M8028

Manufactured by Merck Group

Sourced in United States

The M8028 is a laboratory equipment product from Merck Group. It is designed for precise measurement and analysis tasks in research and industrial settings. The core function of the M8028 is to provide accurate and reliable data collection and processing capabilities for various scientific applications. For a more detailed and unbiased description, please consult the product's technical documentation.

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Lab products found in correlation

Mcdb 201 medium, by Merck Group (1 mentions) Phg0311l, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Collagenase type 2, by Worthington (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)

5 protocols using m8028

Heparan Sulfate Removal Enhances SARS-CoV-2 Infection

Cited 1 time

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Removal of cell surface heparan sulfate by sodium chlorate treatment was performed as before (Robinson-McCarthy et al., 2018 (link)). In brief, H522 cells were passaged in sulfate-free Joklik modified minimal essential medium (M8028, Sigma-Aldrich, St. Louis, MO) with 20% FBS with or without 50mM sodium chlorate for two passages prior to seeding for infection. SARS-CoV-2 infections were performed in the presence of sodium chlorate. We confirmed the ability of H522 cells supporting SARS-CoV-2 infection following switching cells back to regular RPMI media, arguing against clonal selection of a subpopulation of cells following sodium chlorate treatment. As an alternative, H522 cells were treated with a combination of heparinase I (5.8 μg/mL), II (11 μg/mL) and III (11.2 μg/mL). In brief, herparinases were reconstituted per manufacturer’s recommendation and cells seeded in 24-well plates were treated with the enzymes in 200 μL media containing heparinases for 90 minutes at 37°C. Virus inoculum (MOI: 0.1 IU/cell) was added on top and incubated for 1h at 37°C for adsorption. Virus inoculum and heparinases were removed, wells washed with PBS and replaced with RPMI-10%FBS.

Puray-Chavez M., LaPak K.M., Schrank T.P., Elliott J.L., Bhatt D.P., Agajanian M.J., Jasuja R., Lawson D.Q., Davis K., Rothlauf P.W., Liu Z., Jo H., Lee N., Tenneti K., Eschbach J.E., Shema Mugisha C., Cousins E.M., Cloer E.W., Vuong H.R., VanBlargan L.A., Bailey A.L., Gilchuk P., Crowe JE J.r., Diamond M.S., Hayes D.N., Whelan S.P., Horani A., Brody S.L., Goldfarb D., Major M.B, & Kutluay S.B. (2021). Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell. Cell Reports, 36(2), 109364.

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Isolation of Primary Pulmonary Cells from Newborn Mice

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Newborn ICR mice were obtained from National Laboratory Animal Center and National Applied Research Laboratories (Taipei, Taiwan). Primary pulmonary cells were isolated as described previously (Ling et al., 2006 (link)). Briefly, lung tissue was collected from 0 to 2 days postpartum newborn ICR mice. After washing with Hank’s buffer, tissue was cut into 5-mm pieces and digested with 10 mg/mL protease in Joklik’s MEM medium (M8028, Sigma-Aldrich, United States) at 4°C for 16 h. Tissues were transferred into 10% FBS Joklik’s MEM medium and filtered through a 100-μm nylon cell strainer. These cells were washed and resuspended in MCDB-201 medium (M6770, Sigma-Aldrich, United States). The cells were plated in collagen I (10 μg/cm²; 354236, BD Bioscience, United States) coated plates in MCDB-201 medium containing 1% Insulin-Transferrin-Selenium-A supplement (ITS-A, 51300-044, Gibco, United States), 1 ng/ml human EGF (PHG0311L, Gibco, United States), and 1% antibiotic solution (15240-062, Gibco, United States). After removal of the non-adherent cells after 48 h, cultures were maintained before further experiments. The detailed protocol had been described in previous studies (Ling et al., 2006 (link), 2014 (link)).

Chao T.L., Gu S.Y., Lin P.H., Chou Y.T., Ling T.Y, & Chang S.Y. (2020). Characterization of Influenza A Virus Infection in Mouse Pulmonary Stem/Progenitor Cells. Frontiers in Microbiology, 10, 2942.

