Anti rabbit igg h l hrp conjugate 170 6515

Manufactured by Bio-Rad

Sourced in United States

The Anti-rabbit IgG (H + L)-HRP conjugate (170-6515) is a laboratory reagent used for detection and quantification applications. It consists of horseradish peroxidase (HRP) conjugated to anti-rabbit immunoglobulin G (IgG) antibodies that recognize the heavy and light chains of rabbit IgG. This product can be utilized in various immunoassay techniques that involve rabbit antibodies.

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Lab products found in correlation

Goat anti mouse 170 6516, by Bio-Rad (2 mentions) Mouse monoclonal anti gapdh gtx3066, by GeneTex (2 mentions)

2 protocols using anti rabbit igg h l hrp conjugate 170 6515

Immunoblotting and Immunofluorescence Protocols for MCL-1, BCL-2, and BCL-XL

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The following primary and secondary antibodies were used for immunoblotting: mouse monoclonal anti-MCL-1 (clone 10, sc-12756) purchased from Santa Cruz Biotechnology, Inc., Dallas, TX, USA; rabbit monoclonal anti-BCL-2 (clone 50E3, #2870), rabbit monoclonal anti-BCL-XL (clone 54H6, #2764) purchased from Cell Signaling Technology, Inc., Danvers MA, USA; and mouse monoclonal anti-GAPDH (GTX3066) purchased from GeneTex, Inc., Irvine, CA, USA; goat anti-mouse (170-6516) or anti-rabbit IgG (H + L)-HRP conjugate (170-6515) purchased from BioRad, Hercules, CA, USA. For indirect immunofluorescence, mouse monoclonal anti-MCL-1 (clone 10, sc-12756) purchased from Santa Cruz Biotechnology, Inc., Dallas, TX, USA and Alexa Fluor 568 goat anti-mouse (A-11031) secondary antibody obtained from Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA, were used.

Rysanek D., Vasicova P., Kolla J.N., Sedlak D., Andera L., Bartek J, & Hodny Z. (2022). Synergism of BCL-2 family inhibitors facilitates selective elimination of senescent cells. Aging (Albany NY), 14(16), 6381-6414.

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Immunoblotting Antibody Detection Protocol

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For immunoblotting, the following primary and secondary antibodies were used: rabbit polyclonal p70 S6 kinase (#9202), rabbit monoclonal phospho-p70 S6 kinase (Thr389) (#9234), rabbit monoclonal AKT kinase (#4691), rabbit monoclonal phospho-AKT kinase (Ser473) (#9234) obtained from Cell Signaling Technology, Inc., Danvers MA, USA; p21waf1 (sc-56335) obtained from Santa Cruz Biotechnology, Inc., Dallas, TX, USA and mouse monoclonal anti-GAPDH (GTX3066) purchased from GeneTex, Inc., Irvine, CA, USA; goat anti-mouse (170-6516) or anti-rabbit IgG (H + L)-HRP conjugate (170-6515) purchased from BioRad, Hercules, CA, USA.

Chrienova Z., Rysanek D., Oleksak P., Stary D., Bajda M., Reinis M., Mikyskova R., Novotny O., Andrys R., Skarka A., Vasicova P., Novak J., Valis M., Kuca K., Hodny Z, & Nepovimova E. (2022). Discovery of small molecule mechanistic target of rapamycin inhibitors as anti-aging and anti-cancer therapeutics. Frontiers in Aging Neuroscience, 14, 1048260.

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