Flir t650sc

Manufactured by Teledyne

Sourced in United States

The FLIR T650sc is a high-performance infrared camera designed for scientific and industrial applications. It features a 640 x 480 resolution infrared detector and can capture thermal images with high accuracy and sensitivity. The camera is capable of measuring temperatures ranging from -40°C to +2,000°C and offers advanced features such as radiometric imaging, on-camera analysis tools, and wireless connectivity.

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Lab products found in correlation

Bat 12 microprobe thermometer, by Physitemp (1 mentions) Thermacam researcher pro 2, by Teledyne (1 mentions)

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T650sc (17 citations) FLIR T650Sc thermal camera (2 citations)

9 protocols using flir t650sc

Infrared Tail Skin Temperature Measurement

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Rats were restrained in a holder in a conscious state, and their TST at the dorsal surface of the tail about 2 cm from the fur line was measured for 15 min using an infrared camera (Flir T650sc, FLIR Systems Inc., Portland, USA). Before testing, all animals were settled in the laboratory room for 15 min at an environmental temperature of 25°C. In the following image analysis, the highest temperature was recorded after designating the region of interest at the dorsal surface of the tail about 2 cm from the fur line of the rat using the ResearchIR v4 program. Respectively, TST data were measured at 1-min intervals throughout the experimental period. The mean TST during the 15 min of the measurement period was calculated, and the data were analyzed as the change in the mean TST for each 15 min measurement compared with the mean TST at 0 min. Changes in TST were assessed using ΔTST.
Values were expressed as means ± standard error (S.E.)

Lee G., Shin J., Jo A., Lm S., Kim M.R., Shoi Y., Yun H., Bae D., Kim J, & Choi C.Y. (2020). Antipostmenopausal effects of Stauntonia hexaphylla and Vaccinium bracteatum fruit combination in estrogen-deficient rats. Food & Nutrition Research, 64, 10.29219/fnr.v64.5233.

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Substrate-Supported Tattoo-Like Electrodes

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The substrate-supported large-area tattoo-like electrodes covered the participant’s full right forearm and the substrate-free electrodes were attached on the counterpart location of the left forearm at 10 min. During states I and II, the participant rested in a room under room temperature 25°C. After normal outdoor running (about 10 min at 37°C), the participant rested in the same room at state III. At about 160 min, both of devices were removed. The temperature during the whole process was captured by an IR camera (FLIR T650SC, USA). The PU film is opaque to IR light so that the raw data of electrodes with substrates were corrected to obtain the skin temperature, seen in the Supplementary Materials.

Wang Y., Yin L., Bai Y., Liu S., Wang L., Zhou Y., Hou C., Yang Z., Wu H., Ma J., Shen Y., Deng P., Zhang S., Duan T., Li Z., Ren J., Xiao L., Yin Z., Lu N, & Huang Y. (2020). Electrically compensated, tattoo-like electrodes for epidermal electrophysiology at scale. Science Advances, 6(43), eabd0996.

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Facial Flushing Verification in Menopausal Rats

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To verify the efficacy of the extracts on facial flushing, the surface temperatures of the tail of the ovariectomy-induced menopausal rats showing a vascular response similar to that of human skin tissue was measured. The experiment was designed based on a previous study [32 (link)]. The facial flushing verification test group was raised in a polycarbonate cage ([W] 26 × [D] 42 × [H] 18 cm). When the housed rats were moved to a cage for measurement ([W] 40 × [D] 80 × [H] 40 cm) and accommodated in groups, stress occurred between individuals owing to environmental changes and pecking-order disputes. This stress raised the tail surface temperature in the OVX rat models, inducing an effect similar to facial flushing in humans. In this experiment, after 30 min of oral extract administration, the rats were moved to a cage for measurement and then photographed using an infrared camera (Flir T650sc; FLIR Systems, Stockholm, Sweden) for 1 h. The images were analyzed using the ResearchIR v4 program by recording the highest temperature after designating a region of interest at a point 3 cm away from the end of the tail hair of the rats. The analysis was performed based on the results measured up to 15 min, as 0 to 3 min was the stabilization time, showing irregular temperature changes and reduced movement of the rats. The temperature decreased after 15 min.

