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Model 402

Manufactured by Gilson
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Sourced in France

The Gilson Model 402 is a programmable syringe pump designed for precise and reliable liquid handling. It features a stepper motor-driven syringe mechanism that offers accurate volume control and flow rate capabilities.

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Lab products found in correlation

Lc 10advp, by Shimadzu (2 mentions) Hypersil bds c18 column, by Thermo Fisher Scientific (2 mentions)

2 protocols using model 402

1

HPLC Analysis of Microdialysis Samples

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Microdialysis aliquots (20 μL) were injected onto the analytical column (BDS Hypersil C18 column, 150 × 2.1 mm, 3 μm particle size; Thermo Scientific, Waltham, MA, USA) by a refrigerated microsampler system, consisting of a syringe pump (model 402; Gilson, France) and a multi-column injector (model 233 XL; Gilson). The column was held at a constant temperature of 32.5 °C by a column oven (Intro; Antec Leyden, the Netherlands). Chromatographic separation was isocratic at a flow rate of 0.35 mL/min by an HPLC pump (model LC-10AD vp; Shimadzu, Tokyo, Japan). The mobile phase consisted of an NaAc buffer (4.1 g/L) with MeOH (2.5% v/v), EDTA (150 mg/L), OSA (150 mg/L) and TMA (150 mg/L) and adjusted with glacial acetic acid to pH 4.1 (Cremers et al., 2007 (link)).
Ihle E.C., van der Hart M., Jongsma M., Tecott L.H, & Doupe A.J. (2015). Dopamine physiology in the basal ganglia of male zebra finches during social stimulation. The European Journal of Neuroscience, 41(12), 1506-1514.
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2

Microdialysis Sample Preparation and HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Microdialysis aliquots (20 µl) were injected onto the analytical column (BDS Hypersil C18 column, 150 × 2.1 mm, 3 µm particle size, Thermo Scientific, USA) by a refrigerated microsampler system, consisting of a syringe pump (Gilson, model 402, France) and a multi-column injector (Gilson, model 233 XL, France). The column was held at a constant temperature of 32.5° Celsius by a column oven (Intro, Antec Leyden, the Netherlands). Chromatographic separation was isocratic at a flow rate of 0.35 ml/min by an HPLC pump (Shimadzu, model LC-10AD vp, Japan). The mobile phase consisted of a NaAc buffer (4.1 g/l) with MeOH (2.5 % v/v), EDTA (150 mg/l), OSA (150 mg/l), and TMA (150 mg/l) and adjusted with glacial acetic acid to pH = 4.1 (Cremers et al., 2007 (link)).
Ihle E.C., van der Hart M., Jongsma M., Tecott L.H, & Doupe A.J. (2015). Dopamine physiology in the basal ganglia of male zebra finches during social stimulation. The European Journal of Neuroscience, 41(12), 1506-1514.
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