A SY3200 sorter (Sony Biotechnology) was used to separate YFP expressing Lpos from non-YFP expressing Lneg cells in both colonic and ileal single cell suspensions. Settings: 70uM nozzle, 50–52 psi, 77.3 kHz, plates charged with 4000 V. Single cells were selected by their forward scatter area and forward scatter height. Lpos cells were selected by their relative fluorescence at 500–550 nm (excited by a 488 nm laser). The cells were gated to exclude doublets and dead cells, and were sorted against YFP vs. PE channels to collect only YFP expressing Lpos populations directly into RNALater (Sigma) at room temperature. A heterogenous non-YFP expressing Lneg population was also collected for each sample. The gating strategy was changed minimally between samples (Fig. 1 ).
Sy3200 sorter
Manufactured by Sony
The SY3200 sorter is a compact and automated laboratory equipment designed for sorting and separating samples. It features a high-speed sorting mechanism and advanced optics for precise sample identification. The core function of the SY3200 sorter is to efficiently sort and separate samples based on user-defined parameters.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sy3200 sorter
1
FACS Isolation of YFP-Expressing Intestinal Cells
2
Thymus Cell Sorting Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
From the thymus, DP (CD4+CD8+), CD4SP (CD4+CD8–), total iNKT (TCRβintCD1d-PBS57-tet+) or iNKT cell developmental subsets (S1, S2, or S3) were sorted using the Sony SY3200 sorter (Sony). Purity for all cells used was >95%.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!