Park D., Yoon J.E., Choi B., Lee Y.J, & Ha I.H. (2023). Complex Extract of Polygonatum sibiricum and Nelumbinis semen Improves Menopause Symptoms via Regulation of Estrogen Receptor Beta in an Ovariectomized Rat Model. Nutrients, 15(11), 2443.

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Quantifying Interscapular Brown Fat Activity

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The temperature over interscapular BAT (iBAT) was measured for 10 consecutive days using a thermal camera (FLIR T-650sc, FLIR Systems, Wilsonville, OR, USA) after 4 h of fasting to avoid BAT activation after a meal. The researchers were “blinded” to the group of animals that was scanned since the estrous cycle was determined after the measurements were performed. The interscapular area of the animals was shaved the day before the experiment and additionally when necessary during the 10 days of the experiments. For measurements, each animal was placed in a clean polystyrene cage. Animals were allowed to adjust to new environment and filmed with thermal camera for 30 s. The camera software calculates the object’s temperature by taking into account the emissivity of mouse skin (e = 0.97), reflected room temperature (24.8 ± 0.8 °C), air temperature (24.2 ± 0.6 °C), relative humidity (60.2 ± 4.4%, n = 16), and distance to the object (1 m) as these values can affect the measurements. These values did not differ between the tested groups.
To determine BAT activity we calculated iBAT heat energy output in watts (W) with the Stefan-Boltzmann law (17 (link)):
where ϵ is emissivity of 0.97, σ is Stefan-Boltzmann’s constant (5.676 × 10⁻⁸ Wm² K⁴), A is an area of the skin warmer than 34.6 °C (region of interest) in m², and T is the mean temperature of the area in K.

Ratko M., Habek N., Kordić M, & Dugandžić A. (2020). The use of infrared technology as a novel approach for studies with female laboratory animals. Croatian Medical Journal, 61(4), 346-363.

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Thermographic Monitoring of Endodontic Procedures

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A FLIR T650sc (Flir, Wilsonville, OR, USA) handheld camera with an infrared sensor, 25-mm lens and spatial resolution of 640 × 80 pixels was used to capture the thermographic images (thermograms). During thermogram acquisition, room temperature was maintained at 23 ± 1°C, and the relative humidity of the air between 40% and 60%.
To avoid thermal interference, an apparatus to hold and isolate the sample was built, consisting of a thermal box of expanded polystyrene plates, the internal walls of which were coated with a layer of aluminum foil covered with black ethylene vinyl acetate (EVA). To limit the field of view uniquely to the sample, a rectangular opening was made at the point where the images were to be captured (Fig. 1).
The IT camera was positioned 30 cm from the sample. The camera was programmed to continually acquire images at 15 s intervals immediately before the start of the endodontic treatment until the temperature had normalized. The thermal camera was set at 0.98 emissivity and 44% relative humidity.
The measured temperature values were considered to represent the increase from the initial temperature (temperature at the established worktime minus the initial temperature of the sample before the start of endodontic filling). The time taken for the root temperature to normalize was also measured.

Suassuna F.C.M., de Araújo D.K.M., Amorim A.M.A.M., Melo S.L.S., Heck R.J., Antonino A.C.D., Bento P.M., Silva D.F.B, & de Melo D.P. (2023). Thermal and volumetric assessment of endodontic filling techniques using infrared thermography and micro-CT. Journal of oral science, 65(1).

