Log In Sign up
The largest database of trusted experimental protocols

Anti human hb egf

Manufactured by R&D Systems
Visit Supplier

Anti-human HB-EGF is a recombinant protein that binds to the epidermal growth factor receptor (EGFR) and inhibits the biological activities of HB-EGF (heparin-binding EGF-like growth factor).

Automatically generated - may contain errors

Lab products found in correlation

Anti egfp, by Thermo Fisher Scientific (4 mentions) Anti human neuronal protein huc hud, by Thermo Fisher Scientific (2 mentions) Anti ki67, by Abcam (2 mentions) Lsm 700 confocal microscope, by Zeiss (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (2 mentions)

Spelling variants of this product

HB-EGF (34 citations) Human EGF (24 citations) Human HB-EGF (4 citations) Anti-HB-EGF (3 citations) Anti-EGF (3 citations)

2 protocols using anti human hb egf

1

Multicolor Immunofluorescence Analysis of Murine Intestine

Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine intestinal tissue sections (4μm thickness) were double-stained with anti-Ki67 (1:400; Abcam, Cambridge, MA) and anti-EGFP (1:500; Thermo, Rockford, IL) antibodies, or with anti-human HB-EGF (5μg/ml;R&D system, Minneapolis, MN) and anti-EGFP (1:500) antibodies, or with anti-human neuronal protein HuC/HuD (HuC/D; a pan neuronal cell marker) (5 μg/ml; Life Technology, Eugene, OR) and anti-EGFP (1:500) antibodies, followed by species-specific secondary antibodies conjugated to Cy3 or fluorescein isothiocyanate, respectively. Coverslips were mounted onto glass slides with mounting medium containing DAPI (Vector, Burlingame, CA). Images were visualized using a Zeiss LSM 700 confocal microscope (Zeiss, Thornwood, NY) with a 40x objective. Five serial histologic sections per pup were examined.
Wei J., Zhou Y, & Besner G.E. (2015). Heparin-Binding EGF-like Growth Factor and Enteric Neural Stem Cell Transplantation in the Prevention of Experimental Necrotizing Enterocolitis. Pediatric research, 78(1), 29-37.
+ Open protocol
+ Expand
2

Multicolor Immunofluorescence Analysis of Murine Intestine

Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine intestinal tissue sections (4μm thickness) were double-stained with anti-Ki67 (1:400; Abcam, Cambridge, MA) and anti-EGFP (1:500; Thermo, Rockford, IL) antibodies, or with anti-human HB-EGF (5μg/ml;R&D system, Minneapolis, MN) and anti-EGFP (1:500) antibodies, or with anti-human neuronal protein HuC/HuD (HuC/D; a pan neuronal cell marker) (5 μg/ml; Life Technology, Eugene, OR) and anti-EGFP (1:500) antibodies, followed by species-specific secondary antibodies conjugated to Cy3 or fluorescein isothiocyanate, respectively. Coverslips were mounted onto glass slides with mounting medium containing DAPI (Vector, Burlingame, CA). Images were visualized using a Zeiss LSM 700 confocal microscope (Zeiss, Thornwood, NY) with a 40x objective. Five serial histologic sections per pup were examined.
Wei J., Zhou Y, & Besner G.E. (2015). Heparin-Binding EGF-like Growth Factor and Enteric Neural Stem Cell Transplantation in the Prevention of Experimental Necrotizing Enterocolitis. Pediatric research, 78(1), 29-37.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!