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It 500

Manufactured by JEOL
Sourced in Japan

The IT-500 is a compact tabletop electron microscope designed for routine imaging and analysis. It features a high-resolution electron optical system and is capable of both secondary electron and backscattered electron imaging. The IT-500 is intended for use in a variety of laboratory settings, providing a user-friendly interface and easy-to-maintain operation.

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6 protocols using it 500

1

Synthesis and Characterization of Nano-Au/Nd2O3–Ca3Nd2O6

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Calcium nitrate, neodymium
oxalate, citric acid, sucrose, ammonia, chloroauric acid, and glucose
were purchased from Beijing Chemical Regent Co., Ltd. (Beijing, China).
Standard gases of carbon monoxide, formaldehyde, acetaldehyde, ammonia,
benzene, ethanol, sulfur dioxide, hydrogen sulfide, and carbon dioxide
in nitrogen were purchased from Beijing Yanan Gas Co., Ltd. (Beijing,
China). Distilled water was used throughout the whole experiment.
The micro-area composition and particle morphology of nano-Au/Nd2O3–Ca3Nd2O6 were investigated using a scanning electron microscope (JEOL-IT500)
and a TEM (JEOL-2100), respectively. The CL intensities were recorded
using an ultraweak luminescence analyzer manufactured at the Biophysics
Institute of Chinese Academy of Science (Beijing, China).
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2

Nano-Pd/ZnNi3Al2O7 Characterization

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Zinc acetate, nickel
chloride, aluminum nitrate, hydrochloric acid, malic acid, ammonia,
palladium chloride, and hydrazine hydrate were purchased from Beijing
Chemical Regent Co., Ltd. (Beijing, China). Various standard gases
of methyl ether, diethyl ether, formaldehyde, acetaldehyde, methanol,
ethanol, formic acid, acetic acid, ammonia, benzene, sulfur dioxide,
hydrogen sulfide, carbon monoxide, and carbon dioxide in nitrogen
were purchased from Beijing Ya-nan Gas Co., Ltd. (Beijing, China).
Distilled water was used throughout the whole experiment. The micro
area composition and particle morphology of the nano-Pd/ZnNi3Al2O7 were investigated by scanning electron
microscopy (SEM, JEOL-IT500) and TEM (JEOL-2100), respectively. The
CTL intensities were recorded using an ultraweak luminescence analyzer
manufactured at the Biophysics Institute of Chinese Academy of Science
(Beijing, China).
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3

Surface Morphology and Composition Analysis

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The surface morphology of the samples was examined using SEM (IT-500, JEOL, Japan) and atomic force microscopy (AFM; X-10, Park Systems, Suwon, Korea). The chemical composition of the bare fabric and the Lap-Cu2+-coated fabric was analyzed using SEM-EDS (7610F-Plus, JEOL, Japan).
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4

Characterizing Coatings with Multimodal Analysis

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SEM (JEOL IT500) was used to characterize the morphology of the coatings (the samples were coated with 5 nm of Pt/Pd prior to SEM measurements). XPS (PHI VersaProbe III with a monochromatic Al Kα source of 1486.6 eV) was utilized for measuring of chemical composition of the samples. UV-Vis transmittance was measured using an Agilent model 8453 UV-VIS spectrometer. Water contact angles were measured using First Ten Angstroms FTA125.
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5

Comprehensive Characterization of MOF Particles

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The surface morphologies and elemental distributions of the MOF particles were examined using a field emission-SEM instrument at an acceleration voltage of 20.0 kV (IT-500, JEOL, Japan). High-resolution transmission electron microscopy (HR-TEM) images were acquired from JEM-F200 (JEOL, Japan) transmission electron microscope with an accelerating voltage of 200 kV. Powder XRD patterns were recorded using a Rigaku SmartLab instrument. The FT-IR spectra were obtained using Jasco FT/IR-4700 (JASCO Global, Japan). The decomposition temperatures of the samples were determined based on thermogravimetric analysis (TGA1 Mettler TOLED) by heating them from 25 to 900 °C at a rate of 5 °C min−1 under air flow (50 cm3 min−1). The Brunauer–Emmett–Teller pore volume and surface area of each sample were evaluated based on the N2 adsorption/desorption isotherm obtained using an Autosorb-iQ 2ST/MP instrument (USA) at 77 K. Before the measurements, each sample was pre-heated at 100 °C under vacuum for 2 h in Autosorb-iQ 2ST/MP. The PL spectra and time-resolved PL decay curves of the powders were obtained using a Fluorolog3 instrument (HORIBA, Japan) equipped with a 254 nm excitation light source at room temperature.
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6

Characterization of Au Nanoparticles and Superamphiphobic Surfaces

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The morphology of the Au nanoparticles was imaged using a JEOL 2100 transmission electron microscope (TEM). For TEM analysis, 10 μL of 0.1 nM Au nanoparticle suspension was deposited onto the copper grid and dried. The analysis was conducted using a field emission gun instrument operating at an accelerating voltage of 200 kV. The diameter was determined using ImageJ.28 Images of the fabricated superamphiphobic substrate surface and the morphology of the supraparticles were obtained using a JEOL IT500 scanning electron microscope (SEM). Before SEM imaging, the samples were coated with a 5 nm iridium layer using a Leica ACE600 sputter coater. For the supraparticles, two elements (Au and C – representative of PS) were mapped in the SEM images using energy dispersive spectroscopy (EDS). The static contact angle of the water droplet on the superamphiphobic substrate was measured immediately after deposition using a Ramé-Hart Model 250 standard goniometer at room temperature.
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