Sodium pyruvate

Manufactured by AppliChem

Sourced in United States, Germany

Sodium pyruvate is a chemical compound that serves as a source of pyruvate, a key intermediate in cellular metabolism. It is commonly used in various laboratory applications, particularly in cell culture media and as a metabolic substrate for biochemical assays.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Dnase 1, by Merck Group (2 mentions) Rpmi 1640 medium, by Harvard Bioscience (2 mentions) Percoll, by GE Healthcare (2 mentions) Penicillin, by GE Healthcare (2 mentions) Streptomycin, by GE Healthcare (2 mentions) 100 μm cell strainer, by BD (2 mentions) Imdm medium, by PAN Biotech (2 mentions) Facscalibur, by BD (1 mentions) Hla dr, by BD (1 mentions) Hepes, by AppliChem (1 mentions) Cd105, by BD (1 mentions) Indomethacin, by Cayman Chemical (1 mentions) Oil red o, by Merck Group (1 mentions) 3 isobutyl 1 methylxanthine, by Cayman Chemical (1 mentions) Tgf β1, by R&D Systems (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Streptomycin, by AppliChem (1 mentions) Linoleic acid, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

5 protocols using sodium pyruvate

Multilineage Differentiation of MSCs

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50,000 cells were labeled with 3 µl antibody or corresponding isotype control (Isotype IgG2a (BD, #553456), CD45 (BD, #555492), HLA-DR (BD, #559866), CD14 (BD, #557153), CD73 (BD, #561254), CD105 (BD, #561443), Isotype IgG2aκ (BD #555573), Isotype IgG1 (Milteny, #130-081-002), CD11b (Milteny, #130-081-201), CD34 (Milteny, #130-092-213), CD90 (Milteny, #130-095-403), CD19 (Milteny, #130-091-328)) and analyzed using a Beckton Dickinson FACS Calibur).
To induce adipogenesis, confluent monolayers were treated with DMEM containing 1 μM dexamethasone, 0.01 mg/ml insulin (Berlinchemie), 0.2 mM indomethacin (Cayman Chemical Company), and 0.5 mM 3-isobutyl-1-methyl-xanthine (Serva) for 4 weeks. After fixation with 4% paraformaldehyde, staining was performed with 6 ml of 0.5% Oil red O (Sigma) in isopropanol added to 4 ml deionized water.
Chondroblastic differentiation was tested in pellet cultures with 1 × 10⁶ cells in 4 ml DMEM containing 1 mM sodium pyruvate (Applichem), 20 mM HEPES (Roth) pH 7.3, 0.1 µM dexamethasone, 0.1 mM 2-phospho-L-ascorbic acid, and 10 ng/ml TGF-β1 (R&D). After 4 weeks, protein extraction and western blot analysis were performed.

Hegner B., Schaub T., Janke D., Zickler D., Lange C., Girndt M., Jankowski J., Schindler R, & Dragun D. (2018). Targeting proinflammatory cytokines ameliorates calcifying phenotype conversion of vascular progenitors under uremic conditions in vitro. Scientific Reports, 8, 12087.

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Anticancer Peptides Evaluated on Cancer and Normal Cell Lines

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Anticancer activity of the peptides (Aur and designed analogs) was investigated on cancer cell lines, including: 1) SW480 (Colon carcinoma cancer cell line) and 2) HT29 (Human colorectal adenocarcinoma cell line) as well as normal cell lines, including: 3) KDR (Human Kidney Epithelial cell line) and 4) HUVEC (Human Umbilical Vein Endothelial Cell line). All cell lines were purchased from the Pasteur Institute (Tehran, Iran). The cells were grown on the 25 cm² cell culture flasks containing RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (Invitrogen, Carlsbad, CA, USA), sodium pyruvate (1 mM), penicillin G100 U/mL, and streptomycin 100 μg/mL (AppliChem, Darmstadt, Germany) in a humidified atmosphere containing 5% CO2 and 95% air at 37°C.

Salarpour Garnaie H., Shahabi A., Geranmayeh M.H., Barzegar A, & Yari Khosroushahi A. (2022). Designing Potent Anticancer Peptides by Aurein 1.2 Key Residues Mutation and Catenate Cell-Penetrating Peptide. Advanced Pharmaceutical Bulletin, 13(3), 583-591.

