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Anti mouse igg1 alexafluor647

Manufactured by Jackson ImmunoResearch
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Anti-mouse IgG1-AlexaFluor647 is a secondary antibody conjugated with AlexaFluor647 dye, designed to detect and visualize mouse IgG1 antibodies in various immunoassays and microscopy applications.

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Lab products found in correlation

Prism 8, by GraphPad (2 mentions) Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (2 mentions) S1000exi, by Stratedigm (2 mentions) Alexa fluor 488 anti mouse igg, by Thermo Fisher Scientific (2 mentions) Saponin buffer, by BioLegend (2 mentions) Anti human cd184 cxcr4 pe, by STEMCELL (2 mentions) Anti human cd117 ckit apc, by Thermo Fisher Scientific (2 mentions) Cd117 apc, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Donkey anti-rabbit AlexaFluor488-conjugated and goat anti-mouse IgG1 AlexaFluor647-conjugated secondary antibodies (2 citations)

3 protocols using anti mouse igg1 alexafluor647

1

Quantifying Definitive Endoderm Induction

Cited 1 time
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Efficiency of definitive endoderm induction was quantified using CD184-PE (STEMCELL Technologies) and CD117-APC (Thermo Fisher Scientific) antibodies. To quantify intracellular protein content, fixed and permeabilized iHeps were stained using anti-human AAT (Santa Cruz Biotechnologies) and anti-human FOXA1 (Santa Cruz Biotechnologies) antibodies followed by anti-mouse IgG1-Alexa Fluor 647 (Jackson ImmunoResearch) and IgG2a-DyLight 488 (Jackson ImmunoResearch) antibodies (Wilson et al., 2015 (link)).
Kaserman J.E., Hurley K., Dodge M., Villacorta-Martin C., Vedaie M., Jean J.C., Liberti D.C., James M.F., Higgins M.I., Lee N.J., Washko G.R., San Jose Estepar R., Teckman J., Kotton D.N, & Wilson A.A. (2020). A Highly Phenotyped Open Access Repository of Alpha-1 Antitrypsin Deficiency Pluripotent Stem Cells. Stem Cell Reports, 15(1), 242-255.
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2

Quantifying Endoderm Induction and Hepatocyte Markers

Cited 2 times
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Endoderm induction was quantified using anti-human CD184(CXCR4)-PE (StemCell Technologies) and anti-human CD117(CKIT)-APC (ThermoFisher Scientific) conjugated monoclonal antibodies.21 (link),25 (link) To quantify intracellular protein content, iHeps were fixed in 1.6% paraformaldehyde for 20 min at 37°C and then permeabilized in saponin buffer (BioLegend). Cells were first probed using AAT (Santa Cruz Biotechnologies), AFP (Abcam), ZAAT (a kind gift from Qiushi Tang and Chris Mueller), and then anti-mouse IgG1-AlexaFluor647 (Jackson ImmunoResearch), anti-rabbit IgG-AlexaFluor488 (ThermoFisher Scientific) and anti-mouse IgG-AlexaFluor488 (ThermoFisher Scientific) antibodies. Staining quantification was performed using BD FACSCalibur or Stratedigm S1000EXi and all gating was performed using isotype-stained controls. Data analysis was performed using FlowJo (Tree Star) and Prism8 (GraphPad) software.
Kaserman J.E., Werder R.B., Wang F., Matte T., Higgins M.I., Dodge M., Lindstrom-Vautrin J., Bawa P., Hinds A., Bullitt E., Caballero I.S., Shi X., Gerszten R.E., Brunetti-Pierri N., Liesa M., Villacorta-Martin C., Hollenberg A.N., Kotton D.N, & Wilson A.A. (2022). Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity. Cell reports, 41(10), 111775.
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3

Quantifying Endoderm Induction and Hepatocyte Markers

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Endoderm induction was quantified using anti-human CD184(CXCR4)-PE (StemCell Technologies) and anti-human CD117(CKIT)-APC (ThermoFisher Scientific) conjugated monoclonal antibodies.21 (link),25 (link) To quantify intracellular protein content, iHeps were fixed in 1.6% paraformaldehyde for 20 min at 37°C and then permeabilized in saponin buffer (BioLegend). Cells were first probed using AAT (Santa Cruz Biotechnologies), AFP (Abcam), ZAAT (a kind gift from Qiushi Tang and Chris Mueller), and then anti-mouse IgG1-AlexaFluor647 (Jackson ImmunoResearch), anti-rabbit IgG-AlexaFluor488 (ThermoFisher Scientific) and anti-mouse IgG-AlexaFluor488 (ThermoFisher Scientific) antibodies. Staining quantification was performed using BD FACSCalibur or Stratedigm S1000EXi and all gating was performed using isotype-stained controls. Data analysis was performed using FlowJo (Tree Star) and Prism8 (GraphPad) software.
Kaserman J.E., Werder R.B., Wang F., Matte T., Higgins M.I., Dodge M., Lindstrom-Vautrin J., Bawa P., Hinds A., Bullitt E., Caballero I.S., Shi X., Gerszten R.E., Brunetti-Pierri N., Liesa M., Villacorta-Martin C., Hollenberg A.N., Kotton D.N, & Wilson A.A. (2022). Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity. Cell reports, 41(10), 111775.
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