The specimens were photographed using a Canon EOS 5D digital SLR camera equipped with a Canon 50 mm macro lens and cross-polarized lights. Close-ups were captured by using a Leica DFC 500 digital camera mounted on a Leica M205-C stereoscope. The contrast, colour, and brightness were adjusted using Adobe Photoshop CC. Line drawings were created in CorelDRAWX8. Specimens are housed in the collections of the Yunnan Key Laboratory for Palaeobiology, Yunnan University, Kunming, China (YKLP).
Eos 5d digital slr camera
Manufactured by Canon
The EOS 5D is a digital single-lens reflex (DSLR) camera produced by Canon. It features a full-frame CMOS sensor, supporting high-resolution image capture. The camera is designed to provide advanced photographic capabilities for diverse applications.
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5 protocols using eos 5d digital slr camera
1
Specimen Imaging and Documentation
2
Characterization of Fracture Mechanics via SLR Imaging
3
Preparation and Color Analysis of Potato Chips
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Potato tubers of different clones were washed clean and sliced from apical to basal ends. Thin slices (1 mm) were cut using a hand-held slicer. The slices were then rinsed in water to remove excess starch and blot-dried on paper towels. A deep-fryer machine containing vegetable oil was used to prepare the chips. At a constant temperature of 180 °C, the chips were fried for 2 min and then placed on a paper towel to drain off excess oil. The chips were then photographed after cooling, using a high-resolution Canon EOS 5D Digital SLR Camera (Chuncheon, Korea), and their color measured on the basis of the Snack Food Association (SFA) chip color measurement standard.
4
Quantifying Virus Plaque Formation in BHK-21 Cells
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BHK-21 cells seeded in a confluent monolayer were treated with the specified hamster-specific siRNA at 100 nM final concentration complexed with Dharmafect 4 (GE Healthcare) for 48 hours. siRNA sequences used are listed in Supplementary Table 3 . Following siRNA knockdown, each well was infected with 30 pfu of virus, and then incubated as for the plaque assay. Resultant plaques were photographed using an EOS 5D digital SLR camera (Canon) at a fixed distance. Plaque sizes were measured in pixels using ImageJ.
5
Cambrian Burgess Shale Fossil Specimens
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All specimens come from the Cambrian (Series 3, Stage 5) Burgess Shale Formation, Walcott Quarry Shale Member on Fossil Ridge, Yoho National Park, Canada. Two (USNM 83938 and 199960) specimens17 were collected in the original Walcott Quarry (“Phyllopod Bed”) and one (ROM 61116) specimen from level −210 (approximately 210 centimeters below the base of the original Walcott Quarry floor18 ). Specimens were photographed under normal and cross-polarized light and wet and dry conditions using an Olympus DSX 100 opto-digital microscope (USNM 83938 and 199960) and a Canon EOS5D digital SLR camera (ROM 61116). The studied specimens are housed at the Royal Ontario Museum (acronym: ROM) in Toronto, Canada (ROM 61116) and the National Museum of Natural History, Smithsonian Institution (acronym: USNM) in Washington D.C., USA (USNM 83938 and 199960).
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