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Tigit 1g9

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TIGIT (1G9) is a monoclonal antibody that recognizes the human T-cell immunoglobulin and ITIM domain (TIGIT) protein. TIGIT is an inhibitory receptor expressed on various immune cells, including T cells, NK cells, and regulatory T cells. The TIGIT (1G9) antibody can be used for flow cytometry and other immunoassays to detect and analyze TIGIT-expressing cells.

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Lab products found in correlation

Foxp3 staining buffer set, by Thermo Fisher Scientific (1 mentions) Fjk 16, by Thermo Fisher Scientific (1 mentions) Annexin 5 dead cell apoptosis kit, by Thermo Fisher Scientific (1 mentions) Live dead yellow dye, by Thermo Fisher Scientific (1 mentions) Cd3 145 2c11, by Thermo Fisher Scientific (1 mentions) Anti cd16 cd32 antibody, by BD (1 mentions) Foxp3 fjk 16s, by Thermo Fisher Scientific (1 mentions) Cd25 pc61, by BD (1 mentions) Cd8 53 6, by BD (1 mentions) Cd62l mel 14, by BD (1 mentions) F4 80 ci a3 1, by Bio-Rad (1 mentions) Cd44 im7, by BD (1 mentions) Cd11c hl3, by BD (1 mentions) Cd4 rm4 5, by BD (1 mentions) Cd11b m1 70, by BD (1 mentions) Ki67 16a8, by BioLegend (1 mentions) Klrg1 2f1, by BioLegend (1 mentions) B220 ra3 6b2, by BD (1 mentions) Cd49b dx5, by BioLegend (1 mentions) Adenosine 5 triphosphate disodium salt, by Merck Group (1 mentions)

Spelling variants of this product

Anti-TIGIT (clone 1G9, (3 citations) TIGIT-APC (1G9, (2 citations)

4 protocols using tigit 1g9

1

Multiparametric Flow Cytometry Assay

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For surface staining, fluorophore-conjugated mAbs specific for CD4 (RM4-5), CD19 (6D5), CD25 (PC61), CD11c (N418), CD11b (M1/70), Gr-1 (RB6-8C5), CD45 (30-F11), TCRβ (H57-597), PD-1 (RMP1-30), CD8α (53-6.7), CD3 (17A2), and TIGIT (1G9) were obtained from BioLegend. Abs recognizing CXCR5 (2G8) were from BD Pharmingen. Abs recognizing P2RX7 (Hano43) were from Novus Biologicals. For intranuclear staining, buffers from a Foxp3 Staining Buffer Set (eBioscience) were used to stain with Abs recognizing Bcl-6(K112-91, BD Pharmingen) and Foxp3 (FJK-16s, eBioscience). For the cell death assay, cells were stained with Live/Dead® Yellow Dye (LifeTechnologies) and then surface markers, and Annexin V binding accomplished using the Annexin V/Dead Cell Apoptosis Kit (LifeTechnologies) according to the manufacturer's instructions. Cells were run on an LSRII (BD Biosciences), and analyses were performed with FlowJo (TreeStar) software.
Felix K.M., Teng F., Bates N.A., Ma H., Jaimez I.A., Sleiman K.C., Tran N.L, & Wu H.J. (2019). P2RX7 Deletion in T Cells Promotes Autoimmune Arthritis by Unleashing the Tfh Cell Response. Frontiers in Immunology, 10, 411.
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2

