Rabbit polyclonal caspase 1 p10 m 20 antibody

LPS (E coli 0111:B4) and adenosine triphosphate (ATP) were obtained from Sigma-Aldrich (St. Louis, MO). Rabbit polyclonal caspase-1 P10 (M-20) antibody was sourced from Santa Cruz, CA. Rabbit polyclonal TLR4, IRF-1, IL-1β, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody were all from Cell Signaling Technology (Boston, MA). Rabbit polyclonal Histone H3 antibody and Rabbit polyclonal ASC antibody was obtained from ImmunoWay Biotechnology Co (Newark, DE). Alexa555-conjugated secondary antibody was obtained from Molecular Probes Inc (Eugene, OR).

LPS (Escherichia coli 0111:B4) and the specific caspase-1 inhibitorAc-YVAD-CMK were obtained from Sigma-Aldrich (St. Louis, MO, USA). The specific caspase-3 inhibitor Z-Asp-Glu-Val-Asp-fluoromethylketone (Z-DEVD-FMK) was purchased from BioVision (Milpitas, CA, USA). Rabbit polyclonal caspase-1 P10 (M-20) antibody was from Santa Cruz (Santa Cruz, CA, USA). Rabbit cleaved caspase-3 and GAPDH antibody were all obtained from Cell Signaling (Boston, MA, USA). Rabbit NLRP3 antibody was obtained from NOVUS (Littleton, CO, USA). Rabbit polyclonal ASC antibody was obtained from Immunoway (Newark, NJ, USA). FLICA® 660 Caspase-1 Assay Kit, far-red fluorescence was obtained from ImmunoChemistry (Blooming, MN, USA), and PI from Life Technologies (Carlsbad, CA, USA).