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2 protocols using insulin from bovine

1

C2C12 Myoblast Culture Protocol

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Pyrrole and dimethyl sulfoxide (DMSO) were procured from Acros Organics (Thermo Fisher Scientific, Geel, Belgium). Dow Corning (Midland, MI, USA) supplied SYLGARDTM™184. Ammonium persulfate (APS), LiCl, and NaOH were obtained from Showa Kako (Osaka, Japan). Cytiva Hyclone (Cat. SH3003.02, Marlborough, MA, USA) provided Dulbecco's Modified Eagle Medium (DMEM), and Gibco (Thermo Fisher Scientific) supplied fetal bovine serum (FBS), horse serum, and trypsin. Additionally, rhodamine B, insulin from bovine, silicon oil AS 100, bovine serum albumin (BSA), formaldehyde, acetic acid, Triton‐X 100, 4′,6‐diamidine‐2′‐phenylindole dihydrochloride (DAPI), MTT, and phalloidin‐FITC were obtained from Sigma Aldrich (Merck, Darmstadt, Germany). C2C12 mouse myoblasts were obtained from the Bioresource Collection and Research Center (Hsinchu, Taiwan).
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2

Mammary Epithelial Cell Culture

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Dulbecco's modified Eagle medium (DMEM) was purchased from Invitrogen (Carlsbad, CA). Fetal bovine serum (FBS) was purchased from Equitech-Bio (Cotton Gin Lane, TX). Penicillin and streptomycin, hydrocortisone, insulin from bovine, and gentamicin and prolactin from sheep pituitary were purchased from Sigma-Aldrich (St. Louis, MO). Recombinant bovine growth hormone was purchased from Ray Biotech (Norcross, GA). All other compounds were from Nacalai Tesque (Kyoto, Japan).
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