Spark 10 m multimode plate reader

Manufactured by Tecan

Sourced in Switzerland

The Spark 10 M multimode plate reader is a versatile laboratory instrument designed for a wide range of applications in life science research and drug discovery. It performs absorbance, fluorescence, and luminescence measurements on microplates. The Spark 10 M is capable of reading 6- to 384-well plates and offers a flexible and customizable configuration to meet the needs of various experimental protocols.

Automatically generated - may contain errors

Lab products found in correlation

Multidrop combi reagent dispenser, by Thermo Fisher Scientific (1 mentions) D300e digital dispenser, by Tecan (1 mentions) Genesys 30 spectrophotometer, by Thermo Fisher Scientific (1 mentions) Polystyrene tube, by BD (1 mentions) Adp glo kinase assay kit, by Promega (1 mentions) Ab65300, by Abcam (1 mentions)

7 protocols using spark 10 m multimode plate reader

Automated Screening of Drug Sensitivity in GBM1 and 407p Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell lines GBM1 and 407p with wild-type and inhibited CBF1 were tested for their sensitivity towards a collection of clinically approved drugs by automated screening. Inhibitors were tested in 384-well plates in 9 dilution steps with a concentration range from 0.005 to 25 μM using D300e Digital Dispenser (Tecan, Zurich, Switzerland). The cell suspension was dispensed in neurosphere media at the appropriate cell concentration per well with a volume of 30 μl per well using Multidrop Combi Reagent Dispenser (Thermo Fisher Scientific, Waltham, MA, USA) followed by incubation for 72 h. We predefined the optimal cell number of 3000 cells per well for all tested cell lines by using pLKO.1 control cells. Viability readout was carried out by adding 30 μl of the CellTiter Glo Reagent (1:1 diluted with PBS) into the wells, shaking the plate at about 900 r.p.m. for 2 min and incubating the plates for 10 min in the dark followed by luminescence quantification using Spark 10M multimode plate reader (Tecan).
Targeted drug assays with selected drugs were performed with Titer Blue assay similarly as described above.

Maciaczyk D., Picard D., Zhao L., Koch K., Herrera-Rios D., Li G., Marquardt V., Pauck D., Hoerbelt T., Zhang W., Ouwens D.M., Remke M., Jiang T., Steiger H.J., Maciaczyk J, & Kahlert U.D. (2017). CBF1 is clinically prognostic and serves as a target to block cellular invasion and chemoresistance of EMT-like glioblastoma cells. British Journal of Cancer, 117(1), 102-112.

+ Open protocol

+ Expand

Quantifying Spinal Cord MMP Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols

At each time point, mice were deeply anaesthetised with isoflurane, and a 5 mm length of spinal cord centred at the injury epicentre was immediately dissected for MMP activity assay. Dissected spinal cords were homogenised in Tris-HCl buffer containing 0.1% Triton-X-100, and assessed using mouse MMP-2 and MMP-9 activity assays (QuickZyme Biosciences, Leiden, the Netherlands). Samples and standards were added to a microplate that was pre-coated with MMP-2 or MMP-9 capture antibody. The detection enzyme and substrate were added to each well. Absorbance at 405 nm was read at 0, 6, and 22 h using a Spark 10 M multimode plate reader (TECAN, Männedorf, Switzerland). A standard curve was used to determine the concentration of MMP-2 or MMP-9 in each sample (ng/mL).

Shiraishi Y., Kimura A., Matsuo O., Sakata Y., Takeshita K, & Ohmori T. (2019). Short-term inhibition of fibrinolytic system restores locomotor function after spinal cord injury in mice. Scientific Reports, 9, 16024.

+ Open protocol

+ Expand

Bacterial Growth Kinetics in BHI

Check if the same lab product or an alternative is used in the 5 most similar protocols

Overnight cultures of the indicated strains were grown in BHI broth to an optical density at 600 nm (OD₆₀₀) of 0.1 to 0.3. Cultures were diluted in BHI broth or Pneumacult culture media to an OD₆₀₀ of 0.01 in 17-mm-diameter polystyrene tubes (Falcon, 352057) or 96-well plates. Strains in tubes were grown at 37 °C in 5% CO₂ for 7 h, and the OD₆₀₀ readings were measured every 1 h using the GeneSys 30 spectrophotometer (Thermo). Strains in 96-well plates were grown at 37 °C for 7 h inside a Tecan Spark 10 M multimode plate reader, and OD₆₀₀ readings were taken every 10 min after 30 s of shaking. Doubling times of strains grown in BHI were quantified using a python script, which is available at https://github.com/jadechun.

Chun Y.Y., Tan K.S., Yu L., Pang M., Wong M.H., Nakamoto R., Chua W.Z., Huee-Ping Wong A., Lew Z.Z., Ong H.H., Chow V.T., Tran T., Yun Wang D, & Sham L.T. (2023). Influence of glycan structure on the colonization of Streptococcus pneumoniae on human respiratory epithelial cells. Proceedings of the National Academy of Sciences of the United States of America, 120(13), e2213584120.

