Ros assay kit s0033

Bulk black phosphorus crystal (purity > 99.9%) was purchased from Nanjing Muke Nanotechnology (Nanjing, China). Iron (II) chloride and aqueous ammonia were purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). Doxorubicin hydrochloride, methylene blue and N-methylpyrrolidone (NMP) were purchased from Aladdin (Shanghai, China). DMEM-H, RPMI-1640, fetal bovine serum (FBS), phosphate buffer saline of pH 7.2–7.4 (PBS), trypsin-EDTA, the penicillin-streptomycin solution were purchased from Gibco, Thermo Fisher Scientific. Cell counting kit-8 (CCK-8) was purchased from APExBio Technology (Shanghai, China). H₂O₂ assay kit (S0038) and ROS assay kit (S0033) were purchased from Beyotime Biotechnology (Shanghai, China). Fe₃O₄@PEI nanoparticle was obtained from Xi’an Ruixi Biological Technology (Xi’an, China). Annexin V-FITC/PI apoptosis detection kit and Calcein-AM live-cell staining kit were purchased from Solarbio Life Sciences (Beijing, China).

Zearalenone (ZEA), and 4-phenylbutyrate (4-PBA), were purchased from Sigma–Aldrich (St. Louis, MO, USA); Fetal bovine serum (FBS) and DMEM/F-12 medium were obtained from Gibco (Grand Island, NY, USA); the cell counting kit-8 (CCK8) was purchased from Dojindo Laboratories (Kumamoto, Japan); the LDH release assay, ROS assay kit (S0033) and ATP assay kit were obtained from Beyotime Institute of Biotechnology (Shanghai, China); and the cell cycle assay caspase 3 activity assay kit and Annexin V/propidium iodide (PI) were purchased from Becton Dickinson Company (BD; Franklin Lakes, NJ, USA). Dorsomorphin dihydrochloride (HY-13418) was purchased from Medchem express (Shanghai, China).

TA (CAS No.: 480-18-2, purity 99.97%, HY-N0136) and DON (CAS No.: 51481-10-8, purity 99.397%, HY-N6684) were purchased from MCE (Monmouth Junction, NJ, USA). DMEM medium was procured from Gibco (Waltham, MA, USA), and fetal bovine serum (FBS) was procured from XP BioMed (VivaCell, Shanghai, China). CCK-8 reagent was obtained from APExBIO (K1018, Houston, TX, USA). The RNA Quick Purification kit (RN001) we used was purchased from ES science (Shanghai, China). RT-qPCR SYBR Green Master Mix (11202ES08) was purchased from Yeasen Biotechnology (Shanghai, China). Antibody Ki67 (NB500-170) was procured from Novus (Littleton, CO, USA). Occludin (#91131) was procured from CST (Danvers, MA, USA). Nrf2 (380773) was procured from Zenbio (Chengdu, China). NQO1 (11451-1-AP) was procured from Proteintech (Wuhan, China). Goat antirabbit IgG (abs20002) was procured from Absin (Shanghai, China). Cy3-conjugated goat antirabbit IgG (PMK-014-096M) was procured from Bioprimacy (Wuhan, China). ECL reagent (P10200) was obtained from NCM Biotechnology (Suzhou, China). The ROS Assay Kit (S0033S) and TUNEL kit (C1086) were purchased from Beyotime (Shanghai, China). BCA protein kits (23225) were obtained from Thermo Fisher (Waltham, MA, USA).