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Combination Therapy for Ovarian Cancer

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For tumor progression studies, nude mice were implanted with ~1-2 × 10⁶ CP70 human ovarian cancer cells in the hind flanks. Tumor treatments consisted of administration of PEG-NRs, doxorubicin-loaded liposomes (5 mg/kg) (Encapsula), and NIR irradiation with appropriate controls. Briefly, 48-72 hours after PEG-NR administration, tumors were exposed to NIR laser irradiation to achieve a temperature of ~41-43°C for 30 minutes as monitored using an infrared camera (FLIR T650sc). 24 hours later, animals underwent a second cycle of NIR laser irradiation in combination with intravenously administered doxorubicin-loaded liposomes. Longitudinal tumor progression studies lasted ~4-5 weeks from initial treatment with tumor volumes measured approximately every 3-4 days using digital calipers by investigators who were blinded to the experimental groups. Animals were euthanized when the tumor burden exceeded 500 mm³. Plots and statistical analysis of tumor volume growth and Kaplan-Meier survival analysis were performed using GraphPad Prism.

Bagley A.F., Scherz-Shouval R., Galie P.A., Zhang A.Q., Wyckoff J., Whitesell L., Chen C.S., Lindquist S, & Bhatia S.N. (2015). Endothelial thermotolerance impairs nanoparticle transport in tumors. Cancer research, 75(16), 3255-3267.

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Wheat Canopy Thermal Imaging Workflow

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An FLIR T650sc thermal infrared camera (FLIR Systems, Inc., Wilsonville, OR, USA) was used to obtain the wheat canopy temperature (CT) and RGB images. The device has dual thermal infrared and visible light sensors, and the image resolution is 640 × 480 pixels. Synchronous with the spectral reflectance measurement, the lens was 1.0 m from the top of the wheat crop, and the thermal infrared and RGB images were obtained vertically (Figure 1).

Feng Z., Song L., Duan J., He L., Zhang Y., Wei Y, & Feng W. (2021). Monitoring Wheat Powdery Mildew Based on Hyperspectral, Thermal Infrared, and RGB Image Data Fusion. Sensors (Basel, Switzerland), 22(1), 31.

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Measuring Mouse Skin Temperature

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Skin temperature of mice was determined with thermal camera (FLIR T650SC, FLIR Systems). Rectal temperatures were measured with thermometers (BAT-12 Microprobe Thermometer, Physitemp Instruments) before and during 4°C cold exposure.

Kang G.M., Min S.H., Lee C.H., Kim J.Y., Lim H.S., Choi M.J., Jung S.B., Park J.W., Kim S., Park C.B., Dugu H., Choi J.H., Jang W.H., Park S.E., Cho Y.M., Kim J.G., Kim K.G., Choi C.S., Kim Y.B., Lee C., Shong M, & Kim M.S. (2021). Mitohormesis in hypothalamic POMC neurons mediates regular exercise-induced high-turnover metabolism. Cell metabolism, 33(2), 334-349.e6.

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Infrared Thermography of Kid Goat Horn Buds

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Images of the horn buds were taken using a handheld infrared thermography camera (FLIR T650sc; FLIR Systems Inc., Wilsonville, OR). Two images were taken immediately before treatment (0900 h) and again at 24, 36, and 72 h post-treatment (~1500 h), and the clearest image of the pair at each sampling point was selected for analysis. Images were not taken within 24 h of treatment to avoid handling and associated stress affecting other measures. Kids were restrained so that the head remained horizontal to the floor, and images of the horn bud area were taken (~3-cm diameter around each horn bud). Handlers took care not to touch the horn bud area during restraint. All images were taken at 1 m directly in front of the animal. Ambient temperature (°C) and relative humidity (%) within the facility were recorded continuously using a data logger (EL-USB-2-LCD+, Lascar Electronics, Salisbury, UK), and temperature and humidity data were entered into the ThermaCam Researcher Pro 2.10 software (FLIR Systems AB, Täby, Sweden) used for thermal image analysis. The maximum skin surface temperature (°C) was calculated from an approximately 2-cm-diameter area surrounding each horn bud.

Hempstead M.N., Waas J.R., Stewart M., Cave V.M, & Sutherland M.A. (2018). Evaluation of alternatives to cautery disbudding of dairy goat kids using physiological measures of immediate and longer-term pain. Journal of dairy science, 101(6).

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