Log In Sign up
The largest database of trusted experimental protocols

Nanos-Gal4 is a genetic tool used for targeted gene expression in Drosophila. It drives the expression of the yeast Gal4 transcription factor under the control of the nanos gene promoter, allowing for the specific activation of Gal4-responsive transgenes during early embryonic development.

Automatically generated - may contain errors

Lab products found in correlation

Tubulin gal4, by Bloomington Drosophila Stock Center (1 mentions) W1118, by Bloomington Drosophila Stock Center (1 mentions) Lidk06801, by Bloomington Drosophila Stock Center (1 mentions) Lid10424, by Bloomington Drosophila Stock Center (1 mentions) Eyeless gal4, by Bloomington Drosophila Stock Center (1 mentions) Aubhn2, by Bloomington Drosophila Stock Center (1 mentions) Aubqc42, by Bloomington Drosophila Stock Center (1 mentions) Spn e1, by Bloomington Drosophila Stock Center (1 mentions) Nanos gal4 vp16, by Bloomington Drosophila Stock Center (1 mentions) Gr1 gal4, by Bloomington Drosophila Stock Center (1 mentions) Bam gal4, by Bloomington Drosophila Stock Center (1 mentions) Daughterless gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas cas9, by Bloomington Drosophila Stock Center (1 mentions) Nsyb gal4, by Bloomington Drosophila Stock Center (1 mentions) Elav gal4, by Bloomington Drosophila Stock Center (1 mentions)

Close spelling variants of this product

Nanos-GAL4:VP16 (2 citations)

5 protocols using nanos gal4

1

Drosophila Genetic Crosses for Functional Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following lines were acquired from the Bloomington Stock Center: w1118 (BL# 38690), hsFlp,hsIscel/CyO (BL # 6934), nanos-Gal4 (BL# 32179), Tubulin-Gal4 (BL# 5138), eyeless-Gal4 (BL# 8227), In(1)whitem4 (BL# 807), Sb[V] (BL# 878), Cre (BL# 1501) lid10424 (BL# 12367), lidk06801 (BL# 10403). PSRFM1 was provided by Kristin White (Massachusetts General Hospital, Charlestown, MA), FRT40A, UTX1 (link) was provided by Andreas Bergmann (M. D. Anderson Cancer Center, Houston, TX), FRT40A, UTXΔ was provided by Jürg Müller (MPI of Biochemistry, Chromatin and Chromosome Biology, Martinsried, Germany).
Shalaby N.A., Sayed R., Zhang Q., Scoggin S., Eliazer S., Rothenfluh A, & Buszczak M. (2017). Systematic discovery of genetic modulation by Jumonji histone demethylases in Drosophila. Scientific Reports, 7, 5240.
+ Open protocol
+ Expand
2

Drosophila Genetics: Fly Stock Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols
All Drosophila stocks were maintained on cornmeal-molasses food between 23 and 25°C. qin1, Df(3R)Exel6180, Df(2L)BSC187, aubHN2, aubQC42, ago3t2, ago3t3, spn-E1, spn-EΔ125, nanos-Gal4, nanos-Gal4::VP16, and tub>GFP were obtained from the Bloomington Stock Center. Qin::GFP was a gift from P. Zamore (University of Massachusetts Medical School, Worcester, MA). UASp-GFP::Aub was a gift from P. MacDonald (University of Texas, Austin, TX). qinkumo was a gift from T. Kai (Temasek Research Laboratories, Singapore). The following trans-heterozygous allelic combinations were generated for all experimental analyses described: aubHN2/aubQC42 (aub), ago3t2/ago3t3 (ago3), spn-E1/spn-EΔ125 (spn-E), qin1/Df(3R)Exel6180 (qin), and qinkumo/Df(3R)Exel6180 (qinkumo). Trans-heterozygous mutants were used to minimize the effects of second-site mutations on the relevant chromosomes.
Andress A., Bei Y., Fonslow B.R., Giri R., Wu Y., Yates JR I.I.I, & Carthew R.W. (2016). Spindle-E cycling between nuage and cytoplasm is controlled by Qin and PIWI proteins. The Journal of Cell Biology, 213(2), 201-211.
+ Open protocol
+ Expand
3