+ Open protocol

+ Expand

Quantifying Bacterial Biofilm Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The formation of biofilm was measured as described in ref. 84 (link) with slight modifications. Cultures were grown in BHI broth at 37 °C in 5% CO₂ in 17-mm-diameter polystyrene tubes (Falcon, 352057) to an OD₆₂₀ of 0.5 to 0.7. The culture was discarded, and the biofilm formed on the side of the tube was washed twice gently with 500 µL PBS. Next, 500 µL 0.15% (w/v) crystal violet dissolved in 8.2% (v/v) ethanol and 0.4% (v/v) methanol was added and incubated for 20 min at room temperature. The staining solution was decanted, and the biofilm was rinsed four times with 1 mL PBS. Retained crystal violet was solubilized with 2 mL 95% (v/v) ethanol for 20 min. Samples were diluted twofold in 95% (v/v) ethanol. The A₅₅₀ of the dissolved crystal violet was measured in 96-well plates using a Tecan Spark 10 M multimode plate reader and normalized to the OD₆₂₀ of the culture.

+ Open protocol

+ Expand

Measuring Diacylglycerol Kinase Alpha Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Purified GST-DGKα activity was measured in the presence and absence of an excess of commercial GST-WASp protein (Abnova). We used the DGKA Kinase Enzyme System (Promega) and ADP-Glo Kinase Assay kit (Promega) following the manufacturer’s instructions. The assay was performed in a total volume of 5 μl (white 384-well microplate) comprising 100μM dilauroylglycerol, 50 µM DTT, 100 µM ATP and, where indicated, 2mM CaCl₂. After 90 minutes of incubation, the ADP Glo reagent was added (5 µl/well; 40 minutes) followed by Kinase Detection Reagent (10 µl/well; 40 minutes). The luminescence was measured using Tecan Spark 10 M Multimode Plate Reader. E. coli DGKA (Merk) was used as a positive control, while mock purifications from GFP-transfected cells and no enzyme samples were used as background signals. The data were analysed as:
DGKα activity = (sample luminescence – no enzyme)/(GST-DGKα activity – no enzyme) *100

Velnati S., Centonze S., Rossino G., Purghè B., Antona A., Racca L., Mula S., Ruffo E., Malacarne V., Malerba M., Manfredi M., Graziani A, & Baldanzi G. (2023). Wiskott-Aldrich syndrome protein interacts and inhibits diacylglycerol kinase alpha promoting IL-2 induction. Frontiers in Immunology, 14, 1043603.

+ Open protocol

+ Expand

Quantifying HepG2 Cellular Uptake of COEs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Prior the experiments, HepG2 cells were cultured in DMEM supplemented with 10% FBS. Cells were then harvested from culture and washed three times with HBSS. Then cell density was adjusted to be around 4 × 10⁶ mL⁻¹. In a 96-well PCR plate, 100 μL of the cell suspension was mixed with 100 μL of a COE solution in HBSS in each well. The final concentration of COEs was 20 μM. The plate was sealed prior incubation. After the incubation at 37 °C for 2 hours, the cells were centrifuged at 400 × g for 10 minutes and 100 μL of supernatant from each well was transferred to a flat bottom 96-well plate. Amount of the COEs left in the supernatant was determined by measuring absorbance of the supernatant at 360 nm using Tecan Spark 10M Multimode Plate Reader.

Limwongyut J., Nie C., Moreland A.S, & Bazan G.C. (2020). Molecular design of antimicrobial conjugated oligoelectrolytes with enhanced selectivity toward bacterial cells. Chemical Science, 11(31), 8138-8144.

+ Open protocol

+ Expand

Measuring Cathepsin B Activity in BMI1-Modulated Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

WB^Ctrl/BMI1, PLC^Ctrl/BMI1 or MHCC97H-shBMI1-1/−2/MHCC97H-scr cells were used to perform CTSB activity measurement following the manufacturer’s instruction (Abcam-#ab65300). In brief, 6 × 10⁵ cells/well were added into 6-well plates and grown for 48 h. Later, the cells were lysed with CB Cell Lysis Buffer, and the supernatants were collected and centrifuged at 12,000 g for 20 min at 4 °C. Afterwards, the protein concentration of each sample was measured by using a BCA Protein Assay Kit. 50 μL of the cell lysate containing the same amount of protein and 50 μL CB reaction buffer was added to each well, respectively. Finally, 2 μL CB substrate was added to each reaction and incubated at 37 °C for 1.5 h. The fluorescence signal (Ex/Em: 400 /505 nm) was read and measured by using a Tecan Spark® 10 M Multimode plate reader. For the BMI1 inhibitor experiments, the cells were treated with either DMSO, 1/10 μM PCT209 or 25/50 μM PRT4165 for 24 h before subjected to CTSB activity measurement.

Xu L.B., Qin Y.F., Su L., Huang C., Xu Q., Zhang R., Shi X.D., Sun R., Chen J., Song Z., Jiang X., Shang L., Xiao G., Kong X., Liu C, & Wong P.P. (2023). Cathepsin-facilitated invasion of BMI1-high hepatocellular carcinoma cells drives bile duct tumor thrombi formation. Nature Communications, 14, 7033.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!