human Regulatory T Cell Staining Kit (Invitrogen, USA) according to the manufacturer’s instructions. Finally, the cells were stained with intracellular antibodies including BCL-2-FITC (Clone 124), FoxP3-PE (Clone PCH101), IL-10-APC (Clone JES3-9D7), IL-35-AF700 (R&D Systems), and transforming growth factor (TGF)-β-BV421 (Clone TW7-16B4), in accordance with the previous approach outlined in the FCM section.

Liu L., Cheng X., Yang H., Lian S., Jiang Y., Liang J., Chen X., Mo S., Shi Y., Zhao S., Li J., Jiang R., Yang D.H, & Wu Y. (2022). BCL-2 expression promotes immunosuppression in chronic lymphocytic leukemia by enhancing regulatory T cell differentiation and cytotoxic T cell exhaustion. Molecular Cancer, 21, 59.

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Multicolor Flow Cytometry for Immune Profiling

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The antibodies used were as follows: Ly6C-FITC (AL-21), CD68-PE (FA11), IL-17A-PE (TC11-18H10), CD45-PerCP (30-F11), CD11b-PB (M1/70.15), IFNγ-eFluor 450 (XMG1.2), CD4-PerCP (L3T4), F4/80-APC-eFluor780 (BM8), Foxp3-PE (MF23), CD3-APCCY7/eFluor780 (17A2), Ly-6G-PE (1A8), anti-mCCR2- FITC (R&D Systems), and anti-mouse CD16/CD32 (The Lymphocyte Culture Centre, UVA, Charlottesville, VA). To distinguish between live and dead cells, Viability Live Dead-ef650 (eBioscience, San Diego, CA) was used. Recombinant proteins used were as followed: mouse IL-4, IL-13, IL-12, IFNγ, TGFβ were purchased from PeproTech (Rocky Hill, NJ). LPS was purchased through Sigma-Aldrich (St. Louis, MO).

Taghavie-Moghadam P., Gjurich B., Jabeen R., Krishnamurthy P., Kaplan M.H., Dobrian A.D., Nadler J.L, & Galkina E.V. (2015). STAT4 Deficiency Reduces the Development of Atherosclerosis in mice. Atherosclerosis, 243(1), 169-178.

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