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Anti ps28 histone h3

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Anti-pS28 histone H3 is a primary antibody that specifically recognizes histone H3 phosphorylated at serine 28. Histones are core protein components of chromatin that play a crucial role in the regulation of gene expression and other cellular processes.

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Lab products found in correlation

Anti pfaldolase, by Abcam (2 mentions) Clone 3f10, by Roche (2 mentions) Complete protease inhibitor cocktail, by Roche (2 mentions) Anti tubulin, by Abcam (1 mentions) Anti pstaire, by Abcam (1 mentions) Anti histone h3, by Abcam (1 mentions)

Spelling variants of this product

Histone H3 (263 citations) Anti-Histone H3 (228 citations) Anti-H3 (148 citations)

4 protocols using anti ps28 histone h3

1

Purification and Quantification of Malaria Parasite Proteins

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Synchronized parasites were harvested and uninfected erythrocytes were removed using 0.05% saponin in PBS plus 1× cOmplete™ protease inhibitor cocktail (Roche). Upon washes, parasites were lysed in Laemmli sample buffer and proteins were resolved by SDS-PAGE, transferred to nitrocellulose, and probed with the following primary antibodies: anti-HA (1:1.000; Roche; clone 3F10), anti-Pflactate dehydrogenase (LDH) (1:2.000; gift of Michael T. Makler), anti-histone H3 (1:1.000; Abcam; ab1791), anti-pS28 histone H3 (1:1.000; Abcam; ab5169), anti-Pfaldolase (1:40.000, Abcam; ab38905). Signal intensities were quantitated using Imag J.
Ganter M., Goldberg J.M., Dvorin J.D., Paulo J.A., King J.G., Tripathi A.K., Paul A.S., Yang J., Coppens I., Jiang R.H., Elsworth B., Baker D.A., Dinglasan R.R., Gygi S.P, & Duraisingh M.T. (2017). Plasmodium falciparum CRK4 directs continuous rounds of DNA replication during schizogony. Nature microbiology, 2, 17017.
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2

Purification and Quantification of Malaria Parasite Proteins

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Synchronized parasites were harvested and uninfected erythrocytes were removed using 0.05% saponin in PBS plus 1× cOmplete™ protease inhibitor cocktail (Roche). Upon washes, parasites were lysed in Laemmli sample buffer and proteins were resolved by SDS-PAGE, transferred to nitrocellulose, and probed with the following primary antibodies: anti-HA (1:1.000; Roche; clone 3F10), anti-Pflactate dehydrogenase (LDH) (1:2.000; gift of Michael T. Makler), anti-histone H3 (1:1.000; Abcam; ab1791), anti-pS28 histone H3 (1:1.000; Abcam; ab5169), anti-Pfaldolase (1:40.000, Abcam; ab38905). Signal intensities were quantitated using Imag J.
Ganter M., Goldberg J.M., Dvorin J.D., Paulo J.A., King J.G., Tripathi A.K., Paul A.S., Yang J., Coppens I., Jiang R.H., Elsworth B., Baker D.A., Dinglasan R.R., Gygi S.P, & Duraisingh M.T. (2017). Plasmodium falciparum CRK4 directs continuous rounds of DNA replication during schizogony. Nature microbiology, 2, 17017.
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3

Affinity-purified Antibodies for Cell Cycle Analysis

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Custom rabbit polyclonal affinity–purified anti-cyclin Ba/b (acetyl-NRDLNIQESGPVKAVVNAC-amide and acetyl-CLEFLRRFSRVAEETIDPKEY-amide), and rabbit polyclonal affinity–purified anti-lamin1 (acetyl-QSPISLPPLSGSTC-amide) were produced by 21st Century Biochemicals (Marlboro, MA). Other antibodies included anti-Histone H3-pT3 (Abcam), anti-Histone H3-pS10 (Millipore), anti-Histone H3-pS28 (Abcam), anti-phospho-Ser/Thr-Pro MPM-2 (Millipore), anti-Plk1-pT210 (BioLegend), anti-Aurora A (pT288)/Aurora B (pT232)/Aurora C (pT198) (Cell Signaling Technology), anti-MAPK-pTEpY (pERK1/2) (Promega), anti-PSTAIRE (Abcam), anti-eGFP (AMS Biotechnology), anti-Tubulin (Abcam). Secondary antibodies against rabbit, rat and mouse IgG (conjugated Alexa Fluor 488, 568 or HRP) were from Molecular Probes. For immunofluorescence, primary antibodies were used at 1:100 dilution and secondary antibodies at 1:300 dilution. For western blot, primary and secondary antibodies were used at 1:1000 and 1:5000 dilutions, respectively.
Feng H, & Thompson E.M. (2018). Specialization of CDK1 and cyclin B paralog functions in a coenocystic mode of oogenic meiosis. Cell Cycle, 17(12), 1425-1444.
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4

Immunofluorescence Assay for Mitosis Markers

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Cells grown on coverslips were fixed in 4% paraformaldehyde/PBS for 5 min and permeabilized in 0.1% Triton X-100/0.1% Tween-20 for 5 min. Cells were blocked in 3% bovine serum albumin for 1 h before primary antibody incubation for 2 h at room temperature. Antibodies used were anti–Aurora B pT232 (1:2000; Rockland Immunochemicals, Pottstown, PA), anti–histone H3 pS28 (1:500; 10543; Abcam), and anti–histone H3 (1:500; 46765; Abcam).
Mahen R., Koch B., Wachsmuth M., Politi A.Z., Perez-Gonzalez A., Mergenthaler J., Cai Y, & Ellenberg J. (2014). Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells. Molecular Biology of the Cell, 25(22), 3610-3618.
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