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Isolation of Murine Cardiomyocytes

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Cardiomyocytes were isolated from wild type or mutant mice as previously described[5 (link)]. In brief, cardiomyocytes were isolated from 6- to 8-week-old mice. The hearts were removed and perfused retrogradely on a Langendorff apparatus for approximately 10 minutes with perfusion solution containing Minimal Essential Medium (MEM), Joklik modified (Sigma, M8028) with the following additions (all Sigma, in mmol/L): KHCO₃ 10, HEPES 10, taurine 30, L-carnitine 2, creatine 2, D-glucose 20 and collagenase type 2 (150 units/ml, Worthington). The heart was removed from perfusion when soft and white. Left and right ventricles were minced into small pieces and then allowed to digest for another 10 minutes in enzyme solution with frequent trituration at 37 °C. The solution was filtered through sterile 210 µm nylon and centrifuged at 1000 rpm for 3 minutes to pellet cells. The cells were resuspended in perfusion solution with bovine serum albumin (BSA, Sigma) at 5 mg/ml. Calcium tolerance was performed by gradually adding CaCl₂ to a final concentration of 1 mM. Rod-shaped, striated cells were used for electrophysiological recording and image collection.

Wang X., Tang H., Wei E.Q., Wang Z., Yang J., Yang R., Wang S., Zhang Y., Pitt G.S., Zhang H, & Wang C. (2017). Conditional knockout of Fgf13 in murine hearts increases arrhythmia susceptibility and reveals novel ion channel modulatory roles. Journal of molecular and cellular cardiology, 104, 63-74.

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Sodium Chlorate Enhances HERVK Infection

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BSRT7 cells were passaged in sulfate-free Joklik modified minimal essential medium (M8028, Sigma-Aldrich, St. Louis, MO) with 10% FBS with or without 50mM sodium chlorate for at least two passages prior to seeding for infection. Cells were infected with either VSV-HERVK or VSV at an MOI of 1 particle forming unit (PFU)/cell. Cells were collected 5hpi and fixed in 2% PFA and fluorescence measured using a FACSCalibur instrument. The % eGFP-positive cells and MFI were quantified using FlowJo software. Data are represented as fold difference in % eGFP-positive cells or MFI normalized to VSV infected cells. Error bars represent standard error of the mean from three independent biological replicates.

Robinson-McCarthy L.R., McCarthy K.R., Raaben M., Piccinotti S., Nieuwenhuis J., Stubbs S.H., Bakkers M.J, & Whelan S.P. (2018). Reconstruction of the cell entry pathway of an extinct virus. PLoS Pathogens, 14(8), e1007123.

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SILAC Labeling and Nuclear Extraction of HeLa S3 Cells

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HeLa S3 cells (ATCC CCL-2.2) were a gift of Melissa Jurica. They were grown in SMEM medium (M0518, Life Technology). The medium was supplemented with 2 mg/mL sodium bicarbonate, 4 mM glutamate, 1X nonessential amino acids (Life Technologies), 5% PBS-dialyzed newborn calf serum (Omega Scientific, Tarzana, California), 1X penicillin–streptomycin (Life Technologies). For SILAC experiments, customized medium (similar to M8028 of Sigma-Aldrich without sodium bicarbonate, glutamate, arginine and lysine) was prepared by AthenaES, Halethorpe, Maryland. 13C-arginine (R6) and 13C-lysine (K6) were purchased from Cambridge Isotope Laboratories, Tewksbury, Massachusetts. The complete medium was reconstituted as above and with either ‘light’ (R0K0) or ‘heavy’ (R6K6) arginine and lysine at 12.6 g/L and 7.25 g/L, respectively. HeLa S3 cells were grown in either R0K0 or R6K6 for five to six doubling cycles to obtain high labeling efficiency (>99%). The cells were harvested in log phase and processed for nuclear extraction as described previously (Black, 1992 (link); Dignam, 1990 (link)).

Wongpalee S.P., Vashisht A., Sharma S., Chui D., Wohlschlegel J.A, & Black D.L. (2016). Large-scale remodeling of a repressed exon ribonucleoprotein to an exon definition complex active for splicing. eLife, 5, e19743.

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