Anti-phosphorylated and pan, AKT, and MAPK antibodies, and PI3Kα/β/γ antibodies were purchased from Cell Signalling Technology (Cambridge, MA, USA). Anti-PI3Kδ antibody was purchased from R&D systems (Oxford, UK). Anti-CD138-PE, anti-CD90-FITC, anti-CD73-PE, anti-CD105-APC antibodies (Cat. 130-098-122, 130095403, 130095182, 130094926) and interleukin-6 (IL-6) were purchased from Miltenyi Biotec (Auburn, CA, USA). Idelalisib, CZC24832, duvelisib were obtained from Selleck Chemicals (Houston, TX, USA). All other reagents were obtained from Sigma-Aldrich (St Louis, MO, USA), unless otherwise indicated.
Czc24832
Manufactured by Selleck Chemicals
Sourced in United States, Mongolia
CZC24832 is a laboratory equipment item. It is a precision instrument used for scientific and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Tgx 221, by Selleck Chemicals (2 mentions)
Cal 101, by Selleck Chemicals (2 mentions)
Idelalisib, by Selleck Chemicals (1 mentions)
Duvelisib, by Selleck Chemicals (1 mentions)
Anti cd73 pe, by Miltenyi Biotec (1 mentions)
Anti phosphorylated and pan, by Cell Signaling Technology (1 mentions)
Anti pi3kδ antibody, by R&D Systems (1 mentions)
Anti cd138 pe, by Miltenyi Biotec (1 mentions)
Anti cd90 fitc, by Miltenyi Biotec (1 mentions)
Interleukin 6 il 6, by Miltenyi Biotec (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Huvecs, by Merck Group (1 mentions)
C12430500bt, by Thermo Fisher Scientific (1 mentions)
Dasatinib, by Selleck Chemicals (1 mentions)
Ipi 145, by Selleck Chemicals (1 mentions)
Byl719, by Selleck Chemicals (1 mentions)
Prt062607, by Selleck Chemicals (1 mentions)
6 well cell culture cluster, by Corning (1 mentions)
4 protocols using czc24832
1
Multiparametric Analysis of PI3K Signaling
2
HUVEC Cultivation and LPS Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human umbilical vein endothelial cells (HUVECs) were purchased from the Cell Bank of Shanghai Institute of Cell Biology, Chinese Academy of Sciences. Cells were cultured in Dulbecco's modified Eagle medium (C12430500BT, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco), 100-U/mL penicillin, and 100-mg/mL streptomycin (Gibco) in an incubator at 37°C with 5% CO2. HUVECs were treated with indicated concentrations of lipopolysaccharide (LPS) (L4391; Sigma-Aldrich Co. Ltd, MO, USA) for different times. To examine the role of PI3Kγ in LPS-treated endothelial cells, 2-μmol/L CZC24832 (S7018; Selleck Chemicals) was added to culture medium 2 h before the administration of LPS.
3
DLBCL Cell Line Culture and Drug Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The ABC DLBCL lines TMD8, HBL1, OYB, U2932, SUDHL2, and TK, and the GCB-DLBCL lines BJAB, OCI-Ly1, and OCI-Ly8 were grown in RPMI 1640 plus 20% FBS plus Pen/Strep. ABC DLBCL lines OCI-Ly10, OCI-Ly3 and DLBCL2 were grown in RPMI 1640 plus 20% heparinized human plasma plus Pen/Strep. Cell lines were previously engineered to express an ecotropic retroviral receptor and a bacterial tetracycline repressor [4 (link)]. All drugs (PRT062607, Dasatinib, BYL719, CAL-101, IPI-145, TGX221, CZC24832) were obtained from Selleckchem and diluted in DMSO.
4
Osteosarcoma Cell Line Responses to Wnt and PI3K Modulators
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human MG-63 and U2OS osteosarcoma cell lines were purchased from Cells Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China). These cells were cultured in Dulbecco-modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Hyclone, Logan, UT), at 37 °C in a humidified atmosphere with 5% CO2. MG-63 cells were plated onto 6-well cell culture clusters (Costar) and grown to 80% confluence, and then serum-starved for 24 h. These cells were subsequently treated with recombinant sfrp2 or Wnt5a (R&D Systems, Minneapolis, MN) or PI3K/Akt inhibitors (LY294002, HS-173, TGX-221, CZC24832, CAL-101, MK-2206, A-674563, CCT128930) (Selleck, Houston, TX) before small G-protein activation assays and cell migration assays.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!