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Allplex respiratory panels 1 3

Manufactured by Seegene

The Allplex Respiratory Panels 1–3 are multiplex real-time PCR assays developed by Seegene for the qualitative detection of multiple respiratory pathogens in a single test. The panels can identify various viruses and bacteria associated with respiratory infections.

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2 protocols using allplex respiratory panels 1 3

1

Rapid Viral Detection in Olympic Athletes

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During the Games, an automated POCT, FilmArray Respiratory Panel 2 plus (BioFire; Salt Lake City, UT, USA), was used according to the manufacturer’s instructions. The panel detects the following viruses: respiratory syncytial virus A and B, adenovirus, influenza A and B viruses, rhinovirus or enterovirus (without specifying which), parainfluenza type 1–4 viruses, human coronaviruses 229E, OC43, HKU1, and NL63, and human metapneumovirus. After a processing time of 60 minutes, the test reading was recorded.
Laboratory testing was carried out uniformly in Turku for the samples of the Team members and the subjects of the control group. Two PCR based tests were used: 1) Allplex Respiratory Panels 1–3 (Seegene, Seoul, South Korea) for respiratory syncytial virus A and B, adenovirus, influenza A and B viruses, rhinovirus, enteroviruses, parainfluenza type 1–4 viruses, human coronaviruses 229E, OC43, and NL63, human bocavirus, and human metapneumovirus; 2) in-house triplex RT-PCR assay for respiratory syncytial virus, rhinoviruses, and enteroviruses [10 (link)]. The viral shedding was calculated as reported earlier [11 (link)].
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2

Rapid Diagnostic Tests for HRSV and Influenza

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During the HRSV and influenza epidemics, rapid tests were performed for a fast diagnosis, which were based on immunocromatography (Alere BinaxNOW® Influenza A&B/RSV, Alere, USA), immunofluorescence (Sofia RSV FIA, Quidel, USA) or real-time RT-PCR (GenXpert Flu/RSV XC, Cepheid, USA). Samples received out of HRSV/FLUV epidemics or negative for rapid tests were analysed by immunofluorescence (D3 Ultra 8™ DFA Respiratory Virus, Diagnostic HYBRIDS, USA) or mainly by real-time RT-PCR (Anyplex II RV16, Seegene, Korea, until 2015; Allplex Respiratory Panels 1-3, Seegene, Korea, since 2015). Total nucleic acids were extracted using NucliSense easyMAG (BioMérieux, Marcy l’Etoile) and kept at -80 °C.
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