Anti cd4

Manufactured by Novus Biologicals

Sourced in United States

Anti-CD4 is a monoclonal antibody that specifically binds to the CD4 antigen on the surface of T lymphocytes. It is a commonly used tool in immunological research and analysis.

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Lab products found in correlation

Lsm 700 confocal microscope, by Zeiss (2 mentions) Anti myc, by Takara Bio (1 mentions) Anti glyceraldehyde 3 phosphate dehydrogenase gadph, by Cell Signaling Technology (1 mentions) Ripa lysis buffer, by Cell Signaling Technology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Bca protein estimation kit, by Thermo Fisher Scientific (1 mentions) Fluorescein isothiocyanate (fitc), by Santa Cruz Biotechnology (1 mentions) Anti pd l1, by Thermo Fisher Scientific (1 mentions) Anti ctla 4, by Thermo Fisher Scientific (1 mentions) Anti cd8, by Novus Biologicals (1 mentions) Zen 2 blue edition, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Anti rabbit igg peroxidase antibody, by Merck Group (1 mentions) Superfrost plus microscope slide, by Thermo Fisher Scientific (1 mentions) Target retrieval solution, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Rabbit anti-CD4

3 protocols using anti cd4

Plasmid Transfection and Immunoblot Analysis

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Plasmid transfections were performed using Lipofectamine 2000 (Invitrogen) as per the manufacturer's protocol. Relative levels of different proteins were compared by immunoblot analysis. Respective empty vectors were used to normalize transfection. After 48 h of transfection, cells were lysed in RIPA lysis buffer (Cell Signaling Technology), and protein estimation was done using the BCA Protein Estimation Kit (Pierce Biotechnology, Inc.). The primary antibodies used were anti-Myc (Clontech), anti-glyceraldehyde 3-phosphate dehydrogenase (GADPH) (Cell Signaling Technology), and anti-CD4 (Novus Biologicals). The secondary antibodies used were anti-rabbit/mouse-horse radish peroxidase conjugated (Jackson ImmunoResearch). The proteins of interest were detected with EZ western horseradish peroxidase substrate (Biological Industries, Israel). GAPDH was used as a loading control in all cases.

Singh J., Pandey M., Ramachandran V.G, & Banerjea A.C. (2020). Genetic and Functional Characterization of HIV-1 Vpu from HIV-1-Infected North Indian Population. BioResearch Open Access, 9(1), 209-218.

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Immunofluorescence Analysis of Immune Markers in Gastric Cancer Mucosa

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Mucosa from GC patients was fixed in 10% formalin and embedded in paraffin. Paraffin-embedded sections were probed with anti-CD4⁺ (Novus Biologicals, Littleton, CO, USA), anti-CD8⁺ (Novus Biologicals), anti-PD-L1 (Invitrogen, Carlsbad, CA, USA), and anti-CTLA-4 (Invitrogen) primary antibodies at 4°C overnight. They were then stained with secondary antibodies conjugated with fluorescein isothiocyanate (Santa Cruz Biotechnology, Santa Cruz, CA, USA), allophycocyanin (Invitrogen), and phycoerythrin (Southern Biotech, Birmingham, AL, USA) at room temperature for 2 h. Nuclei were stained with 4,’6-diamidino-2-phenylindole (DAPI; Invitrogen). Immunofluorescence images were obtained using an LSM 700 confocal microscope (Zeiss, Oberkochen, Germany) at 200× magnification. Images were analyzed using ZEN 2 (blue edition) (Zeiss).

Lee K.H., Kim S.J., Woo J.S., Lee S.Y., Jhun J., Moon J., Jung Y.J., Cho M.L, & Song K.Y. (2023). Prognostic significances of PD-L1- and CTLA-4-positive T cells and positive correlations of immunosuppressive marker expression between cancer tissue and peripheral blood in patients with gastric cancer. Frontiers in Immunology, 14, 1138743.

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Immunohistochemical Analysis of Tumor-Infiltrating Immune Cells

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SW-1 bearing mice were euthanized 7 days after their tumors had been injected once with the 3 mAb injection, and the tumors were fixed in 10% paraformaldehyde. Four-micron sections of formalin-fixed paraffin-embedded tissue were cut and placed on Superfrost Plus microscope slides (Fisher Scientific, Pittsburgh, PA). The sections were deparaffinized and rehydrated through graded alcohols. Antigen retrieval was carried out with Target Retrieval Solution (DAKO Corporation; Carpinteria, CA) in a microwave for 10 minutes. Endogenous peroxidase activity was blocked with 3% H₂O₂. The slides were washed and incubated with anti-CD4 (Novus biologicals, Littleton, CO), anti-CD8 (Biorbyt, San Francisco, CA) or anti-CD19 (Biorbyt, San Francisco, CA) mAbs overnight at 4°C, after which they were washed and incubated with Anti-Rabbit IgG-Peroxidase antibody (Sigma-Aldrich, St.Louis, MO). Color development was accomplished by incubation in diaminobenzidine (DAKO Corporation; Carpinteria, CA). The slides were counterstained by hematoxylin (DAKO Corporation) and coverslipped with permanent mounting media.

Dai M., Yip Y.Y., Hellstrom I, & Hellstrom K.E. (2014). Curing mice with large tumors by locally delivering combinations of immunomodulatory antibodies. Clinical cancer research : an official journal of the American Association for Cancer Research, 21(5), 1127-1138.

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