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Anti golgin97

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Anti-Golgin97 is a monoclonal antibody that recognizes the Golgin97 protein, a member of the golgin family of structural proteins found in the Golgi apparatus. It is used as a marker for the Golgi complex in immunocytochemistry and Western blot applications.

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Lab products found in correlation

Hrp conjugated anti rabbit igg, by GE Healthcare (2 mentions) Horseradish peroxidase hrp conjugated anti mouse igg, by GE Healthcare (2 mentions) Biotinylated rca, by Vector Laboratories (2 mentions) Peroxidase labeling kit nh2, by Dojindo Laboratories (2 mentions) Anti gapdh, by Merck Group (2 mentions) Alexa546 conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Anti myc, by Merck Group (1 mentions) Alexa 488 conjugated anti mouse igg, by Thermo Fisher Scientific (1 mentions) Anti n cadherin, by Abcam (1 mentions) Anti calnexin, by Abcam (1 mentions) Ab21679, by Abcam (1 mentions) Ab167453, by Abcam (1 mentions) Ab18184, by Abcam (1 mentions) Anti mcherry, by Abcam (1 mentions) Anti snap tag, by New England Biolabs (1 mentions) Alexa fluor 568 a10037, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647 a21236, by Thermo Fisher Scientific (1 mentions) Anti myc, by Cell Signaling Technology (1 mentions) Anti pka c α, by Cell Signaling Technology (1 mentions) Forskolin f6886, by Merck Group (1 mentions)

5 protocols using anti golgin97

1

Antibodies and Lectins for Cell Analysis

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The following antibodies and lectins were used: anti-myc (mouse, clone 4A6; Millipore; catalog no.: 05-724), anti-BACE1 (mouse, clone 1A11, generous gift from Dr Bart De Strooper (40 (link))), anti-GAPDH (mouse, clone 6C5; Merck Millipore; catalog no.: MAB374), anti-Golgin-97 (rabbit, clone D8P2K, Cell Signaling Technology; catalog no.: 13192S), anticalnexin (rabbit, abcam; catalog no.: ab22595), anti-N-cadherin (rabbit, abcam; catalog no.: ab18203), horseradish peroxidase (HRP)–conjugated antimouse IgG (GE Healthcare; catalog no.: NA931V), HRP-conjugated anti-rabbit IgG (GE Healthcare, Amersham; catalog no.: NA934V), DSA (J-Chemical; catalog no.: J105), SSA letin (J-Chemical; catalog no.: J118), biotinylated RCA (Vector Laboratories; catalog no.: B-1085), Alexa546-conjugated anti-rabbit IgG (Invitrogen; catalog no.: A10040), and Alexa488-conjugated antimouse IgG (Invitrogen; catalog no.: A21202). DSA and SSA were conjugated to HRP using a Peroxidase Labeling Kit-NH2 (Dojindo), according to the manufacturer’s protocol.
Osada N., Nagae M., Nakano M., Hirata T, & Kizuka Y. (2022). Examination of differential glycoprotein preferences of N-acetylglucosaminyltransferase-IV isozymes a and b. The Journal of Biological Chemistry, 298(9), 102400.
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2

Immunofluorescence Staining of Cellular Proteins

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Following antibodies were used: anti-GAPDH (HRP-60004, Proteintech), anti-6∗His (ab18184, Abcam), anti-GFP (11814460001, Roche), anti-Myc (2278S, Cell Signaling Technology), anti-ACBD3 (H00064746-B01P, Abnova), anti-P-PKA-substrate(9621S, Cell Signaling Technology), anti-Golgin97 (13192, Cell Signaling Technology), anti-mCherry (ab167453, Abcam), anti-PKA-Cα (#4782, Cell Signaling Technology), anti-PKA-RIIα (612242, BD), anti-γ-COP (sc-393615, Santa Cruz), anti-GM130 (610822, BD Bioscience), anti-SNAP tag (P9310S, NEB), anti-Clathrin HC (ab21679, Abcam), anti-ARF1 (NB300-505, Novus), anti-β-Actin (3700S, Cell Signaling), and anti-α-Tubulin (3873S, Cell Signaling). Anti-Rabbit Alexa Fluor 488 (A21441), Alexa Fluor 568 (A10042), Alexa Fluor 647 (A21245), and anti-Mouse Alexa Fluor 488 (A21200), Alexa Fluor 568 (A10037), and Alexa Fluor 647 (A21236) for immunofluorescence staining were obtained from ThermoFisher. D/D Solubilizer (635054) was purchased from Clontech. Forskolin (F6886) was purchased from Sigma.
Jia J., Tang S., Yue X., Jing S., Zhu L., Tan C., Gao J., Du Y., Lee I, & Qian Y. (2023). An A-kinase anchoring protein (ACBD3) coordinates traffic-induced PKA activation at the Golgi. The Journal of Biological Chemistry, 299(5), 104696.
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3

