Participants were instructed to come to the lab in a 3-hour fasted state before the initial visit. During the initial visit height (Seca Standometer; Hamburg, Germany), weight (Befour Digital Scale; Soukvill Wisconsin), percent body fat (InBody Body Composition Analyzer; Seoul, Korea), and peak aerobic capacity ( ) were measured. Subjects rested for 15 minutes in the supine position prior to analyzing body composition via bioelectrical impedance analysis to allow for fluid shifts. was conducted on a Lode Excalibur Sport cycle ergometer (Lode Medical Technology, Groningen, the Netherlands) using a graded exercise protocol until volitional fatigue. Stage one of the graded protocol was set to 95 W of resistance for 3 minutes and then increased by 35 W every 3 minutes until the subject could no longer continue. The highest oxygen consumption recorded via the metabolic measurement system (Parvo Medics TrueOne 2400 System; Sandy, UT) was defined as the . Maximum workload was calculated as the highest completed stage (in watts) + the proportion of time in the last stage multiplied by the 35 W per stage increment. The workload for the experimental trial was set at 65% of the maximum workload.
Trueone 2400 system
Manufactured by Parvo Medics
The TrueOne 2400 System is a laboratory equipment designed for the analysis of various samples. It provides accurate and reliable measurements to support scientific research and testing applications.
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Lab products found in correlation
3 protocols using trueone 2400 system
1
Aerobic Capacity Assessment Protocol
2
Kinematic and Metabolic Analysis of Gait
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Kinematic data for active, infrared markers placed bilaterally on the lateral malleoli and greater trochanters were collected using an 11 camera Qualisys Oqus camera system (QTM, Sweden). Markers on the lateral malleoli were used to measure step lengths30 (link), 33 , 64 (Fig. 1c ). Foot strike and lift-off were estimated from peak anterior and posterior lateral malleoli excursions, respectively67 (link). Stride times were defined as the time between successive foot strikes of the same limb. Ground reaction forces were recorded from force plates located under each belt.
Metabolic cost was assessed using expired gas analysis. Expired gas was sampled on a breath-by-breath basis from a mask that covered the participant’s nose and mouth, and the rate of oxygen consumption and carbon dioxide production were measured using a TrueOne® 2400 system (Parvomedics, UT).
Metabolic cost was assessed using expired gas analysis. Expired gas was sampled on a breath-by-breath basis from a mask that covered the participant’s nose and mouth, and the rate of oxygen consumption and carbon dioxide production were measured using a TrueOne® 2400 system (Parvomedics, UT).
3
Kinematic and Metabolic Assessment of Walking
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Kinematic Data. Kinematic data for passive markers placed bilaterally on the lateral malleoli and greater trochanters were collected using a 10-camera Qualisys Oqus system (QTM, Sweden). Foot strike and lift-off were estimated from peak anterior and posterior lateral malleoli excursions, respectively. 33 Metabolic Cost. Metabolic cost was assessed using expired gas analysis. Inspired oxygen (O 2 ) and expired carbon dioxide (CO 2 ) volumes were sampled on a breath-by-breath basis using a TrueOne 2400 system (Parvomedics, UT). Substrate use during the experiment was determined using the respiratory exchange ratio (RER), which is the ratio of CO 2 produced to O 2 consumed (VCO 2 /VO 2 ). VO 2 and VCO 2 were used to calculate metabolic power using a standard equation. 34 The average metabolic power from a standing baseline trial was subtracted from measurements made during all subsequent walking periods to yield net metabolic power. Net metabolic power for each treadmill trial was averaged over the last 2 minutes of walking. Metabolic data were supplemented with self-reported ratings of perceived Treadmill speed was significantly lower than overground speed (P < .001), in agreement with previous studies. 49, (link)56, 57 exertion (RPEs), 35 which were collected after each trial in 18 of 24 participants.
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