Anti usp11 ab109232

Specific antibodies against p53 (DO-1, sc-126), p15 (D-12, sc-271,791), p19 (SPM429, sc-65,594) and RanBPM (sc-271,727) antibodies were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA), and p18 (#2896), p21 (#2947), p57 (#2557) and RanBPM (#14,638) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-p16 (A301-267 A) was purchased from Bethyl Laboratories (Montgomery, TX, USA). Anti-p27 (AF1669), anti-ATM (AF1399) and anti-phospho-ATM (Ser1981) were purchased from Beyotime Biotechnology (Shanghai, China). Anti-USP11 (ab109232) and anti-RanBPM (ab205954) antibodies were purchased from Abcam (Shanghai, China). Anti-GAPDH (AT0002) antibodies were purchased from CMCTAG (Milwaukee, WI, USA). Anti-Flag (cat. M185-3 L), anti-Myc (cat. M192-3) and anti-HA (cat. M180-3) antibodies were purchased from Medical & Biological Laboratories CO., LTD. (Minato-ku, Tokyo, Japan). Etoposide (VP-16, S1225) and KU-55,933 (S1092) were purchased from Selleck Chemicals (Shanghai, China), and Cycloheximide (sc-3508B) and MG132 (C2211) were purchased from Sigma (Shanghai, China). Doxorubicin (SC0159) was purchased from Beyotime Biotechnology (Shanghai, China).

The following primary antibodies were purchased from Cell Signaling Technology (Danvers, USA): anti-cleaved Notch1 (4147s; 1:1,000), anti-Hes1 (11988s; 1:1,000), p-eNOS (9570s, 1:1,000), eNOS (32027s, 1:1,000), iNOS (13120s,1:1,000), anti-AKT (4691s; 1:1,000), anti-phospho-AKT antibody (Ser473) (4060s; 1:1,000), anti-PTEN antibody (9559s; 1:1,000), anti-Actin antibody (4970s; 1:1,000), and anti-USP8 (11832s; 1:1,000). The following primary antibodies were obtained from Abcam (Waltham, USA): anti-USP11 (ab109232; 1:1,000), anti-VE-Cad antibody (ab33168; 1:1,000), and anti-Zo-1 antibody (ab216880; 1:1,000). LPS (L4516) and interleukin 6 (IL-6) were acquired from Sigma-Aldrich Merck KGaA (Germany). Melatonin and tangeretin (TGN) were purchased from Aladdin (purity >95%; Aladdin, China).