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Shark cartilage chondroitin sulphate

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Shark cartilage chondroitin sulphate is a natural compound extracted from shark cartilage. It is a key structural component of cartilage and is involved in maintaining the integrity and function of joint tissues.

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Lab products found in correlation

Papain, by Sangon (2 mentions)

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Chondroitin sulphate (26 citations) Shark chondroitin sulphate (3 citations) Chondroitin sulphate sodium salt from shark cartilage (2 citations)

2 protocols using shark cartilage chondroitin sulphate

1

Quantifying GAG Synthesis in NP Cells

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The DMMB assay was performed to measure GAG synthesized by NP cells [41 (link)]. Briefly, after DBM samples were suspended in 1 mL of PBS containing 5 mg/mL papain (Sangon, Biotech Co., Ltd., Shanghai, China) for 6–8 hours at 60 °C. Then, the GAG content in the digested sample was measured at an absorbance value at 525 nm via the 1,9-dimethyl methylene blue (DMMB) assay, in which shark cartilage chondroitin sulphate (Sigma-Aldrich) was used as a standard.
Li P., Hou G., Zhang R., Gan Y., Xu Y., Song L, & Zhou Q. (2017). High-magnitude compression accelerates the premature senescence of nucleus pulposus cells via the p38 MAPK-ROS pathway. Arthritis Research & Therapy, 19, 209.
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2

Quantification of GAG Content in NP Cells

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Glycosaminoglycan (GAG) content of the cultured NP cells was quantitated by 1,9-dimethyl methylene blue (DMMB) assay as described in a previous study [23 (link)]. Briefly, the cultured NP cells were collected and digested by 5 mg/ml papain (Sangon, Biotech Co., Ltd., China) for 6 h at 60°C. Then, the GAG content in the digested solution was calculated according to the absorbance value at 525 nm. Additionally, the shark cartilage chondroitin sulphate (Sigma, U.S.A.) was used as a standard in the DMMB assay.
Gao J., Zhang Q, & Song L. (2018). Resveratrol enhances matrix biosynthesis of nucleus pulposus cells through activating autophagy via the PI3K/Akt pathway under oxidative damage. Bioscience Reports, 38(4), BSR20180544.
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