Immune cell profiling in tumor samples

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Single-cell suspensions from tumors or splenocytes were incubated with anti-Fc receptor antibody (2.4G2) on ice for 15 minutes in FACS buffer (PBS with 1% FBS and sodium azide). The cells were then stained with the appropriate antibodies in 2.4G2-containing FACS buffer on ice for 30 minutes. For intracellular cytokine staining, cells were fixed and permeabilized in buffer and stained with antibodies to detect intracellular cytokines or transcription factors. All samples were acquired on LSRII flow cytometers and analyzed with Flowjo (Flowjo, LLC., Ashland, Or). Antibodies against CD45 (A20), CD8 (53-6.7) and CD3 (145-2C11) were purchased from eBioscience. The antibodies against CD4 (RM4-5), PD-1 (RMP1-14), and TIGIT (1G9) were purchased from Biolegend.
Wang J., Perry C.J., Meeth K., Thakral D., Damsky W., Micevic G., Kaech S., Blenman K, & Bosenberg M. (2017). UV-induced somatic mutations elicit a functional T cell response in the YUMMER1.7 Mouse Melanoma Model. Pigment cell & melanoma research, 30(4), 428-435.
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3

Multiparametric Flow Cytometry Analysis of Immune Cell Subsets

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Mouse spleen and PLN were mechanically dissociated into single cells with use of 40-μm filters, and red cell lysis was performed with ACK buffer. Blocking was performed with anti-CD16/CD32 antibodies (BD Biosciences), and cells were stained with antibodies against CD4 (RM4-5; BD Biosciences), CD8 (53-6.7; BD Biosciences), CD11b (M1/70; BD Biosciences), CD11c (HL3; BD Biosciences), B220 (RA3-6B2; BD Biosciences), F4/80 (CI:A3-1; Bio-Rad Laboratories), CD62L (MEL-14; BD Biosciences), CD44 (IM7; BD Biosciences), CD25 (PC61; BD Biosciences), FoxP3 (FJK-16s; eBioscience), TIGIT (1G9; BioLegend), KLRG1 (2F1; BioLegend) and Ki67 (16A8; BioLegend). Samples were analyzed on the LSRII (BD Biosciences) and FlowJo (Tree Star, Inc.).
Leung S.S., Borg D.J., McCarthy D.A., Boursalian T.E., Cracraft J., Zhuang A., Fotheringham A.K., Flemming N., Watkins T., Miles J.J., Groop P.H., Scheijen J.L., Schalkwijk C.G., Steptoe R.J., Radford K.J., Knip M, & Forbes J.M. (2022). Soluble RAGE Prevents Type 1 Diabetes Expanding Functional Regulatory T Cells. Diabetes, 71(9), 1994-2008.
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4

Murine immune cell phenotyping

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C57BL/6JRj wild-type mice obtained from Janvier Labs were used for all experiments. Mice were housed in a specific pathogen-free facility and were aged of 8 weeks at the beginning of experiments. All animal experimental protocols were approved by the French Ministry of Education and Research, after consultation of the ethical committee (n° APAFIS#27682). Adenosine 5’-tri-phosphate disodium salt was purchased from Sigma Aldrich (A2383). Red blood cell (RBC) lysis/fixation Solution, True-Nuclear Transcription factor buffer set, and antibodies to CD45 (clone 30-F11), CD4 (RM’-5), CD8 (53-6.7), CD25 (PC-61), CD19 (1D3/CD19), FoxP3 (MF-14), CD27 (LG.3A10), CD62L (MEL-14), P2X7R (1F11), CD39 (Duha59), CD73 (CXCR3-176), TIGIT (1G9), TIM3 (RMT3-23), CD49b (DX5), NK1.1 (PK136), CX3CR1 (SAO11F11), PD-1 (29F1A12), CD11c (N418), CD11b (M1170), CD44 (IM7), XCR1 (ZET), P2X7 (1F11) and purified CD16/D32 (TruStain FcX) were obtained from Biolegend or Sony Biotechnology.
Demeules M., Scarpitta A., Hardet R., Gondé H., Abad C., Blandin M., Menzel S., Duan Y., Rissiek B., Magnus T., Mann A.M., Koch-Nolte F, & Adriouch S. (2022). Evaluation of nanobody-based biologics targeting purinergic checkpoints in tumor models in vivo. Frontiers in Immunology, 13, 1012534.
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