Viable count was performed by the spreading plate method for the bacterial suspensions and inoculated bacteria enumeration, while pouring plate method was used for the natural microflora enumeration of prawn. The inoculated bacteria on cubed material with or without LT-HPCD treatment were collected aseptically by vortex blending the material in sterile 0.85% NaCl solution for 15 s. Subsequently, the bacteria suspension was ten-fold serially diluted with 0.85% NaCl solution, and 0.1 ml of each dilution was spread on LB agar. After incubated at 37 °C for 12 h, the forming colonies were counted. Prawn sample (25 ± 1 g) were homogenized with 225 ml of 0.85% NaCl solution for 2 min in a stomacher bag using a stomacher (BagMixer400 VW, Interscience, France). The homogenate was used for pH analysis (FiveEasy Plus FE28, METTLER TOLEDO, Switzerland) and microbial enumeration after being serially diluted. One-milliliter of each dilution was poured in plate count agar (CM0325, OXOID, England) followed by incubation at 30 °C for 72 h, and the total bacteria were counted. For each treatment, three replications were performed.
Bagmixer 400 vw
Manufactured by Interscience
Sourced in France
The BagMixer 400 VW is a laboratory equipment designed for the homogenization of samples. It is used to mix, blend, and distribute samples within a sealed bag.
Automatically generated - may contain errors
Lab products found in correlation
Fiveeasy plus fe28, by Mettler Toledo (1 mentions)
Cm0325, by Thermo Fisher Scientific (1 mentions)
Macconkey agar plate, by Merck Group (1 mentions)
Palcam agar, by Beijing Land Bridge Technology (1 mentions)
Tryptone bile x glucuronide tbx agar, by Thermo Fisher Scientific (1 mentions)
Peptone water, by Thermo Fisher Scientific (1 mentions)
Kovac s indole reagent, by Merck Group (1 mentions)
Columbia blood agar, by Thermo Fisher Scientific (1 mentions)
Gassner agar, by Thermo Fisher Scientific (1 mentions)
8 protocols using bagmixer 400 vw
1
Viable Bacterial Enumeration in Prawn
2
Pork Patty Microbiological Assessment
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Ten grams of each pork patty sample was aseptically placed into sterile stomacher
bags (Interscience, Saint Nom la Bretêche, France) and homogenized with
90 mL sterile saline using a stomacher (BagMixer 400 VW, Interscience) for 40 s.
The homogenate was serially 10-fold diluted in sterile saline, and microorganism
populations were evaluated by the pour plate method in Petri dishes as follows:
The total aerobic bacteria (TAB) counts were measured on Plate Count Agar (PCA,
MB Cell), incubated at 37°C for 48 h; lactic acid bacteria (LAB) counts
were measured on MRS agar (MB Cell), incubated under anaerobic conditions at
37°C for 48 h, Pseudomonas spp. and Enterobacteriaceae
counts were measured on Cetrimide Agar (CN, MB Cell) and Violet
Red Bile Glucose Agar (VRBG, MB Cell), respectively, incubated at 37°C
for 24 h.
bags (Interscience, Saint Nom la Bretêche, France) and homogenized with
90 mL sterile saline using a stomacher (BagMixer 400 VW, Interscience) for 40 s.
The homogenate was serially 10-fold diluted in sterile saline, and microorganism
populations were evaluated by the pour plate method in Petri dishes as follows:
The total aerobic bacteria (TAB) counts were measured on Plate Count Agar (PCA,
MB Cell), incubated at 37°C for 48 h; lactic acid bacteria (LAB) counts
were measured on MRS agar (MB Cell), incubated under anaerobic conditions at
37°C for 48 h, Pseudomonas spp. and Enterobacteriaceae
counts were measured on Cetrimide Agar (CN, MB Cell) and Violet
Red Bile Glucose Agar (VRBG, MB Cell), respectively, incubated at 37°C
for 24 h.
3
Isolation and Detection of STEC in Lettuce
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One hundred lettuce samples were collected weekly from various farm lands in Tehran between June and September 2011. Every sample was packed in a separate stomacher bag (Interscience, France) and transported (in ice) to the laboratory, where they were immediately processed by a stomacher instrument (Bag Mixer400 VW, Interscience, France). Enrichment and processing of the samples were carried out according to previously described protocols (7 (link)). Briefly, 25 g of each sample was homogenized by the stomacher in its bag containing 225 mL E. coli broth (EC) medium (Merck, Germany), supplemented with 0.05 mg/L cefixime (Daana Pharmaceutical co., Iran). This antibiotic enhances the enrichment of STEC strains.
After overnight incubation at 37°C, a portion of the mixture was spread over a MacConkey agar plate (Merck, Germany) and was further incubated at 37°C overnight. A sweep from the semi-confluent area of the plate was used for DNA extraction for polymerase chain reaction (PCR) detection of STEC. The STEC-positive samples were subjected to further isolation processes of STEC, further PCR and antibiotic susceptibility tests.
After overnight incubation at 37°C, a portion of the mixture was spread over a MacConkey agar plate (Merck, Germany) and was further incubated at 37°C overnight. A sweep from the semi-confluent area of the plate was used for DNA extraction for polymerase chain reaction (PCR) detection of STEC. The STEC-positive samples were subjected to further isolation processes of STEC, further PCR and antibiotic susceptibility tests.
