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Ab155970 rabbit

Manufactured by Abcam
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Ab155970 is a rabbit polyclonal antibody. Its core function is to detect and bind to target antigens in various experimental applications.

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Lab products found in correlation

Gtx632604 mouse, by GeneTex (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) A 11030, by Thermo Fisher Scientific (2 mentions) Dmi8 microscope, by Leica (2 mentions) A11034, by Thermo Fisher Scientific (2 mentions) Fluoromount g medium, by Southern Biotech (2 mentions)

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Ab155970 (15 citations)

2 protocols using ab155970 rabbit

1

Immunofluorescence Staining of Cellular Proteins

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Cells were fixed for 20 minutes with 4% paraformaldehyde-PBS, permeabilized with 0.5% Triton X-100 (#X100, Sigma-Aldrich) for 15 minutes and incubated with 5% BSA (Bovine Serum Albumin) in PBS for 1 hour at room temperature. After saturation, cells were incubated overnight at 4°C with anti-TMS1/ASC antibody (ASC) (#ab155970 rabbit, Abcam, 1/200 dilution) or anti-spike (S) antibody (#GTX632604 mouse, Genetex, 1/100 dilution) in PBS-BSA 5%. Then, cells were incubated with appropriate secondary antibody conjugated to Alexa Fluor 488 (#A11034, Invitrogen, 1/500) or Alexa Fluor 546 (#A11030, Invitrogen, 1/500) at room temperature for 1 hour. Nuclei were stained with Hoechst 33342 (#1874027, Invitrogen, 1/1000 dilution) for 30 minutes at room temperature. After three PBS washes, cells were mounted with Fluoromount G medium (Southern biotech). Cells were analyzed and imaged by Leica Dmi8 microscope using a 60X objective.
Lécuyer D., Nardacci R., Tannous D., Gutierrez-Mateyron E., Deva Nathan A., Subra F., Di Primio C., Quaranta P., Petit V., Richetta C., Mostefa-Kara A., Del Nonno F., Falasca L., Marlin R., Maisonnasse P., Delahousse J., Pascaud J., Deprez E., Naigeon M., Chaput N., Paci A., Saada V., Ghez D., Mariette X., Costa M., Pistello M., Allouch A., Delelis O., Piacentini M., Le Grand R, & Perfettini J.L. (2023). The purinergic receptor P2X7 and the NLRP3 inflammasome are druggable host factors required for SARS-CoV-2 infection. Frontiers in Immunology, 14, 1270081.
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2

Immunofluorescence Staining of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed for 20 minutes with 4% paraformaldehyde-PBS, permeabilized with 0.5% Triton X-100 (#X100, Sigma-Aldrich) for 15 minutes and incubated with 5% BSA (Bovine Serum Albumin) in PBS for 1 hour at room temperature. After saturation, cells were incubated overnight at 4°C with anti-TMS1/ASC antibody (ASC) (#ab155970 rabbit, Abcam, 1/200 dilution) or anti-spike (S) antibody (#GTX632604 mouse, Genetex, 1/100 dilution) in PBS-BSA 5%. Then, cells were incubated with appropriate secondary antibody conjugated to Alexa Fluor 488 (#A11034, Invitrogen, 1/500) or Alexa Fluor 546 (#A11030, Invitrogen, 1/500) at room temperature for 1 hour. Nuclei were stained with Hoechst 33342 (#1874027, Invitrogen, 1/1000 dilution) for 30 minutes at room temperature. After three PBS washes, cells were mounted with Fluoromount G medium (Southern biotech). Cells were analyzed and imaged by Leica Dmi8 microscope using a 60X objective.
Lécuyer D., Nardacci R., Tannous D., Gutierrez-Mateyron E., Deva Nathan A., Subra F., Di Primio C., Quaranta P., Petit V., Richetta C., Mostefa-Kara A., Del Nonno F., Falasca L., Marlin R., Maisonnasse P., Delahousse J., Pascaud J., Deprez E., Naigeon M., Chaput N., Paci A., Saada V., Ghez D., Mariette X., Costa M., Pistello M., Allouch A., Delelis O., Piacentini M., Le Grand R, & Perfettini J.L. (2023). The purinergic receptor P2X7 and the NLRP3 inflammasome are druggable host factors required for SARS-CoV-2 infection. Frontiers in Immunology, 14, 1270081.
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