Gemini xps fluorometer

Bbss DNA was isolated from the cultured isolates with either the IsoQuick kit (Orca Research, Bothell, WA), the Gentra PureGene DNA Isolation Kit (Qiagen Inc., Valencia, CA), or the DNEasy kit (Qiagen Inc, Valencia, CA). Short-read next-generation sequencing (NGS) library construction was performed using the Nextera XT Library Prep Kit (Illumina, San Diego, CA). DNA quantification was performed in a 96-well microplate using the SpectraMax Quant dsDNA Assay Kit and the Gemini XPS Fluorometer (Molecular Devices, San Jose, CA), or in a single tube using the Qubit 2.0 fluorometer (Thermo Fisher Scientific, Springfield Township, NJ). Library quality was examined using the 4200 TapeStation and D1000 ScreenTape (Agilent, Santa Clara, CA). Paired-end sequencing (2 × 150 or 250 cycles) was performed using the NextSeq 550 or MiSeq system (Illumina).

Bb DNA was isolated from the cultured isolates with either the IsoQuick kit (Orca Research, Bothell, WA), the Gentra PureGene DNA Isolation Kit (Qiagen Inc., Valencia, CA), or the DNEasy kit (Qiagen Inc, Valencia, CA). Short-read next-generation sequencing (NGS) library construction was performed using the Nextera XT Library Prep Kit (Illumina, San Diego, CA). DNA quantification was performed in a 96-well microplate using the SpectraMax Quant dsDNA Assay Kit and the Gemini XPS Fluorometer (Molecular Devices, San Jose, CA), or in a single tube using the Qubit 2.0 fluorometer (Thermo Fisher Scientific, Springfield Township, NJ). Library quality was examined using the 4200 TapeStation and D1000 ScreenTape (Agilent, Santa Clara, CA). Paired-end sequencing (2 × 150 or 250 cycles) was performed using the NextSeq 550 or MiSeq system (Illumina).

DNA was extracted from bacterial isolates using the Agencourt GenFind DNA Isolation Kit and the Biomek FX^P Automated Workstation (Beckman Coulter). A short-read NGS library was prepared using the Nextera DNA Flex or XT Library Prep Kit (Illumina, San Diego, CA, USA). DNA quantification was performed in a microplate using the SpectraMax Quant dsDNA Assay Kit and the Gemini XPS Fluorometer (Molecular Devices, San Jose, CA, USA), or in a single tube using the Qubit 2.0 fluorometer (Thermo Fisher Scientific, Springfield Township, NJ, USA). Library quality was examined using the 4200 TapeStation and D1000 ScreenTape (Agilent, Santa Clara, CA, USA). Paired-end sequencing (2 × 150 cycles) was performed using the NextSeq 550 system (Illumina). The Pacific Biosciences (PacBio, Menlo Park, CA, USA) RSII single-molecule real-time (SMRT) sequencing system was employed for the long-read sequencing of A. baumannii PB364. The long-read NGS library was processed using g-TUBE fragmentation (Covaris, Woburn, MA, USA), BluePippin size selection (Sage Science, Beverly, MA, USA) and a SMRTbell template preparation kit (PacBio). Mainly, the manufacturers’ standard protocols were followed.