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Superose 12 10 300 gl sec column

Manufactured by GE Healthcare
Sourced in Canada

The Superose™ 12 10/300 GL SEC column is a size exclusion chromatography column designed for the separation and purification of proteins, peptides, and other biomolecules. The column utilizes a dextran-based matrix with a fractionation range suitable for the analysis of a wide range of molecular weights. The column dimensions are 10 mm in diameter and 300 mm in length.

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2 protocols using superose 12 10 300 gl sec column

1

Multimodal Imaging and Analysis Protocol

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Radiochemistry reaction progress and purity were monitored using BIOSCAN ITLC (Eckert & Ziegler, Germany) and an Agilent 1260 infinity HPLC (Agilent Technologies, Santa Clara, CA) using Superose™ 12 10/300 GL SEC column (GE Healthcare, Chicago, IL). MRI data were acquired on a Bruker ClinScan 7 T MRI (Billerica, MA). Biodistribution samples were counted using a PerkinElmer 2470 WIZARD2 Automatic Gamma Counter (Waltham, MA). PET/CT data were acquired on an Inveon Preclinical Imaging Station (Siemens Medical Solutions, Knoxville, TN). Flow cytometry studies were performed on a BD LSRII (BD Biosciences, San Jose, CA), and data were analyzed using FlowJo software (BD Biosciences). IHC and hematoxylin and eosin (H&E) images were acquired using a Leica DFC7000T microscope (Leica Microsystems Inc).
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2

Multimodal Imaging and Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Radiochemistry reaction progress and yield were monitored using an Agilent 1260 infinity HPLC (Agilent Technologies) equipped with a Superose™ 12 10/300 GL SEC column (GE Healthcare). Immunoreactivity and biodistribution samples were counted using a PerkinElmer 2470 WIZARD2 Automatic Gamma Counter. Flow cytometry studies were performed on a BD LSRII (BD Biosciences), and data were analysed using FlowJo software (version 10.6.1, FlowJo, LLC). T2-weighted MRI scans were acquired with a multi slice RARE sequence using a 7T/30cm Bruker Biospec AVANCE 3 scanner equipped with a 12-cm gradient set, 40mm quadrature birdcage RF resonator with custom mouse bed and ParaVision 6.0.1 (Bruker BioSpin, Billerica MA). Tumour volumes were determined by manual segmentation using ITK-SNAP (version 3.8.0) and DSI Studio software. PET and T2-weighted MRI scans were co-registered using 3D Slicer (version 4.10.2). PET/CT and SPECT/CT scans were performed on Inveon Preclinical Imaging Stations (Siemens Medical Solutions) with Inveon Research Workstation (IRW) software (version 4.2, Siemens Healthcare, Germany). Complete blood count (CBC) studies were performed using the Abaxis Vetscan HM5 Hematology Analyzer (Allied Analytic).
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