Anti mouse cd16 32 clone 2.4g2

Manufactured by BD

Sourced in United States

Anti-mouse CD16/32 (clone 2.4G2) is a laboratory reagent used for the detection and analysis of mouse CD16/32 proteins. It is a monoclonal antibody that specifically binds to the mouse CD16/32 cell surface receptor, which is expressed on various immune cells such as macrophages, dendritic cells, and natural killer cells.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using anti mouse cd16 32 clone 2.4g2

Murine Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-mouse CD16/32 (clone 2.4G2, BD Pharmingen, BD, New Jersey, U.S.), anti-Ly6G (clone 1A8, BD Pharmingen, BD, New Jersey, U.S.), anti-CD11b (clone M1/70, eBioscience, Affymetrix, California, U.S.). Viability Dye eFluor™ 780 (eBioscience, Affymetrix, California, U.S.) or DAPI (BioLegend, California, U.S.) were used to determine viable cells.

Pylaeva E., Bordbari S., Spyra I., Decker A.S., Häussler S., Vybornov V., Lang S, & Jablonska J. (2019). Detrimental Effect of Type I IFNs During Acute Lung Infection With Pseudomonas aeruginosa Is Mediated Through the Stimulation of Neutrophil NETosis. Frontiers in Immunology, 10, 2190.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cell suspensions (1 × 10⁶ cells/100 μl) were prepared with dead cells excluded by using Zombie Aqua Fixable Viability Kit (Biolegend, USA). After being washed, cells were resuspended in buffer (PBS containing 5% FBS) and were blocked in Fc block (Anti-mouse CD16/32, Clone 2.4G2, BD, USA) at 4°C for 30 min. Cells were then incubated with following various antibodies for 30 min at 4°C. anti-CD11b (clone M1/70, Biolegend, USA), anti-F4/80 (clone BM8, Biolegend, USA), anti-Ly-6G/Ly-6C (clone RB6-8C5, eBioscience, USA), anti-CD11c (clone N418, Biolegend, USA), anti-I-A/I-E (clone M5/114.15.2, Biolegend, USA); Rat IgG2b,ҝ Isotype Ctrl (Biolegend, USA); anti-CD3 (clone 17A2, Biolegend, USA), Anti-CD4 (clone RM4-5, BD, USA), anti-CD8α (clone 53–6.7, BD, USA), anti-CD44 (clone IM7, BD, USA); anti-CD62L (clone MEL-14, eBioscience, USA), anti-Ki67 (clone SolA15, eBioscience, USA). Ki67 staining was implemented using Foxp3/Transcription Factor Staining Buffer Set (eBioscience, USA) according to manufacturers’ instructions.

Wei S., Huang J., Liu Z., Wang M., Zhang B., Lian Z., Guo Y, & Han H. (2019). Differential immune responses of C57BL/6 mice to infection by Salmonella enterica serovar Typhimurium strain SL1344, CVCC541 and CMCC50115. Virulence, 10(1), 248-259.

+ Open protocol

+ Expand

Murine Immune Cell Profiling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pravastatin sodium was purchased from Sigma-Aldrich (St. Louis, MO, USA). BV421-conjugated anti-mouse Ly6C (clone AL-21), PE-conjugated anti-mouse CD11b (clone M1/70), APC-conjugated anti-mouse Ly6G (clone 1A8), BV605-conjugated anti-mouse CD115 (clone T38-320), and anti-mouse CD16/32 (clone 2.4G2) were purchased from BD Pharmingen (Franklin Lakes, NJ, USA). Monoclonal antibodies against mouse F4/80 were obtained from Abcam (Cambridge, UK). Mounting medium with DAPI, hematoxylin–eosin (H&E) and Masson’s trichrome staining reagents were purchased from Wuhan Servicebio Technology (Wuhan, China). Oil Red O (ORO) was obtained from Sangon Biotech (Shanghai, China).

Chen Y., Zhang H., Hu L., Shi H., Liu X., Jia J., Sun S., Ou Y., Luo X., Zhou G, & Shen W. (2020). Pravastatin attenuates atherosclerosis after myocardial infarction by inhibiting inflammatory Ly6Chigh monocytosis in apolipoprotein E knockout mice. The Journal of International Medical Research, 48(7), 0300060520932816.

