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Jm109

Manufactured by New England Biolabs
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The JM109 is a strain of Escherichia coli bacteria that is commonly used in molecular biology laboratories. It is designed for high-efficiency transformation of plasmid DNA.

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Lab products found in correlation

Bl21 de3 plys, by Thermo Fisher Scientific (1 mentions) Neb5α competent cells, by New England Biolabs (1 mentions) Bl21 de3, by New England Biolabs (1 mentions) Hct116 cell, by American Type Culture Collection (1 mentions) Hela cell, by American Type Culture Collection (1 mentions) Hek293 cell, by American Type Culture Collection (1 mentions)

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E. coli JM109 (5 citations) Escherichia coli JM109 (2 citations)

2 protocols using jm109

1

Cholesterolicum Strain Characterization

Cited 1 time
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N. cholesterolicum NRRL 5767 was a gift from USDA ARS Culture and Patent Culture Collections, Peoria, Illinois. JM109 and NEB 5-α competent cells were from New England Biolabs. BL21(DE3)pLysS was from Invitrogen. Protein expression vectors pET-15b and pET-28a were from Novagen. pCRISPomyces-2 was a gift from Huimin Zhao [59 (link)] (Addgene plasmid # 61737; http://n2t.net/addgene:61737). Various recombinant clones obtained throughout this research were suspended in 20% glycerol for long term storage at -70°C.
Huang J.K., Samassekou K., Alhmadi H.B., VanDerway D.R., Diaz J.D., Seiver J.A., McClenahan S.W., Holt S.M, & Wen L. (2020). Knockout of secondary alcohol dehydrogenase in Nocardia cholesterolicum NRRL 5767 by CRISPR/Cas9 genome editing technology. PLoS ONE, 15(3), e0230915.
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2

Establishing Cell Lines for Protein Studies

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HeLa cells (ATCC, CCL-2), HEK293 cells (ATCC, CRL-1573) and HCT116 cells (ATCC, CCL-247) were obtained from ATCC and cultured in complete Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum as growth medium. HeLa cells stably expressing SP-S11-NHK-HA and S1-10 were established previously67 (link). UBE2G2 KO 293 T cells were established in Dr. Yihong Ye’s laboratory (NIDDK/NIH, USA). JM109 and BL21(DE3) were obtained from New England Biolabs.
Yan W., Zhong Y., Hu X., Xu T., Zhang Y., Kales S., Qu Y., Talley D.C., Baljinnyam B., LeClair C.A., Simeonov A., Polster B.M., Huang R., Ye Y., Rai G., Henderson M.J., Tao D, & Fang S. (2023). Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes. Nature Communications, 14, 4798.
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