Rabbit anti h3k27ac

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-H3K27ac is a laboratory reagent used for the detection and quantification of acetylated lysine 27 on histone H3 protein. It is a polyclonal antibody raised in rabbits against a synthetic peptide corresponding to the acetylated lysine 27 region of histone H3.

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H3K27ac (74 citations) Anti-H3K27ac (38 citations)

3 protocols using rabbit anti h3k27ac

Epigenetic Landscape Profiling via CUT&Tag

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The eye and wing discs for the CUT&Tag assay were isolated from w¹¹¹⁸, Dmp18^d1, and H2Av⁸¹⁰ third instar larvae. The CUT&Tag was performed using the Hyperactive In-Situ ChIP Library Prep Kit for Illumina (TD901, Vazyme, Nanjing, China). The antibodies for CUT&Tag assay: rabbit anti-H2Av (Active Motif, 39715), rabbit anti-H3K4me3 (Cell Signaling Technology, 9751), rabbit anti-H3K27ac (Cell Signaling Technology, 8173), and mouse anti-H3K27me3 (Abcam, ab6002). The sequencing was performed using the Illumina Novaseq 6000 platform. The raw NGS data were deposited to the NCBI SRA database under Bioproject ID: PRJNA761186 (the accession numbers are from SRR15734939 to SRR15734950).

Feng Y., Zhang Y., Lin Z., Ye X., Lin X., Lv L., Lin Y., Sun S., Qi Y, & Lin X. (2022). Chromatin remodeler Dmp18 regulates apoptosis by controlling H2Av incorporation in Drosophila imaginal disc development. PLoS Genetics, 18(9), e1010395.

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Macrophage Epigenetic Regulation Study

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BMDMs were treated as indicated. Whole-cell lysates were prepared in RIPA buffer and subjected to western blotting. The antibodies used were rabbit anti-HP1-α, rabbit anti-H3K4me3, rabbit anti-H3K9me3, rabbit anti-H3K27me3, rabbit anti-H3K27Ac, rabbit anti-H, rabbit anti-NF-B, rabbit anti-ERK, rabbit anti-MAPK, rabbit anti-p-SAPK-JNK, rabbit anti-NF-κB, rabbit anti-ERK, rabbit anti-MAPK, rabbit anti-SAPK-JNK, mouse anti-actin, HRP-conjugated donkey anti-rabbit IgG and HRP-conjugated sheep anti-mouse IgG (all purchased from Cell Signaling Technology, Danvers, MA, USA). The signals were detected by chemiluminescence. The protein bands intensities were quantitated using ImageJ Gel Analysis program. The modified proteins were normalized to their loading controls (total forms) and relatively normalized to those of the unstimulated cells.

Boonmee A., Benjaskulluecha S., Kueanjinda P., Wongprom B., Pattarakankul T, & Palaga T. (2021). The chemotherapeutic drug carboplatin affects macrophage responses to LPS and LPS tolerance via epigenetic modifications. Scientific Reports, 11, 21574.

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Western Blotting of Protein Lysates

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Protein lysates were separated on 4–20% SDS/PAGE gels and transferred to PVDF membranes. Western blotting was performed using Fast Western Blot kits (Thermo Scientific Pierce) with the following antibodies: mouse anti‐FLAG M2 antibody (Sigma, F1804), rabbit anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH; Cell Signaling Technology, 2118), rabbit anti‐APEX1 (NOVUS, NB100‐101), mouse anti‐HDAC1 (NOVUS, NB100‐56340SS), rabbit anti‐H3K27ac (Cell Signaling Technology, 9733S), and mouse anti‐vimentin (Abcam, ab8978).

Zhang Q., Chao T., Patil V.S., Qin Y., Tiwari S.K., Chiou J., Dobin A., Tsai C., Li Z., Dang J., Gupta S., Urdahl K., Nizet V., Gingeras T.R., Gaulton K.J, & Rana T.M. (2019). The long noncoding RNA ROCKI regulates inflammatory gene expression. The EMBO Journal, 38(8), e100041.

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