Au4500 automatic chemistry analyzer

Venous blood samples were collected using routine methods between 0700 h and 0930 h after overnight fasting. TT4, FT4, TT3, FT3, and TSH were measured with an Advia Centaur XP immunity analyzer (Siemens Corporation) and thyroid autoantibodies (TPO-Ab and TG-Ab) were measured with a Roche electrochemical immunity analyzer (E170). FPG, fasting insulin, TG, and HDL-c were measured with an Olympus AU4500 automatic chemistry analyzer (Olympus Corporation, Tokyo, Japan). The homeostasis model assessment (HOMA) index for IR (HOMA-IR) was calculated as fasting insulin (mU/L) times FPG (mmol/L) divided by 22.5.

After an overnight fast, all participants underwent a detail anthropometric evaluation including height (m), weight (kg), systolic pressure (SDP), and diastolic pressure (DBP) at 8:00 am. All of these were measured twice during the examination, and the averages of these data were used for further analysis. Body mass index (BMI) was calculated as weight/height².
Venous blood samples were drawn after a fasting period of 12h. Serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c), high- density lipoprotein cholesterol (HDL-c), fasting glucose (FG) and uric acid (UA) levels were measured using an Olympus AU4500 automatic chemistry analyzer (Olympus Corporation, Tokyo, Japan). Definition of metabolic disorders was shown in Table 3. Thyroid ultrasound examination was performed by specific thyroid experts using a 10-MHz liner array probe (Logiq 5 Pro, GE Medical systems, USA). For each nodule, size (length, width, and depth), location, number, echogenicity, boundary, and cystic component were collected and recorded. We recorded greatest nodule dimension as TN diameter. According to these results, subjects were divided into TN present group and TN absent group. The normal UA group are subjects with UA < 420μmol/L for men and < 360μmol/L for women. Otherwise, subjects were included into the high UA group.

Samples of venous blood were obtained between 6:00 and 8:00 am after overnight fasting. Triglycerides, total cholesterol, fasting plasma glucose, LDL-c, and HDL-c levels were determined with an AU4500 automatic chemistry analyzer (Olympus Corporation, Tokyo, Japan). Glycated hemoglobin (HbA1c) was measured with a TOSOH HLC-723G8 automatic glycohemoglobin analyzer (Tosoh Corporation, Yamaguchi, Japan) and fasting C-peptide with an ADVIA Centaur XP immunoassay System (Siemens Inc., Munich, Germany).