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Rabbit anti p53

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The Rabbit anti-p53 is a primary antibody directed against the p53 tumor suppressor protein. It is used for the detection and analysis of p53 in various research applications.

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Lab products found in correlation

Goat anti e cadherin, by R&D Systems (2 mentions) Mouse anti cdx2, by BioGenex (2 mentions) Rabbit anti vimentin, by Abcam (2 mentions) Mouse anti β catenin, by BD (2 mentions) Abc and dab kits, by Vector Laboratories (1 mentions) Goat anti cpa1, by R&D Systems (1 mentions) Rabbit anti ck19, by Abcam (1 mentions) Goat anti amylase, by Santa Cruz Biotechnology (1 mentions) Rabbit anti gfp, by Santa Cruz Biotechnology (1 mentions) Antigen unmasking solution, by Vector Laboratories (1 mentions) Mouse anti ki67, by BD (1 mentions) Aldosterone, by Merck Group (1 mentions) Goat anti nephrin, by R&D Systems (1 mentions) Rabbit anti wt1, by Santa Cruz Biotechnology (1 mentions) Fr167653, by Astellas Pharma (1 mentions) Mouse anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by Santa Cruz Biotechnology (1 mentions) Rabbit anti podocin, by Merck Group (1 mentions) Rabbit anti phospho p38 mapk, by Cell Signaling Technology (1 mentions)

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Anti-p53 (5 citations)

4 protocols using rabbit anti p53

1

Quantification of Pancreatic ADM and AFLs

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For quantification of ADM and AFLs, random whole pancreatic sections stained with hematoxylin and eosin H&E were analyzed. For immunohistochemical staining, sections of paraffin embedded pancreata were rehydrated and antigen retrieval was performed using Antigen Unmasking Solution (Vector Laboratories). Overnight incubation with the following primary antibodies was done at 4°C: goat anti-CPA1 (R&D), rabbit anti-CK19 (Abcam), rabbit anti-GFP (Santa Cruz Biotechnology), mouse anti-Ki67 (BD), rabbit anti-P53 (Vector Laboratories), goat anti-Amylase (Santa Cruz Biotechnology), rabbit anti-p44/p42 MAPK (Cell Signaling) and FITC-conjugated DBA-lectin (Vector Laboratories). Biotin-conjugated secondary antibodies were incubated for 1 h at room temperature, following development with ABC and DAB kits (both Vector Laboratories). Nuclear counterstaining was performed using haematoxylin. For immunofluorescence, sections were incubated with fluorophore-conjugated secondary antibody for 1 h at room temperature. Slides were mounted with DAPI hardset antifade mounting medium.
von Figura G., Fahrenkrog-Petersen L., Hidalgo-Sastre A., Hartmann D., Hüser N., Schmid R.M., Hebrok M., Roy N, & Esposito I. (2017). Atypical flat lesions derive from pancreatic acinar cells. Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 17(3), 350-353.
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2

Immunohistochemical Analysis of Mouse Tissue

Cited 1 time
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Mouse tissues were fixed in 10% (v/v) buffered formalin and embedded in paraffin. Sections of the paraffin-embedded tissues were subjected to immunohistochemical (IHC) analysis as described[8 (link)]. The following primary antibodies were used for IHC analysis: mouse anti–β-catenin (1:800; BD Biosciences, Lexington, KY), rabbit anti-p53 (1:500; Vector Laboratories Inc., Burlingame, CA), goat anti–E-cadherin (1:500; R&D Systems, Minneapolis, MN), Rabbit anti-vimentin (1:500; Epitomics, Burlingame, CA), and mouse anti-CDX2 (1:100; BioGenex, Fremont, CA). The IHC staining with anti-p53 antibody was classified according to the percentage of stained cells. Expression of p53 was considered to be weak if <10% of neoplastic cells were observed to be stained, and strong if >10% of neoplastic cells were stained.
Tang J., Feng Y., Kuick R., Green M., Green M., Sakamoto N., Kurosu Y., Lin J., Cho K.R, & Fearon E.R. (2019). Trp53 null and R270H mutant alleles have comparable effects in regulating invasion, metastasis and gene expression in mouse colon tumorigenesis. Laboratory investigation; a journal of technical methods and pathology, 99(10), 1454-1469.
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3

Podocyte Stress Signaling Pathways

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Aldosterone was obtained from Sigma Aldrich (St. Louis, MO). FR167653, p38α MAP Kinase inhibitor, was kindly provided by Astellas Pharma Inc. (Tokyo, Japan). Primary antibodies used for immunohistochemical studies and Western blotting were goat anti-nephrin (R&D Systems, Minneapolis, MN), rabbit anti-podocin (Sigma Aldrich), rabbit anti-phospho-p38 MAPK (Cell Signaling Technology, Boston, MA), rabbit total p38 MAPK (Cell Signaling Technology), rabbit anti-WT1 (Santa Cruz, Dallas, TX), and rabbit anti-p53 (Vector Laboratories, Burlingame, CA), rabbit anti-phospho-MKP-1 (Cell Signaling Technology), rabbit anti-phospho-MKK3 (Cell Signaling Technology), and mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Santa Cruz) antibodies. pRc/RSV Flag MKK350 (link) and pRc/RSV Flag MKK3 (glu)51 (link) were gifts from Professor Roger Davis (Addgene plasmid #14671 and #14670, respectively).
Kato Y., Mori K., Kasahara M., Osaki K., Ishii A., Mori K.P., Toda N., Ohno S., Kuwabara T., Tokudome T., Kishimoto I., Saleem M.A., Matsusaka T., Nakao K., Mukoyama M., Yanagita M, & Yokoi H. (2017). Natriuretic peptide receptor guanylyl cyclase-A pathway counteracts glomerular injury evoked by aldosterone through p38 mitogen-activated protein kinase inhibition. Scientific Reports, 7, 46624.
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4

Immunohistochemical Analysis of Mouse Tissue

Cited 1 time
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Mouse tissues were fixed in 10% (v/v) buffered formalin and embedded in paraffin. Sections of the paraffin-embedded tissues were subjected to immunohistochemical (IHC) analysis as described[8 (link)]. The following primary antibodies were used for IHC analysis: mouse anti–β-catenin (1:800; BD Biosciences, Lexington, KY), rabbit anti-p53 (1:500; Vector Laboratories Inc., Burlingame, CA), goat anti–E-cadherin (1:500; R&D Systems, Minneapolis, MN), Rabbit anti-vimentin (1:500; Epitomics, Burlingame, CA), and mouse anti-CDX2 (1:100; BioGenex, Fremont, CA). The IHC staining with anti-p53 antibody was classified according to the percentage of stained cells. Expression of p53 was considered to be weak if <10% of neoplastic cells were observed to be stained, and strong if >10% of neoplastic cells were stained.
Tang J., Feng Y., Kuick R., Green M., Green M., Sakamoto N., Kurosu Y., Lin J., Cho K.R, & Fearon E.R. (2019). Trp53 null and R270H mutant alleles have comparable effects in regulating invasion, metastasis and gene expression in mouse colon tumorigenesis. Laboratory investigation; a journal of technical methods and pathology, 99(10), 1454-1469.
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