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Uas mcherrycaax

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The UAS-mCherryCAAX is a genetic tool that enables the expression of a fluorescent protein, mCherry, which is targeted to the cell membrane. This tool can be used to visualize cell membranes in Drosophila studies.

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Lab products found in correlation

Uas nlsgfp, by Bloomington Drosophila Stock Center (1 mentions) Uas cd8 gfp, by Bloomington Drosophila Stock Center (1 mentions) Uas mcherry nls, by Bloomington Drosophila Stock Center (1 mentions) Htl gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas cd8 rfp, by Bloomington Drosophila Stock Center (1 mentions) Uas lifeact gfp, by Bloomington Drosophila Stock Center (1 mentions) Nsyb gal4, by Bloomington Drosophila Stock Center (1 mentions)

3 protocols using uas mcherrycaax

1

Drosophila Genetics and Imaging Protocol

Cited 3 times
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All flies were raised at 25 °C with 12 h/12 h light/dark cycle unless noted. This study: htl-LexA, pyr-Gal4/CyO, htl>FRT>stop>FRT>Gal4, UAS-Pyr:GFP, UAS-Ths:GFP and LexO-Htl:mCherry. All new transgenic injections were performed by Rainbow Transgenic Flies, Inc. Bloomington Drosophila Stock Center: UAS-CD8:GFP, UAS-CD8:RFP, UAS-mCherryCAAX, LexO-CD2:GFP, UAS-Eb1:GFP, UAS-Lifeact:GFP, UAS-nls:GFP, UAS-nls:mCherry, htl-Gal4, ths-Gal4/CyO, UAS-Dia:GFP, UAS-ΔDAD-Dia:GFP, UAS-pyrRNAi, UAS-diaRNAi, hs-Flp, {nos-Cas9}ZH-2A, and w1118. Vienna Drosophila Resource Center: htl:GFPfTRG, UAS-htlRNAi, and UAS-thsRNAi. Other sources: LexO-nsyb:GFP1–10, UAS-CD4:GFP1120 (link). LexO-mCherryCAAX15 (link). dpp-Gal4/CyO, LexO-Fz:mCherry and 1151-Gal4 from Huang et al.36 (link) (also see Supplementary Table 3).
Patel A., Wu Y., Han X., Su Y., Maugel T., Shroff H, & Roy S. (2022). Cytonemes coordinate asymmetric signaling and organization in the Drosophila muscle progenitor niche. Nature Communications, 13, 1185.
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2

Drosophila Neuronal Developmental Protocols

Cited 3 times
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worGal4, UAS-cherry::Jupiter, Sqh::GFP, Histone2A::mRFP1 (Bloomington stock center), UAS-mCherry::CAAX (Bloomington stock center), UAS-Pp1-87B-HA (Bloomington stock center), UAS-Tum::Venus41 . UAS-Ran-Q69L, UAS-Ran-T24N62 (link). UAS-Gap43::mEos30 (link). Sqh::mCherry63 (link). pUAST-attB-ALD-RockCA::VhhGFP4::HA, pUAST-attB-ALD-RockCA::mCherry (this study).
Transgenes were expressed using the neuroblast-specific driver worGal464 (link).
Zipper::mDendra2 MiMIC65 (link) line: Mi02518 was crossed to phiC31 integrase (expressed under the vasa promotor; Bloomington stock center) and the resulting progeny were injected with the mDendra2 exchange cassette65 (link). Injections were performed by BestGene. Positive lines were initially screened for loss of yellow body marker and tested for the expression of Zipper::mDendra2.
Roubinet C., Tsankova A., Pham T.T., Monnard A., Caussinus E., Affolter M, & Cabernard C. (2017). Spatio-temporally separated cortical flows and spindle geometry establish physical asymmetry in fly neural stem cells. Nature Communications, 8, 1383.
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3

Visualizing Neuronal Membrane Dynamics

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To obtain the data shown in Movies 1-5, we generated recombinations of nSyb-Gal4 as driver (Bloomington Stock Center no. 51635) and UAS-mCherryCAAX as effector (Bloomington Stock Center no. 59021) and viewed fluorescence with a confocal microscope (for more details see end of movies).
Saumweber T., Rohwedder A., Schleyer M., Eichler K., Chen Y.C., Aso Y., Cardona A., Eschbach C., Kobler O., Voigt A., Durairaja A., Mancini N., Zlatic M., Truman J.W., Thum A.S, & Gerber B. (2018). Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila. Nature Communications, 9, 1104.
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