At 24 h following MCAO, PKD2-KI and WT mice were anesthetized with 1% isoflurane and transcardially perfused with 1× PBS followed by 4% paraformaldehyde in PBS. Brains were harvested and cryoprotected in 30% sucrose in PBS, and frozen serial coronal brain sections (35 μm thick) were prepared on a cryostat (CM1850 UV, LEICA). Brain sections were blocked with 5% normal goat serum in PBS for 1 h, followed by overnight incubation (4 °C) with the following primary antibodies: rabbit anti-NeuN (1:500; EMD Millipore), anti-PKD2 (1:200), anti-p-S876-PKD2 (1:200), and counterstained with DAPI. Secondary antibodies: Cy3-conjugated goat anti-rat IgG, Alexa Fluor 488-conjugated goat anti-rabbit IgG and goat anti-mouse IgG (all at 1:500; The Jackson ImmunoResearch Lab). Images were collected by confocal microscopy (FV1000-II; Olympus) and processed in Adobe Photoshop for compositions.
Fv1000 2
Manufactured by Olympus
Sourced in Japan
The FV1000-II is a high-performance confocal laser scanning microscope designed for advanced biological and materials research. It features a modular design, allowing for flexible configuration to meet the specific needs of the user. The FV1000-II provides high-resolution imaging capabilities and is capable of capturing detailed images of a wide range of samples.
Automatically generated - may contain errors
Lab products found in correlation
Cm1850 uv, by Leica (1 mentions)
Rabbit anti neun, by Merck Group (1 mentions)
Cy3 conjugated goat anti rat igg, by Jackson ImmunoResearch (1 mentions)
Rabbit anti vegfr2 antibody, by Cell Signaling Technology (1 mentions)
Rat anti cd31, by BD (1 mentions)
Alexa fluor 488 conjugated goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
2 protocols using fv1000 2
1
Immunohistochemical Analysis of PKD2 in Ischemic Stroke
2
Measuring Angiogenesis in Ischemic Muscle
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After completion of LSI, sham-operated and ischemic Malat1 WT and KO mice (n = 5 per group) were perfused with saline, and the gastrocnemius muscles were dissected out from both ischemic and contralateral hindlimb. Coronal sections (8 µm) were cryosectioned for immunostaining with rat anti-CD31 (BD Pharmingen, San Diego, CA, USA 1:200) or rabbit anti-VEGFR2 antibody (Cell Signaling Technology, Danvers, MA, USA, 1:200). Secondary antibodies: Cy3-conjugated goat anti-rat IgG, Alexa Fluor 488-conjugated goat anti-rabbit IgG (all at 1:1000; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA). Images were collected on confocal microscope (FV1000-II; Olympus; Tokyo, Japan) and processed in Adobe Photoshop (CS2 version 9.0, Adobe Systems, San Jose, CA, USA) for compositions [56 (link)].
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