Cells were washed in phosphate-buffered saline (PBS) and harvested with Accutase (EMD Millipore, #SCR005) for 10 min at room temperature. Dissociated cells were washed once in flow staining buffer (PBS + 1% FBS) and incubated with respective flow antibodies at 4 °c for 20 min in the dark. Flow cytometry was performed using the following antibodies: H2Kq/AF647 (Biolegend clone KH114, 1:200), PD-L1/PE (BioLegend Clone 10F.9G2, 1:100), H2Kb/AF488 (BioLegend Clone AF6-88.5, 1:400), H2Kb-SIINFEKL (BioLegend Clone 25-D1.16, 1:200), H2Kd/PE (BioLegend Clone SF1-1.1, 1:400). DAPI was used as a viability dye for dead cell exclusion. Samples were analyzed on an Attune NxT flow cytometer (Life Technologies).
H2kb siinfekl
Manufactured by BioLegend
H2Kb-SIINFEKL is a major histocompatibility complex (MHC) class I molecule that presents the SIINFEKL peptide derived from the chicken ovalbumin protein. This product is commonly used in research applications involving antigen presentation and T cell activation.
Automatically generated - may contain errors
2 protocols using h2kb siinfekl
1
Multiparametric Flow Cytometry Analysis
2
Flow Cytometric Analysis of MHC Class I Molecules
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Cells were washed in phosphate-buffered saline (PBS) and harvested with Accutase (EMD Millipore, #SCR005) for 10 min at room temperature. Dissociated cells were washed once in flow staining buffer (PBS + 1% FBS) and incubated with respective flow antibodies at 4 °C for 20 min in the dark. Flow cytometry was performed using the following antibodies: H2Kq/AF647 (Biolegend clone KH114, 1:200), PD-L1/PE (BioLegend Clone 10F.9G2, 1:100), H2Kb/AF488 (BioLegend Clone AF6-88.5, 1:400), H2Kb-SIINFEKL (BioLegend Clone 25-D1.16, 1:200), H2Kd/PE (BioLegend Clone SF1-1.1, 1:400). DAPI was used as a viability dye for dead cell exclusion. Samples were analyzed on an Attune NxT flow cytometer (Life Technologies).
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