Axioplan 2

Manufactured by Nikon

Sourced in United States

The Axioplan 2 is a high-performance microscope system designed for advanced imaging and analysis in research and industrial applications. It features a modular design, allowing for customization to suit a variety of needs. The core function of the Axioplan 2 is to provide a versatile and reliable platform for microscopic examination and documentation.

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Lab products found in correlation

Dxm1200, by Nikon (2 mentions) Axioplan 2, by Zeiss (1 mentions) Fluoview 1000, by Zeiss (1 mentions) Orca flash 4.0 scmos camera, by Hamamatsu Photonics (1 mentions) Dmx1200, by Nikon (1 mentions) Neutral buffered formalin, by Merck Group (1 mentions) Smz1500, by Nikon (1 mentions) Axio microscope, by Zeiss (1 mentions) Axiocam erc5, by Zeiss (1 mentions) Axio scan z1, by Zeiss (1 mentions) Ab25989, by Abcam (1 mentions) Hoechst nucleic acid stain, by Thermo Fisher Scientific (1 mentions) A1r gaasp, by Nikon (1 mentions) Alexa fluor 488 secondary antibody, by Thermo Fisher Scientific (1 mentions) Eclipse ti, by Nikon (1 mentions)

8 protocols using axioplan 2

Imaging Cilia Dynamics in Zebrafish

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Immunofluorescence images were captured on an Olympus Fluoview 1000 or a Zeiss Apotome Axiovert Z1 microscope with 60×, 1.4 NA oil immersion objectives. Bright-field images were captured with a Zeiss Axioplan 2 compound microscope using a Nikon DMX1200 digital camera. Live imaging of cilia were performed in Tg(actinb2::Arl13b-GFP) embryos or in embryos injected with 188 pg of RNA encoding Arl13b-GFP (Borovina et al., 2010 (link)) using a Zeiss Axioplan 2 compound microscope fitted with either an Evolve 512 EMCCD camera (Photometrics) or an Orca Flash 4.0 sCMOS camera (Hamamatsu). Images were processed in Adobe Photoshop. Cilia length measurements and movie editing were performed using ImageJ. Figures were assembled using Adobe Illustrator.

Choksi S.P., Babu D., Lau D., Yu X, & Roy S. (2014). Systematic discovery of novel ciliary genes through functional genomics in the zebrafish. Development (Cambridge, England), 141(17), 3410-3419.

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Histological Analysis of Brain Tumour Samples

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Brain tumour samples were fixed in 10% neutral buffered formalin (Sigma) and embedded in paraffin. The brain tumour samples were sectioned at 5 μm, were stained with haematoxylin and eosin (H&E) at the Harvard University Rodent Histopathology Core Facility (Boston, MA), and were examined by light microscopy using either a Zeiss Axioplan 2 or Nikon SMZ1500 light microscope. Images were acquired using SPOT Imaging Solutions (Diagnostic Instruments, Inc.) cameras and software. All histological sections were evaluated at the Harvard University Rodent Histopathology Core Facility.

Mukherjee P., Augur Z.M., Li M., Hill C., Greenwood B., Domin M.A., Kondakci G., Narain N.R., Kiebish M.A., Bronson R.T., Arismendi-Morillo G., Chinopoulos C, & Seyfried T.N. (2019). Therapeutic benefit of combining calorie-restricted ketogenic diet and glutamine targeting in late-stage experimental glioblastoma. Communications Biology, 2, 200.

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RNAi Clones and Worm Imaging

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Sequence-verified RNAi clones used in this work were obtained from the Ahringer library (Kamath and Ahringer 2003 (link)) and the Vidal library (Rual et al. 2004a) (gifts from Dr. Iqbal Hamza of UMCP and Dr. Mark Wilson of JHU). RNAi on L1 and P0 animals was performed as described (Kamat et al. 2001 (link); Liu et al. 2014 (link)). Worms of the appropriate age were analyzed on a Zeiss Axioplan 2 equipped with Nikon DXM1200 digital camera for imaging.

Gorrepati L., Krause M.W., Chen W., Brodigan T.M., Correa-Mendez M, & Eisenmann D.M. (2015). Identification of Wnt Pathway Target Genes Regulating the Division and Differentiation of Larval Seam Cells and Vulval Precursor Cells in Caenorhabditis elegans. G3: Genes|Genomes|Genetics, 5(8), 1551-1566.

