Approximately 4 × 104 cells (in 100 μL serum free medium) were seeded on the upper chambers of a 24-well format cell culture insert with 8 μm pores (Corning, Tewksbury, MA, USA). The lower chambers were filled with 600 μL complete medium containing 20 ng/mL EGF (Sino Biological). After incubation at 37°C for 36 h, the migrating cells were fixed with 4% paraformaldehyde, stained with 0.5% crystal violet, and then counted in five randomly chosen microscope fields per filter under a light microscope (Olympus, Tokyo, Japan) at ×100 magnification.
24 well format cell culture insert
Manufactured by Corning
Sourced in United States
The 24-well format cell culture insert is a laboratory equipment designed for in vitro cell culture experiments. It provides a multi-well platform for the study of cellular processes and interactions. The insert establishes a separation between the upper and lower chambers, enabling the examination of factors that influence cell growth and behavior.
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Lab products found in correlation
2 protocols using 24 well format cell culture insert
1
Cell migration assay using EGF
2
Cell Migration Assay Protocol
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Trypsin-digested cells were resuspended in serum-free medium at a density of 1 × 106/mL, and 400 μL of cell suspension was added to a 24-well format cell culture insert (Corning, NY, USA). Subsequently, 1 mL of medium with 20% serum was added to the lower chamber, the cells were rinsed with PBS after 4 days, fixed for 15 min with 4% PFA, and stained with crystal violet solution (Servicebio, Wuhan, China) for 10 min. Thereafter, cotton swabs were used to gently wipe off the cells in the upper chamber, and we placed the samples under a microscope for observation (Zeiss, Germany).
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