Slit lamp was used to examine the graft attachment, corneal infection, and neovascularization on the day of operation and 1, 2, 4, and 8 weeks after operation. Anterior segment optical coherence tomography (AS‐OCT, RT‐100, Optovue Inc.) was done at 4 and 8 weeks after surgery. The average thickness of the central cornea was determined automatically by the built‐in OCT software. In the eighth week, swept‐source OCTA (SS‐OCTA, BM‐400K BMizar, TowardPi Medical Technology) was used for 3D view of the anterior segment and corneoscleral blood flow. The corneal epithelial cells, stromal cells, grafts, and endothelial cells were scanned using in vivo confocal microscope (Heidelberg Engineering Inc.). Carbomer Eye gel (Bausch & Lomb) was used as an immersion solution throughout the scan.
Carbomer eye gel
Manufactured by Bausch & Lomb
Sourced in United States
Carbomer Eye gel is a laboratory product that serves as a thickening and gelling agent for ophthalmic formulations. It is designed to increase the viscosity and improve the consistency of eye care products.
Automatically generated - may contain errors
4 protocols using carbomer eye gel
1
Comprehensive Ocular Assessment Protocol
2
Murine Corneal Alkali Burn Model
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Mice were anesthetized using 1% pentobarbital sodium, tropicamide phenylephrine (Santen, Osaka, Japan), and procaine hydrochloride (Alcon, Fort Worth, TX, USA) eye drops were administrated here before the operation. Filter paper discs were prepared using a 2-mm-diameter trephine and sterilized for the next step. 1 µL of a solution of sodium hydroxide at a concentration of 1 mol L−1 was dropped onto a filter paper disc. The excess liquid on the mice's ocular surface (or eyelids) was removed before the filter paper discs (with sodium hydroxide solution) were placed on the central cornea for 15 s. The ocular surface was then rinsed with 1 mL PBS 5 times. Mice were kept in a warm blanket until they woke up, and tobramycin eye drops (Alcon) and carbomer eye gel (Bausch & Lomb, Tampa, FL, USA) were applied once. The different concentrations of MOF eye drops (1, 0.5, 0.25 mg mL−1) were topically administered (5 μL each eye) on the murine corneal alkali burn model three times daily (09:00 am, 3:00 pm, and 9:00 pm). The model group received the same volume of PBS as a substitute.
3
Electroretinography in LPS-Treated Rats
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At 24 hours after the injection of LPS and after adaptation in the dark for 12 hours, the rats underwent electroretinography (ERG). The rats were anaesthetized as described above, followed by topical use of atropine sulphate, oxybuprocaine (Santen Pharmaceutical) and carbomer eye gel (Bausch & Lomb) for mydriasis, corneal anaesthesia and corneal hydration, respectively. The contact electrodes were placed on the central cornea, and two needle electrodes were placed subcutaneously near the nose and tail to act as the reference and ground electrodes, respectively. A visual electrophysiology system (Espion E3; Diagnosys UK) was used to record the maximum ERGs following a stimulus of 20 cds/m2 at a single pulse of 0.1 Hz in a completely dark background. The a wave amplitude was measured as the difference of amplitude between the baseline and the trough of the negative deflection. The b wave amplitude was measured from the trough of the a wave to the peak of the b wave. The ERGs were recorded 10 times per eye, and the mean value for each eye was calculated.
4
Electroretinography Protocol for Rat Eyes
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Following dark adaptation for 30 min, the rats were anesthetized by intraperitoneal injection with 70 mg/kg ketamine hydrochloride (Sangon Biotech). A total of 1% tropicamide eye drops were then used to dilate the rat pupils. The corneal surfaces were anesthetized using 4% hydrochloric acid Oxybuprocaine eye drops (Santen Pharmaceutical Co., Ltd., Shanghai, China). The rats were then placed onto a hot plate which was heated to ~37.5°C, in order to reduce the impact of anesthesia on the electrophysiological test. The reference electrode was subcutaneously placed on the foreheads of the rats, and the ground electrode was placed on their right ears. A silver chloride ring-recording electrode was placed on the surface of the corneas of the rats, and coated with Carbomer Eye gel (Bausch & Lomb, Rochester, NY, USA). The non-experimental eye was covered with a black cloth. A UTAS-E 2000 electrophysiological recording and analyzing system was used to examine the rat eye with the following parameters: The full-field stroboscopic white light stimulus distance was 30 cm, the pass band was 75–300 Hz, and the stimulus intensity long-flash (cd.s/cm2) had a duration of 10 µsec, with every sub-interval lasting 1 sec. The mixed maximum b-wave amplitude and the sum of the oscillatory potential (OP) amplitudes during dark adaptation were recorded. The mean values were calculated for both eyes.
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