+ Open protocol

+ Expand

Monoclonal Antibodies Expression and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Monoclonal antibodies (mAbs) against Ebola glycoprotein (mAb114, c13C6, ADI-15742, KZ52 and ADI-16061), SARS-CoV-2 spike (COVA2-15, CB6 and CR3022), or influenza hemagglutinin (CH65, H2897, 6649, MEDI8852, CR9114, FI6v3, 8F8 and 8M2) were expressed in Expi-293F cells via transient transfection.
Mouse anti-His Tag antibody (clone J099B12, BioLegend #652502), goat anti-mouse IgG, HRP conjugated (clone Poly4053, BioLegend #405306), goat anti-mouse IgG1-HRP (SouthernBiotech #1070-05), goat anti-mouse IgG2a-HRP (SouthernBiotech #1083-05), goat anti-mouse IgG2b-HRP (SouthernBiotech #1093-05), or rabbit anti-human IgG, HRP conjugated (Abcam #ab6759) were used as detecting antibodies for Western-blot analysis or enzyme-linked immunosorbent assays (ELISAs).
For the analysis of germinal center responses, we stained cells with Ghost Dye Violet 510 (Tonbo Biosciences), anti-mouse CD16/32 (clone 2.4G2, BD Biosciences #553142), CD3 (clone 17A2, BioLegend #100216), CD4 (clone GK1.5, BioLegend #100469), CXCR5 (clone L138D7, BioLegend #145529), PD1 (clone 29F.1A12, BioLegend #135228), CD19 (clone 6D5, BioLegend #115534), CD95 (clone Jo2, BD Biosciences #557653) and CD38 (clone 90, BD Biosciences #740245).

Xu D., Li C., Utz A., Weidenbacher P.A., Tang S., Sanyal M., Pulendran B, & Kim P.S. (2022). Designing epitope-focused vaccines via antigen reorientation. bioRxiv.

+ Open protocol

+ Expand

Spleen Cell Phenotyping for Immune Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

At week 4 post-challenge infection, mice were euthanized to collect spleen cell samples. For cell phenotype analysis, cells from spleen (n=5) were stained with fluorophore-labeled surface markers. Anti-mouse CD16/32 (clone 2.4G2, BD Pharmingen, San Diego, California, USA) was used as Fc receptor blocker. Afterwards, antibody cocktails which contained anti-mouse CD23-FITC (clone 53-6.7, BD Pharmingen) and anti-mouse IgM-PerCP-Cy^™5.5 (clone R6-60.2, BD Pharmingen) were used to treat cells. The stained cells washed after incubation, acquired by flow cytometer LSRFortessa (BD Biosciences, San Diego, California, USA) and analyzed using FlowJo program (Tree Star Inc., San Carlos, California, USA).

Chu K.B., Kim S.S., Lee S.H., Lee D.H., Kim A.R, & Quan F.S. (2016). Immune Correlates of Resistance to Trichinella spiralis Reinfection in Mice. The Korean Journal of Parasitology, 54(5), 637-643.

+ Open protocol

+ Expand

Multicolor Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

For staining of cell surface molecules, cells were incubated with anti–mouse CD16/32 (clone 2.4G2; affinity purified from hybridoma culture supernatant) before labeling with cocktails of fluorescent antibodies specific for: CD11c (HL3), CD86 (GL1), MHCII (M5/114), CD326 (G8.8), CD4 (RM4-5), CD3 (145-2C11), and CD44 (IM7; all from BD); CD11b (M1/70), CD25 (7D4), Ly6A/E (D7), CD45 (30-F11), CD90.2 (53.2.1), CD2 (RM2-5), NK1.1 (PK136; BD); Ly6C (HK1.4), CD317 (BST2, PDCA-1; clone 927), CD273 (PDL2, Ty25), CD69 (H1.2F3; all from BioLegend); CD274 (PDL1, MIH5) and B220 (RA3-6B2; both from eBioscience). For intracellular cytokine staining, cells were surface stained, and then fixed and permeabilized using the Cytofix/Cytoperm kit (BD), and labeled with anti–IL-4 (11B11), anti–IFN-γ (XMG1.2), or respective IgG1 isotype controls (all from BD). Anti–IL-17A (eBio17B7) was obtained from eBioscience. Dead cells and doublets were identified and excluded from analysis using DAPI labeling or LIVE/DEAD Fixable Blue dead cell stain kit (both from Molecular Probes). Compensation was set in each experiment using CompBeads (BD). All samples were collected on a LSRII SORP flow cytometer (BD) and analyzed using FlowJo version 9.6.2 (Tree Star).

Connor L.M., Tang S.C., Cognard E., Ochiai S., Hilligan K.L., Old S.I., Pellefigues C., White R.F., Patel D., Smith A.A., Eccles D.A., Lamiable O., McConnell M.J, & Ronchese F. (2017). Th2 responses are primed by skin dendritic cells with distinct transcriptional profiles. The Journal of Experimental Medicine, 214(1), 125-142.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!