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Isolation of Lung Leukocytes from Tissue

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Lung tissue was cut into small pieces and digested for 30 min at 37°C in RPMI 1640 medium (Biochrom, Berlin, Germany) supplemented with DNase I (111 U/ml; Sigma-Aldrich, Taufkirchen, Germany), Collagenase D (0.7 mg/ml; Roche Diagnostics Deutschland GmbH, Mannheim, Germany), and 1 mM sodium pyruvate (AppliChem, Darmstadt, Germany). Following passage through 100 μm cell strainers (BD Biosciences, Heidelberg, Germany), erythrocytes were lysed as described above. Leukocytes were separated from tissue cells by 30%/70% Percoll (GE Healthcare, Uppsala, Sweden) gradient centrifugation (400 × g, 20 min, RT). Cells were recovered from the interphase and resuspended in IMDM medium (PAN-Biotech, Aidenbach, Germany) supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin (both purchased from PAA Laboratories), and 10% FBS (Thermo Fisher Scientific, Carlsbad, USA; and PAN-Biotech, Aidenbach, Germany).

Rabiger F.V., Rothe K., von Buttlar H., Bismarck D., Büttner M., Moore P.F., Eschke M, & Alber G. (2019). Distinct Features of Canine Non-conventional CD4−CD8α− Double-Negative TCRαβ+ vs. TCRγδ+ T Cells. Frontiers in Immunology, 10, 2748.

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Isolation of Lung Leukocytes

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Tissue pieces of lung (3 x 3 x 0.5 cm³) were minced and digested for 30 min at 37°C in RPMI1640 medium (Biochrom, Berlin, Germany) supplemented with DNase I (111 U/ml; Sigma-Aldrich, Taufkirchen, Germany), Collagenase D (0.7 mg/ml; Roche Diagnostics Deutschland GmbH, Mannheim, Germany), and 1 mM sodium pyruvate (AppliChem, Darmstadt, Germany) with slow agitation. Single cell suspensions were obtained by passaging digested lung tissue through 100 μm cell strainers (BD Biosciences, Heidelberg, Germany). Subsequently, erythrocytes were lysed as described above. For enrichment of leukocytes, the cells were resuspended in 70% Percoll (GE Healthcare, Uppsala, Sweden) diluted in RPMI1640 medium and layered under 30% Percoll. Following density gradient centrifugation (400 x g, 20 min, RT), leukocytes were recovered from the interphase, resuspended in IMDM medium (PAN-Biotech, Aidenbach, Germany) supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin (both purchased from PAA Laboratories), and 10% FBS (Thermo Fisher Scientific, Carlsbad, USA). The cell number was determined as described above.

Rabiger F.V., Bismarck D., Protschka M., Köhler G., Moore P.F., Büttner M., von Buttlar H., Alber G, & Eschke M. (2019). Canine tissue-associated CD4+CD8α+ double-positive T cells are an activated T cell subpopulation with heterogeneous functional potential. PLoS ONE, 14(3), e0213597.

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Chondrogenic Re-differentiation of Cells

Cited 1 time

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Chondrocyte phenotype was determined under re-differentiation. For chondrogenic re-differentiation, a pellet culture assay with chondrogenic differentiation media (ChM) was performed for 21 days in a 96 deep-well format as described elsewhere [43 (link),44 (link)]. Briefly, 3 × 10⁵ cells per pellet were used, and cultured in ChM composed of high glucose DMEM including L-glutamine (Sigma Aldrich, D6546, USA), dexamethasone (0.1 µM, Sigma Aldrich, D2915, USA), L-ascorbic acid (50 µg/mL, Sigma Aldrich, A8960, USA), L-proline (40 µg/mL, Sigma Aldrich, P5607, St. Louis, MO, USA) sodium pyruvate (0.1 mg/mL, AppliChem, A4859, Darmstadt, Germany) ITS (6.25 µg/mL, Sigma Aldrich, I1884, St. Louis, MO, USA), BSA (1.25 mg/mL, Sigma Aldrich, A9418, St. Louis, MO, USA), linoleic acid (5.35 µg/mL, Sigma Aldrich, L1012, St. Louis, MO, USA), penicillin (10 U/mL)/streptomycin (10 µg/mL) (Biochrom, A2213, Berlin, Germany) and recombinant human TGF-ß1 (10 ng/mL, Peprotech, 100-21-5, Cranbury, NJ, USA). Negative controls were cultured in the same medium but without TGF-ß1. Media change was performed every 3–4 days. Chondrogenic phenotype was determined by proteoglycan assay, type II collagen immunostaining, Alcian blue staining, as well as by expression of type II collagen, type X collagen, MMP13, Sox9 and aggrecan mRNAs.

Textor M., Hoburg A., Lehnigk R., Perka C., Duda G.N., Reinke S., Blankenstein A., Hochmann S., Stockinger A., Resch H., Wolf M., Strunk D, & Geissler S. (2023). Chondrocyte Isolation from Loose Bodies—An Option for Reducing Donor Site Morbidity for Autologous Chondrocyte Implantation. International Journal of Molecular Sciences, 24(2), 1484.

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