The following lipid standards were purchased from Cayman Chemical: 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic-6,8,9,11,12,14,15-d7 acid (5-oxoETE-d7, ≥99% deuterated product), 17-oxo-4Z,7Z,10Z,13Z,15E,19Z-docosahexaenoic acid (17-oxo-DHA, ≥98%), 17-oxo-7Z,10Z,13Z,15E,19Z-docosapentaenoic acid (17-oxo-DPA, ≥98%), (±)13-hydroxy-4Z,7Z,10Z,14E,16Z,19Z-docosahexaenoic acid (13-OH-DHA, >98%), (±)7-hydroxy-4Z,8E,10Z,13Z,16Z,19Z-docosahexaenoic acid (7-OH-DHA, >98%), 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-oxoETE, >95%), 5S-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-OH-ETE, >98%), (±)-5-hydroxy-6E,8Z,11Z,14Z,17Z-eicosapentaenoic acid (5-OH-EPA, >98%). Docosahexaenoic acid (DHA, >99%) and docosapentaenoic acid (DPA, >99%) were purchased from NuCheck Prep, Inc.
5 oxo 6e
Manufactured by Cayman Chemical
Sourced in Germany
5-oxo-6E is a research chemical compound commonly used in scientific laboratories. It serves as a precursor or intermediate in the synthesis of other compounds. The core function of 5-oxo-6E is to facilitate chemical reactions and transformations as part of the research and development process. No further details on its intended use are provided.
Automatically generated - may contain errors
Lab products found in correlation
Docosapentaenoic acid, by Cayman Chemical (1 mentions)
Eicosatetraenoic acid, by Cayman Chemical (1 mentions)
Eicosapentaenoic acid, by Cayman Chemical (1 mentions)
Docosahexaenoic acid, by Cayman Chemical (1 mentions)
Glutathione colorimetric detection kit, by Arbor Assays (1 mentions)
Biocoll, by Harvard Bioscience (1 mentions)
Z eicosatetraenoic acid, by Cayman Chemical (1 mentions)
Jc 1 staining kit, by Merck Group (1 mentions)
2 protocols using 5 oxo 6e
1
Lipid Standards for Targeted Lipidomics
2
Neutrophil Biochemical Assays Protocol
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The chemicals were obtained from the following sources: aminophenyl fluorescein (APF) and HPLC-standards 5S-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-HpETE), 5Shydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-HETE), 5S,12R-dihydroxy-6E,8E,10E,14Zeicosatetraenoic acid (5,12-DiHETE), 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-oxo-ETE), LTB4, prostaglandin B2 (PGB2) and AA were from Cayman Chemical (distributed by Biomol, Hamburg, Germany); HPLC solvents were from Carl Roth (Karlsruhe, Germany); glutathione colorimetric detection kit from Arbor Assays (Ann Arbor, Michigan, United States); biocoll (10 mM HEPES, 1.077 g/l, isotone) from Biochrom (Berlin, Germany) and all other chemicals including the JC-1 staining kit (CS0390) were from Sigma (Taufkirchen, Germany).
Working solutions of H2O2 were prepared by dilution of the corresponding stock solutions. Their concentrations were determined spectrophotometrically using ε240 = 43.6 M -1 cm -1 [Beers and Sizer 1952 ]. The buffer system hanks' balanced salt solution (HBSS) with and without Ca 2+ for the resuspension of neutrophils was prepared daily and adjusted to pH 7.4.
Working solutions of H2O2 were prepared by dilution of the corresponding stock solutions. Their concentrations were determined spectrophotometrically using ε240 = 43.6 M -1 cm -1 [Beers and Sizer 1952 ]. The buffer system hanks' balanced salt solution (HBSS) with and without Ca 2+ for the resuspension of neutrophils was prepared daily and adjusted to pH 7.4.
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