Xestospongin c

Manufactured by Fujifilm

Sourced in Japan

Xestospongin C is a chemical compound that is extracted from certain marine sponges. It is a potent inhibitor of the inositol 1,4,5-trisphosphate receptor, which is involved in the regulation of intracellular calcium levels. Xestospongin C is commonly used as a research tool in the study of calcium signaling pathways.

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Lab products found in correlation

4 protocols using xestospongin c

Pharmacological Modulation of Angiotensin and Calcium Signaling

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Angiotensin II (Peptide Institute, Osaka, Japan), losartan (Wako Pure Chemical Industries, Osaka, Japan), PD123,319 (Sigma-Aldrich, St. Louis, MO, USA), angiotensin I (Peptide Institute, Osaka, Japan), captopril (Sigma-Aldrich, St. Louis, MO, USA), chymostatin (Sigma-Aldrich, St. Louis, MO, USA) and nickel(II) chloride hexahydrate (Sigma-Aldrich, St. Louis, MO, USA) were dissolved in water. Carvedilol (Tokyo Chemical Industry, Tokyo, Japan), xestospongin C (Wako Pure Chemical Industries, Osaka, Japan), SEA0400 (synthesized in our faculty according to the reported method [75 (link)]), 2-APB (Sigma-Aldrich, St. Louis, MO, USA) and ryanodine (Wako Pure Chemical Industries, Osaka, Japan) were dissolved in dimethyl sulfoxide (DMSO). They were added to the organ bath to obtain the desired final concentration.

Tanaka Y., Obata K., Ohmori T., Ishiwata K., Abe M., Hamaguchi S., Namekata I, & Tanaka H. (2019). Angiotensin II Induces Automatic Activity of the Isolated Guinea Pig Pulmonary Vein Myocardium through Activation of the IP3 Receptor and the Na+-Ca2+ Exchanger. International Journal of Molecular Sciences, 20(7), 1768.

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Molecular Mechanisms of Serotonin Receptor Signaling

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Sumatriptan succinate (sumatriptan), α-methylserotonin maleate (MS), 1-(3-chlorophenyl) biguanide hydrochloride (mCPBG), 5-methoxytryptamine (MT), WAY208466, LP12, 2’,3’-dideoxyadenosine
(ddAdo), 2-hydroxyestradiol (2-CE), KH7, 2,4-dithenoyl-1,2,5-oxadiazone n2-oxide (HC-056456, HC), U73122, U73343, D609, ET-18-OCH3, neomycin, spermine, and bisindolylmaleimide 1 (Bis-1) were
purchased from Merck KGaA (Darmstadt, Germany). TCB2, BW723C86, and MK212 were purchased from TOCRIS Bioscience (Bristol, UK). H-89, mibefradil (Mib), and NNC 55–0396 (NNC) were purchased
from Cayman Chemical (Ann Arbor, MI, USA). Anti 5-HT_2A receptor antibody (SR-2A (A-4); sc-166775) and anti-5-HT₄ receptor antibody (SR-4 (G-3); sc-376158) were
purchased from Santa Cruz Biotechnology, Inc. (Dallas, Texas, USA). Polyvinylidene difluoride (PVDF) membranes were purchased from Millipore (Bedford, MA, USA). The molecular weight marker
set was purchased from Bio-Rad Laboratories Inc. (Hercules, CA, USA). EzWestLumi Plus^® was purchased from ATTO Corporation (Tokyo, Japan). Xestospongin C, anti-mouse IgG antibody
conjugated peroxidase, bovine serum albumin (BSA), fraction V, and other reagent grade chemicals were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan).

SAKAMOTO C., FUJINOKI M., KITAZAWA M, & OBAYASHI S. (2021). Serotonergic signals enhanced hamster sperm hyperactivation. The Journal of Reproduction and Development, 67(4), 241-250.

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Calcium Signaling Pathway Modulation

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Xestospongin C, and thapsigargin were obtained from Wako (Japan). Triton-X100 and bovine serum albumin fraction V were from Nakalai Tesque (Japan). Stealth siRNA for IP3R1 (MSS275151), Silencer® Select pre-designed siRNA for STIM1 (S74488), and Orai1 (S99511) were from Thermo Fisher Scientific (USA). Fasiglifam and DAPI solution were obtained from AdoQ Bioscience (USA) and Dojindo (Japan), respectively.

Usui R., Yabe D., Fauzi M., Goto H., Botagarova A., Tokumoto S., Tatsuoka H., Tahara Y., Kobayashi S., Manabe T., Baba Y., Kurosaki T., Herrera P.L., Ogura M., Nagashima K, & Inagaki N. (2019). GPR40 activation initiates store-operated Ca2+ entry and potentiates insulin secretion via the IP3R1/STIM1/Orai1 pathway in pancreatic β-cells. Scientific Reports, 9, 15562.

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Investigating Cellular Signaling Pathways

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Reagents used in this study included H₂O₂ (31 wt%; Santoku Chemical Industries Co, Japan), BAPTA-AM (Merck Millipore, Billerica, MA), xestospongin C (Wako Pure Chemical Industries, Japan), SKF-96365 (Focus Biomolecules, Plymouth Meeting, PA), mefenamic acid (Tokyo Chemical Industry Co, Japan), 2-APB, AA-861 and phenylephrine (Sigma-Aldrich, St. Louis, MO).

Morihara H., Obana M., Tanaka S., Kawakatsu I., Tsuchiyama D., Mori S., Suizu H., Ishida A., Kimura R., Tsuchimochi I., Maeda M., Yoshimitsu T., Fujio Y, & Nakayama H. (2017). 2-aminoethoxydiphenyl borate provides an anti-oxidative effect and mediates cardioprotection during ischemia reperfusion in mice. PLoS ONE, 12(12), e0189948.

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