4
Aerobic Plate Count in Meatballs
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The total viable counts (TVC) for each sample were obtained using the pour plate technique according to the China National Food Safety Standard methods (Food Microbiology Examination‐Aerobic Plate Count; GB 4789.2–2016). Briefly, 25 g of meatball sample was aseptically transferred to a sterile stomacher bag and homogenized in 225 ml of sterile saline for 2 min in a stomacher (BagMixer 400 VW, Interscience Co.). After performing 1:10 serial dilutions, 1 ml of the suspension from each dilution was inoculated onto plate count agar (PCA, Beijing Luqiao) and incubated at 37°C for 48 hr to allow the total viable counts to be determined. The results were expressed as decimal logarithms of colony‐forming units per gram (LogCFU/g).
5
Presoaking synergizes ClO2 inactivation of E. coli and Salmonella on alfalfa
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To determine the synergic effect of presoaking on the inactivation of pathogenic E. coli and Salmonella spp. on alfalfa seeds using ClO 2 , ClO 2 was applied to unsoaked and presoaked seeds. Briefly, 10 g of each seed sample inoculated with pathogenic E. coli and Salmonella spp. were placed in 50 mL sterile plastic tube. Before ClO 2 treatment, seeds were immersed in 40 mL distilled water at room temperature for 5 h for soaking treatment, and then distilled water was drained. Then, 40 mL of 200 ppm NaOCl and four different concentrations of ClO 2 (50, 100, 150, and 200 ppm) were dispensed into a 50 mL plastic tube of the above samples, respectively. Each sample was treated for 15 min and the sanitizer was drained. Each treated seed was placed into a sterile plastic bag with 90 mL DE neutralizing broth (BD Difco ™ , Sparks, MD, USA) to neutralize the effect of the disinfectant. Bacteria were detached using a stomacher (Bagmixer 400VW; Interscience ® , Paris, France) for 1 min at speed 7. Homogenates (1 mL) were serially diluted in 9 mL of 0.1% peptone water, and 0.2 mL of each dilution was spread on TSA-R. Plates were incubated at 37 °C for 24 h, and the colonies were counted manually.
6
Bacterial Enumeration in Shrimp Samples
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The bacterial enumeration method used in this study was the plating counting method according to previous studies (Belletti et al., 2013a (link); Wang et al., 2014 (link)). Briefly, shrimp samples were homogenized for 2 min in a stomacher (BagMixer400VW, Interscience, France). Then, a direct-plating procedure was used for the enumeration of V. parahaemolyticus, L. monocytogenes and indigenous microflora on shrimp samples. TCBS ager for V. parahaemolyticus, PALCAM agar (Beijing Land Bridge Technology Company Ltd., Beijing, China) for L. monocytogenes and Trypticase Soy Agar (TSA; Beijing Land Bridge Technology Company Ltd., Beijing, China) for indigenous microflora was used, respectively. The detection limit of the direct-plating was approximately 2 log10 CFU/g. Colonies were counted after the plates were incubated at 37°C for 24 h. Three replicates at each sampling time were done.
7
Investigating Cloacal and Manure Samples for Bacterial Identification
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The bacteriological investigations were carried out as previously described [25 (link)]. In brief: Cloacal swab samples were directly streaked on Gassner agar plates, following an incubation overnight at 37 °C. For manure samples 50 mL of peptone water (Oxoid, Wesel, Germany) as well as the manure sample itself (25 g each) were put into a sterile Whirl-Pak® Bag (Nasco, Fort Atkinson, WI, USA). Bags were mixed for three minutes with a Bag Mixer® 400 VW (Interscience, Saint Nom, France). Using a sterile loop, 10 μL of each mixed-sample was streaked on Gassner agar (Oxoid, Wesel, Germany) and incubated at 37 °C for 18–24 h.
One single blue color colony from each plate was selected and spread onto Columbia blood agar (Oxoid, Wesel, Germany) and Tryptone Bile X-glucuronide (TBX) agar (Oxoid, Wesel, Germany). Incubation was done overnight at 37 °C. Bluegreen colonies on TBX agar detected glucuronidase activity. The positive indole test with Kovac’s indole reagent (Merck, Darmstadt, Germany) was used to confirm the diagnosis.
One single blue color colony from each plate was selected and spread onto Columbia blood agar (Oxoid, Wesel, Germany) and Tryptone Bile X-glucuronide (TBX) agar (Oxoid, Wesel, Germany). Incubation was done overnight at 37 °C. Bluegreen colonies on TBX agar detected glucuronidase activity. The positive indole test with Kovac’s indole reagent (Merck, Darmstadt, Germany) was used to confirm the diagnosis.
8
Isolation of Soil Microbes from Tomato Greenhouse
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Microbes were isolated from soil in which tomatoes grew in a greenhouse in Buyeo-gun, Chungcheong Province. Soil (3 g) was suspended in 27 ml of peptone water (PW, Difco, USA), homogenized using a stomacher (Bagmixer 400VW, Interscience, France) for 2 min at low speed, and serially diluted (10-fold). The diluted soil suspensions (100 μl) were spread on tryptic soy agar (TSA) (Difco, USA) plates and incubated at 28°C for 16 h. Single bacterial colonies were transferred to fresh TSA plates.
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