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Il 10 mm00439614 m1

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The Il-10 Mm00439614_m1 is a laboratory equipment product. It is designed for the analysis and quantification of the Il-10 gene expression in mouse samples. The product provides a specific and sensitive method for measuring the target transcript.

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Lab products found in correlation

Il 1β mm00434228 m1, by Thermo Fisher Scientific (2 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions) Mcp1 mm00441242 m1, by Thermo Fisher Scientific (1 mentions) Tnfα mm00443258 m1, by Thermo Fisher Scientific (1 mentions) Rnaeasy lipid tissue mini kit, by Qiagen (1 mentions) Nanodrop, by Thermo Fisher Scientific (1 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Tri reagent, by Promega (1 mentions) Tri reagent, by Merck Group (1 mentions) Antisense primers, by Thermo Fisher Scientific (1 mentions) Taqman probe, by Thermo Fisher Scientific (1 mentions) Sense primer, by Thermo Fisher Scientific (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) High capacity cdna archive kit, by Thermo Fisher Scientific (1 mentions) Abi 7500 system, by Thermo Fisher Scientific (1 mentions) Perfectpure rna kit, by Thermo Fisher Scientific (1 mentions) Eagle taq master mix, by Merck Group (1 mentions) Gfap mm01253033 m1, by Thermo Fisher Scientific (1 mentions) Dnaase, by Merck Group (1 mentions) Tripure isolation reagent, by Roche (1 mentions)

Spelling variants of this product

IL-10 (509 citations) Mm00439614_m1 (5 citations)

4 protocols using il 10 mm00439614 m1

1

Quantifying Inflammatory Markers in Murine Tissues

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Total RNA was extracted with the RNAeasy Lipid Tissue Mini Kit (Qiagen, Hilden, Germany). RNA concentration was determined spectrophotometrically using NanoDrop® (Thermofisher). RNA (0.25–1 µg) was reverse transcribed with PrimeScript RT reagent kit using random hexamer primers (Takara, Kusatsu, Japan). TaqMan system was used for real-time PCR amplification. Relative gene expression was obtained after normalization to 18s RNA, using the formula 2−ΔΔcp. The following primers/probes were used: Tnfα Mm00443258_m1, Il-6 Mm00446190_m1, Il-1β Mm0043422/8_m1, Mcp1 Mm00441242_m1, Il-10 Mm00439614_m1, F4/80 Mm00802529_m1, Ask1 Mm0043883_m1, 18 s 4352930, Ucp1 Mm01244861_m1, Pgc1α Mm01208835_m1, Cidea Mm00432554_m1, Prdm16 Mm00712556_m1 (Applied Biosystems, Rotkreuz, Switzerland).
Lucchini F.C., Wueest S., Challa T.D., Item F., Modica S., Borsigova M., Haim Y., Wolfrum C., Rudich A, & Konrad D. (2020). ASK1 inhibits browning of white adipose tissue in obesity. Nature Communications, 11, 1642.
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2

Quantifying Cytokine Expression in Splenocytes

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Splenocytes (5 × 106 cells/ml, 5 ml/well) were seeded in a 6-well flat bottom plate and treated with CBD (10 μM) or VH (0.1% ethanol) for 30 min and treated with Us/o stimulation for 1-5 days. Cells were collected and resuspended in 1 ml TRI Reagent (Sigma, St. Louis, MO). RNA was isolated with RNeasy (Promega, Madison, WI) columns following TRI Reagent (St. Louis, MO) phase separation and nucleic acid precipitation. Equal amounts of RNA were reverse transcribed using a high capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA). RT-QPCR amplification was performed on an Mx3005P instrument (Stratagene) using a Taqman primer/probe for Tgfb1 (Mm01178820_m1) or Il10 (Mm00439614_m1) (Applied Biosystems) in a total reaction volume of 20 μl. Relative gene expression in terms of fold-change values were determined using the ΔΔCt method using18s rRNA as the internal reference and Day 1 VH as the control [25 (link)].
Dhital S., Stokes J.V., Park N., Seo K.S, & Kaplan B.L. (2016). Cannabidiol (CBD) Induces Functional Tregs in Response to Low-Level T Cell Activation. Cellular immunology, 312, 25-34.
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3

Gingival Cytokine Expression Analysis

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Gingival tissue was excised from around the maxillary molars for mRNA harvest. The expression of inflammation related cytokines were determined by quantitative real-time PCR (qPCR). Briefly, mRNA was extracted from gingival tissue, using the PerfectPure RNA kit (5 Prime; Fisher, Waltham, MA, USA). The RNA was reversetranscribed using the High-Capacity cDNA Archive kit (Applied Biosystems, Foster City, CA, USA) and qPCR was performed using the ABI 7500 System, according to the manufacturer’s protocol (Applied Biosystems). TaqMan probes, sense primers, and antisense primers for genes of interest or a housekeeping gene (glyceraldehyde 3-phosphate dehydrogenase, GAPDH) were purchased from Applied Biosystems. The following inventoried gene expression primers (Applied Biosystems) were used: IL-1β (Mm00434228_m1), IL-6 (Mm00446190_m1), and IL-10 (Mm00439614_m1). Gene expression has been normalized to that of GAPDH.
Duan X., Hays A., Zhou W., Sileewa N., Upadhyayula S., Wang H, & Liang S. (2018). Porphyromonas gingivalis Induces Exacerbated Periodontal Disease during Pregnancy. Microbial pathogenesis, 124, 145-151.
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4

Neocortical Expression Profile in Hydrocephalic Mice

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The neocortex of the left hemispheres from the same mice used for 1H HR-MAS NMR spectroscopy and non-injected hydrocephalic hyh mice (without any surgery, n = 7) was used to extract total RNA with Tripure Isolation Reagent (Roche, Basel, Switzerland). Contaminating DNA was removed using DNAase (Sigma-Aldrich). Retrotranscription of 3 μg of total RNA was performed with the High-Capacity cDNA Reverse transcription Kit (4374967, Applied Biosystems, Foster City, CA). For RT-PCR, 40 ng of cDNA was mixed with Eagle Taq Master Mix (Sigma-Aldrich) and TaqMan Gene Expression assay probes (IL-1α Mm01336161_m1, IL-1β Mm00434228_m1, IL-10 Mm00439614_m1, CD45 Mm01293577_m1, GFAP Mm01253033_m1, iba1 Mm00479862_gl, and Ki67 Mm01278617_m1; Applied Biosystems). Quantification was performed with an ABI Prism 7000 Sequence Detector System (Applied Biosystems). Results were expressed using the comparative double-delta Ct method (2-ΔΔCt). ΔCt values represent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) normalized expression levels and non-hydrocephalic mice as a control condition. In all cases, the slopes of the curves indicated optimal PCR conditions (slopes of 3.2–3.4).
García-Bonilla M., Ojeda-Pérez B., García-Martín M.L., Muñoz-Hernández M.C., Vitorica J., Jiménez S., Cifuentes M., Santos-Ruíz L., Shumilov K., Claros S., Gutiérrez A., Páez-González P, & Jiménez A.J. (2020). Neocortical tissue recovery in severe congenital obstructive hydrocephalus after intraventricular administration of bone marrow-derived mesenchymal stem cells. Stem Cell Research & Therapy, 11, 121.
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