Fluorescein isothiocyanate fitc conjugated annexin 5 propidium iodide kit

Fluorescein isothiocyanate (FITC)-conjugated Annexin V/propidium iodide (PI) Kit, anti-CD80, anti-CD86, and phycoerythrin-conjugated anti-MHC-I and anti-MHC-II were purchased from BD Biosciences (San Diego, CA, USA). Lipopolysaccharide (LPS) from Escherichia coli O111:B4 was purchased from InvivoGen (San Diego, CA, USA). Anti-phosphorylated extracellular signal-regulated kinase (ERK)-1/2 monoclonal antibody (Ab), anti-phosphorylated p38 monoclonal Ab, anti-phosphorylated c-Jun N-terminal kinase (JNK) monoclonal Ab, anti-phosphorylated inhibitor of κB (IκB)-α monoclonal Ab, anti-IκB-α monoclonal Ab, anti-nuclear factor (NF)-κB (p65) polyclonal Ab, anti-lamin B polyclonal Ab, anti-Toll-interacting protein (Tollip) monoclonal Ab, anti-suppressor of cytokine signaling (SOCS) 1 polyclonal Ab, anti-interleukin (IL)-1 receptor-associated kinase (IRAK)-M monoclonal Ab, anti-TLR4 polyclonal Ab, anti-cyclooxygenase (COX)-2 polyclonal Ab, anti- inducible nitric oxide (NO) synthase (iNOS) polyclonal Ab, and anti-β-Actin monoclonal Ab were obtained from Cell Signaling Technology (Danvers, MA, USA). Goat anti-rabbit-IgG-horseradish peroxidase (HRP) Ab and goat anti-mouse-IgG-HRP Ab were purchased from Calbiochem (Merck KGaA, Darmstadt, Germany), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was purchased from Sigma-Aldrich (St. Louis, MO, USA).