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I ab af6 1201

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The I-Ab (AF6-1201) is a lab equipment product manufactured by BD. It is designed for use in various laboratory applications. The core function of this product is to provide a tool for researchers and scientists to conduct their experiments and analyses. However, a detailed description of its specific features and intended use cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Facscalibur, by Tree Star (1 mentions) Facs canto, by Tree Star (1 mentions) Cytofix cytoperm kit, by BD (1 mentions) 7 aad, by BD (1 mentions) Cd80 16 10a1, by BioLegend (1 mentions) Ly51 6c3, by BioLegend (1 mentions) Cd45 30 f11, by Thermo Fisher Scientific (1 mentions) Ls column, by Miltenyi Biotec (1 mentions) Dnase 1, by Roche (1 mentions) Collagenase dispase, by Roche (1 mentions) Anti cd45 microbeads, by Miltenyi Biotec (1 mentions) Cd16 cd32 2.4g2, by BD (1 mentions) Foxp3 fjk 16s, by Thermo Fisher Scientific (1 mentions) Ccr7 4b12, by Thermo Fisher Scientific (1 mentions) Ccr9 242503, by R&D Systems (1 mentions) Il 17a tc11 18h10.1, by BioLegend (1 mentions)

3 protocols using i ab af6 1201

1

Multicolor Flow Cytometry Immunophenotyping

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Monoclonal antibodies (mAbs) to CD90.1 (Thy1.1, OX-7), I-Ab (AF6-1201) and IFNγ (XMG1.2) were from BD PharMingen; mAbs to CD11b (M1/70), Gr1 (RB6-8C5), MHC-II (M5/114.15.2), Vβ8.1/8.2 (KJ16), CD8α (53–6.7), PD-1 (J43), Tim-3 (RMT3-23), TNF (MP6-XT22), I-Ak (11-5.2), and I-Ek (14-4-4S) were from eBioscience. Samples were initially incubated with 2.4G2 hybridoma supernatant to block antibody binding to the Fcγ receptors. In tumor samples, dead cells were identified by 7AAD (BD Pharmingen) staining and excluded by electronic gating. Intracellular stainings were performed using the Cytofix/Cytoperm kit from BD (Cat. No. 554714). AccuCount Rainbow beads (Cat. No. ACRFP-100-3 Spherotech) were used according to the manufacturer’s instructions to determine absolute counts of 2C cells in PBL. Data were acquired on a FACSCalibur or FACSCanto and analyzed with FlowJo software (Tree Star, Ashland, OR).
Arina A., Karrison T., Galka E., Schreiber K., Weichselbaum R.R, & Schreiber H. (2017). Transfer of allogeneic CD4+ T cells rescues CD8+ T cells in anti-PD-L1–resistant tumors leading to tumor eradication. Cancer immunology research, 5(2), 127-136.
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2

Isolation and Sorting of Thymic Epithelial Cells

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Thymus tissue was enzymatically digested with Collagenase/Dispase (2.5 mg/ml; Roche) and DNase 1 (40 mg/ml; Roche). After digestion, CD45+ thymocytes were depleted using anti‐CD45 microbeads and LS columns (Miltenyi Biotec). Antibodies against the following were used to sort TEC populations: CD45 (30‐F11, eBioscience), EpCAM1 (G8.8, eBioscience), I‐Ab (AF6‐120.1, BD Bioscience), Ly51 (6C3, Biolegend), CD80 (16‐10A1, Biolegend). Reagents were conjugated to Pacific Blue, BV 421, BV605, PE, PerCP–eFluor 710, allophycocyanin–eFluor 780. Streptavidin PE‐Cy7 was used to detect biotinylated antibodies. Sorting was performed using a FACS Aria Fusion 1 sorter (BD) with a purity typically >98%.
Cowan J.E., Baik S., McCarthy N.I., Parnell S.M., White A.J., Jenkinson W.E, & Anderson G. (2018). Aire controls the recirculation of murine Foxp3+ regulatory T‐cells back to the thymus. European Journal of Immunology, 48(5), 844-854.
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3

Integrin-Mediated Immune Cell Dynamics

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The anti-integrin β1 monoclonal antibody (mAb), KMI6, was purchased from OriGene Technologies (Rockville, MD). Blocking mAbs to integrin α1 (clone HMα1), α4 (R1-2), α5 (5H10-27), β1 (HMβ1-1), and β7 (FIB27) were from BioLegend (San Diego, CA); αE/CD103 (M290) and β1 (9EG7) were from BD Biosciences (San Jose, CA). The following mAbs were used for flow cytometry and cell sorting: CD11c (N418), CD40 (1C10), CD86 (GL1), CD103 (2E7), integrin α1 (HMα1), α2 (HMα2), α4 (R1-2), α5 (5H10-27), α6 (GoH3), αV (RMV-7), β1 (HMβ1-1), β7 (FIB504), α4β7 (DATK32), IFN-γ (XMG1.2), and IL-17A (TC11-18H10.1) were from BioLegend; E-cadherin (114420) and CCR9 (242503) were from R&D Systems; CCR7 (4B12) and Foxp3 (FJK-16s) were from eBioscience (San Diego, CA); CD16/CD32 (2.4G2) and I-Ab (AF6-120.1) were from BD Biosciences.
Yokota-Nakatsuma A., Ohoka Y., Takeuchi H., Song S.Y, & Iwata M. (2016). Beta 1-integrin ligation and TLR ligation enhance GM-CSF–induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties. Scientific Reports, 6, 37914.
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