Drosophila Germline and Follicle Cell Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
w1118 flies were used as the wild-type control, except for embryo injections, when Oregon-R flies were used. The oskar RNA null genotype was described in Jenny et al. (2006) (link) and was trans-heterozygous for oskA87 (Vanzo and Ephrussi 2002 (link)) and Df(3R)pXT103 (Lehmann and Nusslein-Volhard 1986 (link)). UAS-coupled genes were expressed in the germline using pCog-Gal4:VP16 (Rorth et al. 1998 (link)), nanos-Gal4:VP16 (Rorth et al. 1998 (link)), and mat-α4-tub-Gal4:VP16 (Bloomington stock #7062) promoters. Expression in follicle cells was under the control of GR1-Gal4 (Bloomington stock #36287), and salivary gland expression was under the control of forkhead-Gal4 (Henderson and Andrew 2000 (link)).
Jambor H., Mueller S., Bullock S.L, & Ephrussi A. (2014). A stem–loop structure directs oskar mRNA to microtubule minus ends. RNA, 20(4), 429-439.
+ Open protocol
+ Expand
4

Drosophila Mitochondrial Function Protocols

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Flies were maintained on cornmeal medium at 25°C, unless otherwise stated. w1118 was used as the wild-type control. Heteroplasmic mt:CoIT300I flies were generated previously (Hill et al., 2014 (link)) and maintained at 18°C, unless otherwise stated. Generation of the TFAM-GFP reporter lines was described previously (Zhang et al., 2016 (link)). The UASp-AOX transgenic fly was generated by subcloning the Ciona intestinalis AOX coding sequence into the pUASp vector (Rørth, 1998 (link)), followed by standard germline transformation procedures (Chen et al., 2015 (link)). UASp-mitoGFP transgenic fly was generated by cloning a fusion gene consisting of the N-terminal 40 amino acid residues of Dm citrate synthase (CG3861) and EGFP cDNA into the pUASp vector, followed by standard germline transformation procedures. Fis1 RNAi (BL#63027), cox5A RNAi (BL#58282), mtSSB RNAi lines (BL#50600), milton RNAi (BL#43173), X-linked mChFP-Rho1(BL#52280), bam-gal4 (BL#80579), and nanos-gal4 (BL#4937) were from the Bloomington Drosophila Stock Center. Drp1 RNAi (v44156) and tamas RNAi (v3135) were from Vienna Drosophila Resource Center. Vasa-GFP (#109171) came from Kyoto Drosophila Genomics and Genetics Resources.
Chen Z., Wang Z.H., Zhang G., Bleck C.K., Chung D.J., Madison G.P., Lindberg E., Combs C., Balaban R.S, & Xu H. (2020). Mitochondrial DNA segregation and replication restrict the transmission of detrimental mutation. The Journal of Cell Biology, 219(7), e201905160.
+ Open protocol
+ Expand
5

Drosophila Huntington's Disease Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fly stocks were maintained at room temperature following standard culture conditions. All fly crosses were performed at 25°C following standard genetic procedure unless otherwise specified. The following fly lines were from Bloomington Drosophila Stock Center: UAS-Htt.128Q.FL (#33808), UAS-Htt.16Q.FL (#33810), UAS-cas9 (58985), elav-Gal4 (#458), daughterless-Gal4 (#55850), nSyb-Gal4 (#51635), nanos-Gal4 (#4442).
UAS-Htt93Q-exon1 is a gift from Dr. Leslie Thompson.
Xu S., Li G., Ye X., Chen D., Chen Z., Xu Z., Daniele M., Tambone S., Ceccacci A., Tomei L., Ye L., Yu Y., Solbach A., Farmer S.M., Stimming E.F., McAllister G., Marchionini D.M, & Zhang S. (2022). HAP40 is a conserved central regulator of Huntingtin and a potential modulator of Huntington’s disease pathogenesis. PLoS Genetics, 18(7), e1010302.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!