Apoptosis assays were conducted using the Annexin V-fluorescein isothiocyanate apoptosis kit (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s protocols. The results were analyzed with a Dxp Athena™ flow cytometer (Cytek, Fremont, CA, USA).
Annexin 5 fluorescein isothiocyanate apoptosis kit
Manufactured by BD
Sourced in United States
The Annexin V-fluorescein isothiocyanate apoptosis kit is a laboratory equipment product designed to detect and quantify apoptosis, a type of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, a marker of apoptosis, and fluorescein isothiocyanate (FITC), a fluorescent dye, to enable the visualization and analysis of apoptotic cells.
Automatically generated - may contain errors
Lab products found in correlation
Cell cycle analysis kit, by BD (3 mentions)
Facscalibur flow cytometer, by BD (2 mentions)
Facscanto 2 flow cytometer, by BD (2 mentions)
Dxp athena flow cytometer, by Cytek (1 mentions)
Facscan, by BD (1 mentions)
Fc500, by Beckman Coulter (1 mentions)
Facscalibur, by BD (1 mentions)
Facsaria 3 device, by BD (1 mentions)
Gallios flow cytometer, by Beckman Coulter (1 mentions)
10 protocols using annexin 5 fluorescein isothiocyanate apoptosis kit
1
Annexin V-FITC Apoptosis Assay
2
Detecting Cellular Apoptosis via Flow Cytometry
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For this experiment, 2×106 cells were plated into 60-mm dishes and then treated with or without CAE/DXM for 24 h. Cellular apoptosis was detected using the AnnexinV-fluorescein isothiocyanate Apoptosis kit (BD Biosciences, Franklin Lakes, NJ, USA) and a FACSCanto II flow cytometer (BD Biosciences), following the manufacturers protocol.
3
Apoptosis Assessment via AO/EB and Annexin V
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Acridine orange/ethidium bromide (AO/EB) double fluorescence staining was carried out to measure cell apoptosis. Briefly, the cells were treated with AO/EB mixed solution (Solarbio, Beijing, China). Five minutes later, the cells were counted and photographed (Leica, Wetzlar, Germany).
Annexin V‑fluorescein isothiocyanate apoptosis kit (BD Biosciences, San Jose, CA, USA) was applied to evaluate the apoptotic rate of HCC cells. In brief, the transfected cells (2 × 106) were washed with PBS twice, resuspended with 1X binding buffer, and then maintained with 5 μL of Annexin V-FITC and 10 μL of propidium iodide (PI) for 15 minutes in the dark. At last, cell apoptosis was detected using a flow cytometer (FACScan, BD Biosciences).
Annexin V‑fluorescein isothiocyanate apoptosis kit (BD Biosciences, San Jose, CA, USA) was applied to evaluate the apoptotic rate of HCC cells. In brief, the transfected cells (2 × 106) were washed with PBS twice, resuspended with 1X binding buffer, and then maintained with 5 μL of Annexin V-FITC and 10 μL of propidium iodide (PI) for 15 minutes in the dark. At last, cell apoptosis was detected using a flow cytometer (FACScan, BD Biosciences).
4
Annexin-V Apoptosis Assay by Flow
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Cell apoptosis was measured using the Annexin-V-Fluorescein Isothiocyanate Apoptosis kit (BD Biosciences, San Jose, CA, United States) based on flow cytometry analysis using the FACSCalibur flow cytometer, followed by quantification with FlowJo software.
5
Cell Cycle and Apoptosis Analysis
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The cell cycle analysis kit and annexin V-fluorescein isothiocyanate apoptosis kit were purchased from BD Biosciences (San Jose, CA, USA). For cell cycle analyses, cells were treated with theaflavins (40 µg/mL) for 48 hours, and then 1×106 cells were fixed in 70% ethanol at −20°C overnight. On the next day, the fixed cells were stained according to the protocol of the manufacturer and analyzed by flow cytometry (Beckman Coulter FC500; Beckman Coulter, Brea, CA, USA). For apoptosis analyses, cells were harvested at a concentration of 1×106 cells/mL after treatment with theaflavins (40 µg/mL) for 48 hours. The cells were then stained according to the protocol of the manufacturer and analyzed by flow cytometry using the Beckman Coulter FC500.
6
Cell Cycle and Apoptosis Analysis
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Cell cycle and apop-tosis assays were performed as described previously (34 (link)). Annexin V-fluorescein isothiocyanate apoptosis kit and cell cycle analysis kit (both from BD Biosciences, Franklin Lakes, NJ, USA) were used according to the manufacturer’s instructions. The cells were analyzed on a FACSCalibur flow cytometer (BD Biosciences).
7
Apoptosis and Cell Cycle Assay
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Apoptosis and cell cycle assays were measured by the Annexin V-fluorescein isothiocyanate apoptosis kit and cell cycle analysis kit (BD Biosciences, SanJose, CA, USA) according to the protocols. The results were analyzed with a FACSCalibur flow cytometer (BD Biosciences).
8
Detecting Synergistic Apoptosis Effects
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To detect synergistic effects of the chemotherapeutic drugs on cell apoptosis, treated cells were analyzed using the fluorescein isothiocyanate-Annexin V apoptosis kit (BD Biosciences, San Jose, CA, USA). The results were analyzed by flow cytometry using an FACSAria III device (BD Biosciences). All the experiments were repeated independently in triplicate.
9
Quantifying Apoptosis in Gastric Cancer Cells
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Cell apoptosis was assessed as previous protocols (42 (link)). Gastric cancer cells were treated with ARV-825 of different concentrations. At 72 h after treatment, the cells were trypsinized, centrifugated at 180g for 4 min and washed with PBS. The cells were suspended in binding buffer and stained using the fluorescein isothiocyanate-Annexin V apoptosis kit (Cat. 556420, BD Biosciences). The cell apoptosis was tested and analyzed by a Beckman Gallios™ flow cytometer.
10
ARID1A Knockdown and Apoptosis Induction
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Cells were seeded in 6-well plates at a density of 5 × 10 5 cells/well 1 day after siRNA-mediated knockdown of ARID1A and incubated at 37 °C. GSK2110183 (10 µM) was added when most cells had attached to the well. After further incubation for 48 h, treated cells were analyzed using the fluorescein isothiocyanate-Annexin V apoptosis kit (BD Biosciences, San Jose, CA, USA). The results were analyzed with a BD FACSCanto II flow cytometer (BD Biosciences). Flow cytometry data were analyzed using FlowJo 10.7.1 (FlowJo, Ashland, OR, USA, proprietary commercial software, https:// www. fowjo. com/) [24] .
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