Antibody and Lectin Toolkit for Cell Biology

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The following antibodies and lectins were used: anti-GnT-V (mouse, clone 706824, R&D Systems), anti-α1 sodium potassium ATPase (mouse, clone 464.6, Abcam), anti-LAMP1 (rabbit, Abcam), anti-GAPDH (mouse, clone 6C5, Merck Millipore), anti-GRP78 (BiP) (rabbit, Abcam), anti-GM130 (rabbit, clone D6B1) (Cell Signaling Technology), anti-Golgin97 (rabbit, clone D8P2K, Cell Signaling Technology), anti-Rab5 (rabbit, clone C8B1, Cell Signaling Technology), anti-Rab7 (rabbit, Cell Signaling Technology), anti-Rab11 (rabbit, Cell Signaling Technology), horseradish peroxidase (HRP)-conjugated anti-mouse IgG (GE Healthcare), HRP-conjugated anti-rabbit IgG (GE Healthcare), unconjugated-L4-PHA (J-Chemical), biotinylated-SSA (J-Chemical), biotinylated-RCA (Vector Laboratories), FITC-conjugated L4-PHA (J-Oil Mills), Rhodamine-conjugated L4-PHA (Vector Laboratories), Alexa546-conjugated anti-mouse IgG (Invitrogen), Alexa488-conjugated anti-rabbit IgG (Invitrogen). L4-PHA was conjugated to HRP using a Peroxidase Labeling Kit-NH2 (Dojindo) as described previously (22 (link)).
Osuka R.F., Hirata T., Nagae M., Nakano M., Shibata H., Okamoto R, & Kizuka Y. (2022). N-acetylglucosaminyltransferase-V requires a specific noncatalytic luminal domain for its activity toward glycoprotein substrates. The Journal of Biological Chemistry, 298(3), 101666.
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4

Immunofluorescence Labeling of Cellular Proteins

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Primary antibodies used were anti-FLAG (Sigma-Aldrich, F1804), anti-Myc and anti-HA (Medical & Biological Laboratories Co., M192-3S and M180-3, respectively), anti-LOX (Abcam, ab31238), anti-ATP7A (Abcam, ab13995), and anti–Golgin-97 (Cell Signaling Technology, 13192) antibodies. Secondary antibodies used were horseradish peroxidase–conjugated anti-mouse immunoglobulin G (IgG), anti-rabbit IgG, and anti-chicken IgY (Abcam, ab205719, ab6721, and ab6877, respectively) and Alexa Fluor 488–conjugated anti-mouse IgG and Alexa Fluor 555–conjugated anti-rabbit IgG (Thermo Fisher Scientific, A-11029 and A-31572, respectively).
Noda K., Kitagawa K., Miki T., Horiguchi M., Akama T.O., Taniguchi T., Taniguchi H., Takahashi K., Ogra Y., Mecham R.P., Terajima M., Yamauchi M, & Nakamura T. (2020). A matricellular protein fibulin-4 is essential for the activation of lysyl oxidase. Science Advances, 6(48), eabc1404.
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5

Quantitative Western Blotting Analysis

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Lysates were subjected to SDS-PAGE and transferred to nitrocellulose membranes. Total protein was quantified using REVERT stain (Li-Cor Biosciences). Primary antibodies were used according to manufacturer recommendations: anti-MUC16 [X75] (Novus Biologicals, NB6001468), anti-human cathepsin D (Cell Signaling, #2284), anti-mouse cathepsin D (Novus Biologicals, AF1029), anti-cathepsin B [D1C7Y] (Cell Signaling, #31718), anti-cathepsin L [33/2] (Santa Cruz, sc-32320), anti-LAMP1 [1D4B] (Developmental Studies Hybridoma Bank), anti-VDAC1 (Cell Signaling, #4661), anti-GOLGIN-97 (Cell Signaling, #13192), anti-CALR (Cell Signaling #12238), and anti-CATLASE (Cell Signaling, #14097). Secondary antibodies were used according to manufacturer recommendations.
Pedram K., Laqtom N.N., Shon D.J., Di Spiezio A., Riley N.M., Saftig P., Abu-Remaileh M, & Bertozzi C.R. (2022). Lysosomal cathepsin D mediates endogenous mucin glycodomain catabolism in mammals. Proceedings of the National Academy of Sciences of the United States of America, 119(39), e2117105119.
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