β-MCA, ω-MCA, UDCA, HDCA, T-α-MCA, T-β-MCA, T-UDCA, and THDCA were purchased from Steraloids Inc. (Newport, RI). α-MCA, CA, DCA, CDCA, TCDCA, TDCA, and TCA were purchased from Sigma-Aldrich (St Louis, MO). Irinotecan was obtained from Sigma-Aldrich (St Louis, MO). All other reagents and solvents were of HPLC grade. Anti-mouse CD3 (clone 145-2C11), anti-mouse CD28 (clone 37.51), anti-mouse CD16/CD32 (clone 93), anti-mouse CD45 Alexa Fluor® 700 (Clone: 30-F11), anti-mouse CD4 eFluor® 450 (Clone: GK1.5), anti-mouse CD8α PE-Cyanine7 (Clone: 53-6.7), anti-mouse CD8β FITC (Clone: eBioH35-17.2), anti-mouse TCRβ PerCP-Cyanine5.5 (Clone: H57-597), anti-mouse/rat Foxp3 APC (Clone: FJK-16s), anti-mouse IL-10 APC (Clone: JES5-16E3) were from eBiosciences (San Diego, CA). Cytofix/Cytoperm Fixation/ Permeabilization Solution and Golgi Plug protein transport inhibitor were purchased from BD Biosciences (Franklin Lakes, NJ). Foxp3 Fix/Perm Buffer Set and mouse IL-10 ELISA kit were purchased from BioLegend (San Diego, CA).
Anti mouse cd45 alexa fluor 700
Manufactured by Thermo Fisher Scientific
The Anti-mouse CD45 Alexa Fluor® 700 is a fluorochrome-conjugated antibody that specifically binds to the CD45 antigen expressed on mouse leukocytes. It is designed for use in flow cytometry applications to identify and analyze various mouse immune cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Cytofix cytoperm fixation permeabilization solution, by BD (1 mentions)
Irinotecan, by Merck Group (1 mentions)
Tcdca, by Merck Group (1 mentions)
Tαmca, by Steraloids (1 mentions)
Anti mouse cd4 efluor450, by Thermo Fisher Scientific (1 mentions)
T β mca, by Steraloids (1 mentions)
T udca, by Steraloids (1 mentions)
T hdca, by Steraloids (1 mentions)
Anti mouse il 10 apc, by Thermo Fisher Scientific (1 mentions)
Mouse il 10 elisa kit, by BioLegend (1 mentions)
Golgiplug protein transport inhibitor, by BD (1 mentions)
Anti mouse rat foxp3 apc, by Thermo Fisher Scientific (1 mentions)
Foxp3 fix perm buffer set, by BioLegend (1 mentions)
Zombie nir fixable viability kit, by BioLegend (1 mentions)
Donkey anti rabbit alexafluor 647, by Abcam (1 mentions)
Lsrfortessa, by BD (1 mentions)
Anti mouse ly6g pecy7, by BioLegend (1 mentions)
2 protocols using anti mouse cd45 alexa fluor 700
1
Bile Acid Panel Profiling Protocol
2
Multicolor Flow Cytometry of Lung Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lung tissue homogenates were stained with the following conjugated antibodies: anti-mouse CD45 AlexaFluor700 (1∶300; eBioscience), anti-mouse Siglec-F AlexaFluor647 (1∶300; BD Bioscience), anti-mouse Ly6g PeCy7 (1∶400; BioLegend), anti-mouse/human CD11b BV605 (1∶2500; BioLegend), anti-mouse CD11c BV510 (1∶2000; BioLegend). A Zombie NIR fixable viability kit (Biolegend) was used to differentiate live cells from dead cells. To stain for CLR and RAMP1, rabbit anti-CLR (H42; Santa Cruz) and rabbit anti-RAMP1 (FL-148; Santa Cruz) primary antibodies were used, followed by a donkey anti-rabbit AlexaFluor647 (1∶200; Abcam) secondary. The samples were analyzed by flow cytometry on a BD LSRFortessa.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!