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Whole Mount X-Gal and Immunostaining Protocols

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Whole mount X-gal staining was carried out as described before (Mongan et al., 2008 (link)). For histology and immunohistochemistry, embryos’ heads were fixed in 4% paraformaldehyde at 4°C overnight. Tissues were embedded in either paraffin or OCT and frozen. Tissue sections were processed using standard protocols, followed by either Hematoxylin/Eosin or immunostaining. Transfected HeLa cells on 12-mm glass cover slips were fixed with 4% formaldehyde, followed by permeablization and immunostaining. Immunostaining was done by using specific antibodies plus Alexa Fluor dye-conjugated secondary antibodies, Alexa Fluor dyeconjugated Phalloidin to label F-actin, and Hoechst to stain nuclei. Images were captured using a Leica MZ16 FA dissecting microscope, Zeiss Axio microscope equipped with an AxioCam ERc5s and Zeiss Axioplan 2 imaging fluorescence microscope, or Nikon A1R si inverted single photon laser scanning microscope.

Meng Q., Mongan M., Wang J., Tang X., Zhang J., Kao W, & Xia Y. (2014). Epithelial sheet movement requires the cooperation of c-Jun and MAP3K1. Developmental biology, 395(1), 29-37.

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Immunostaining of C. elegans Proteins

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Immunostaining of worms was performed as described (Duerr 2006 (link)). Anti-FLAG mouse monoclonal antibody M2 (Sigma #F3165) was used at 1/1000 for PAB-1 detection and MH27 mouse monoclonal antibody (Developmental Studies Hybridoma Bank) at 1/100 for detection of the junctional molecule AJM-1. Stained worms were mounted and were analyzed on a Zeiss Axioplan 2 equipped with a Nikon DXM1200 digital camera for imaging.

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Histological Analysis of Human Embryos

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Embryos used for this study are historical specimens and belong either to the Carnegie collection of the Developmental Anatomy Center at the National Museum of Health and Medicine in Silver Spring, MD, USA, or to the Human Embryology Collection (Blechschmidt Collection) of the Centre of Anatomy, University Medical Centre Göttingen, Göttingen, Germany. Images of embryos #8943 and #836 from the Carnegie collection (Gasser et al., 2017) are available at the open source website http://virtualhumanembryo.lsuhsc.edu/. Embryos had been fixed, embedded, sectioned, and histologically stained using different protocols. Details about stage, fixation, embedding can be found in Table 1. Images were acquired using Zeiss Axio Scan.Z1, Zeiss Axioplan 2, or Nikon E800 microscopes and processed as described (de Bakker et al., 2016; Rulle et al., 2018).

Schäfer T., Stankova V., Viebahn C., de Bakker B, & Tsikolia N. (2020). Initial morphological symmetry breaking in the foregut and development of the omental bursa in human embryos. Journal of Anatomy, 238(4), 1010-1022.

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Neutrophil and NET Visualization

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Neutrophil and NET images were captured on a Zeiss Axioplan 2 or a Nikon A1R GaAsP inverted SP microscope using antibodies to NE (481001, Millipore Sigma, Burlington, MA, USA) and MPO (ab25989, Abcam, Cambridge, UK) with AF568 and AF488 secondary antibodies (Invitrogen, Carlsbad, CA, USA) and Hoechst nucleic acid stain (Invitrogen).

Collins M.S., Imbrogno M.A., Kopras E.J., Howard J.A., Zhang N., Kramer E.L, & Hudock K.M. (2023). Heterogeneity in Neutrophil Extracellular Traps from Healthy Human Subjects. International Journal of Molecular Sciences, 25(1), 525.

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Skin Spindle Orientation Analysis

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Neonatal, adult, and aged human skin samples were formalin‐fixed and paraffin‐embedded. Five to 8 micrometer sections were incubated with anti‐α tubulin (clone TU‐01,13‐8000, Invitrogen) and anti‐γtubulin (clone GTU‐88, ab11316, Abcam) (microtubules and centrioles, respectively) antibodies and AlexaFluor 488 secondary antibodies (Invitrogen), to identify spindle orientation of cell divisions. DAPI was used to identify nuclei. Fluorescence was detected using a Zeiss Axioplan 2 microscope or a confocal microscope (Nikon Eclipse Ti). The angle between the spindle axis and the basal layer (angle of division) was measured using the angle tool from ImageJ^® software. Divisions with angles less than 30° were considered parallel and divisions with angles between 60° and 90° were considered perpendicular (Charruyer et al., 2017; Lechler & Fuchs, 2005). Basal layer epidermal length was measured using ImageJ^®.

Charruyer A., Weisenberger T., Li H., Khalifa A., Schroeder A.W., Belzer A, & Ghadially R. (2021). Decreased p53 is associated with a decline in asymmetric stem cell self‐renewal in aged human epidermis. Aging Cell, 20(2), e13310.

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