Guava easycyte 6ht 2l flow cytometry system

Intracellular ROS was determined with a 2,7-dichlorodi-hydrofluorescein diacetate (DCFH-DA) probe (Beyotime) using the Guava easyCyte 6HT-2L flow cytometry system (Merck Millipore), following treatment with objective compounds for 24 h, according to our previous study [14 (link)].

Cell cycle and apoptosis were detected using a cell cycle and apoptosis analysis kit (Beyotime, China) and Annexin V-FITC apoptosis detection kit (Nanjing KeyGen Biotech, Nanjing, China), respectively, with a Guava easyCyte 6HT-2L flow cytometry system (Merck Millipore, Boston, MA, USA), following treatment with objective compounds for 24 h, as previously described [14 (link)]. The cell cycle distribution (G0/G1, S and G2/M phases) and apoptosis proportion (early apoptosis and late apoptosis) were then calculated using NovoExpress software (ACEA Biosciences, San Diego, CA, USA). The measurement of cells in the sub-G1 phase with PI staining was also calculated to estimate the cellular apoptotic induction by tamoxifen, naringenin and quercetin.