Ofloxacin powder

Manufactured by Merck Group

Sourced in United States

Ofloxacin powder is a synthetic antibacterial agent. It is a crystalline powder that can be used in pharmaceutical and laboratory applications.

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Ofloxacin (117 citations)

3 protocols using ofloxacin powder

Quantifying Rebamipide and Ofloxacin in Rat Urine

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Rebamipide powder (Tokyo Chemical Industry CO., Tokyo, Japan) (Cat. R0085). Ofloxacin powder (Sigma–Aldrich, St. Louis, MO, USA) (Cat. PHR1168-1G) HPLC grade methanol (Fisher Scientific, Pittsburg, PA, USA) (Cat. A9984). HPLC grade acetonitrile (Fisher Scientific, Pittsburg, PA, USA) (Cat. BP2405SK-4) 36% hydrochloric acid (Sigma–Aldrich, St. Louis, MO, USA) (Cat. 258148) HPLC grade water (Fisher Scientific, Pittsburg, PA, USA) (Cat. W5-4). HPLC grade glacial acetic acid (Fisher Scientific, Pittsburg, PA, USA) (Cat. A35-500). Drug free rat urine samples. Experimental urine samples from rats dosed with rebamipide.

Cooper D.L, & Harirforoosh S. (2014). A simple high performance liquid chromatography method for determination of rebamipide in rat urine. MethodsX, 1, 49-55.

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Synthesis and Characterization of Silver Nanoparticles

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Silver nitrate (99.9%), sodium citrate dehydrate (99%), sodium borohydride (98%), hydrogen peroxide (30%), polyvinylpyrrolidone (PVP), 2-mercaptoethanol (99%), 11-amino-1-undecanethiol hydrochloride (MUNH₂, 99%), ofloxacin powder (99%), orthovanadate (≥90%), sodium chloride, sodium phosphate, sodium phosphate monobasic monohydrate, Bacto-Tryptone, and Bacto Yeast Extract were purchased from Sigma-Aldrich. N-Hydroxysulfosuccinimide (Sulfo-NHS, 98.5%, Pierce), 1-ethyl-3-[3-dimethylaminopropyl]-carbodiimide hydrochloride (EDC, 99%, Pierce), silver perchlorate monohydrate (99%, Alfa Aesar), live/dead backlight viability assay (Life Technologies), and Hoechst 33342 (Life Technologies) were purchased as indicated, and used as received. The cell line of Escherichia coli (wt w3110) was purchased from a genetic stock center (CGSC). Deionized (DI) water (18 MΩ water, Barnstead) was used to prepare all the solutions including a standard LB medium (1% tryptone peptone, 0.5% yeast extract, and 0.5% NaCl, pH = 7.2) and a modified LB medium (1% tryptone peptone, 0.5% yeast extract, and 0.1% NaCl, pH = 7.2).

Songkiatisak P., Ding F., Cherukuri P.K, & Xu X.H. (2020). Size-dependent inhibitory effects of antibiotic nanocarriers on filamentation of E. coli. Nanoscale Advances, 2(5), 2135-2145.

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Bacterial Nanoparticle Synthesis Protocol

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Silver nitrate (99.9%), sodium citrate dehydrate (99%), sodium borohydride (98%), hydrogen peroxide (30%), polyvinylpyrrolidone (PVP), 2-mercaptoethanol (99%), 11-amino-1-undecanethiol hydrochloride (MUNH₂, 99%), ofloxacin powder (99%), orthovanadate (≥ 90%,), sodium chloride, sodium phosphate, sodium phosphate monobasic monohydrate, bacto-tryptone, and bacto yeast extract were purchased from Sigma-Aldrich. N-hydroxysulfosuccinimide (Sulfo-NHS, 98.5%, Pierce), 1-Ethyl-3-[3-dimethylaminopropyl]-carbodiimide hydrochloride (EDC, 99%, Pierce), silver perchlorate monohydrate (99%, Alfa Aesar), Live/dead backlight viability assay (Life Technologies), and Hoechst 33342 (Life Technologies) were purchased as indicated, and used as received. Cell line of Escherichia coli (wt w3110) was purchased from Genetic Stock Center (CGSC). Deionized (DI) water (18 MΩ water, Barnstead) was used to prepare all solutions including standard LB medium (1% tryptone peptone, 0.5% yeast extract, and 0.5% NaCl, pH = 7.2) and modified LB medium (1% tryptone peptone, 0.5% yeast extract, and 0.1% NaCl, pH = 7.2).

Songkiatisak P., Ding F., Cherukuri P.K, & Xu X.H. (2020). Size-dependent inhibitory effects of antibiotic nanocarriers on filamentation of E. coli. Nanoscale Advances, 2(5), 2135-2145.

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